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TEST STATUS: OBSOLETE NOTIFICATION DATE: June 11, 2015 EFFECTIVE DATE: June, 26, 2015 HIV-1 Proviral DNA Qualitative Detection by PCR, Blood Test ID: PHIV EXPLANATION: The manufacturer of this reagent has chosen to discontinue production. Effective June 26, 2015 this test code will become obsolete. RECOMMENDED ALTERNATIVE TEST: HIV-1 RNA Detection and Quantification, Plasma Test ID: HIVDQ USEFUL FOR: x Diagnosis of HIV-1 infection in individuals with acute or early HIV-1 infection. x Diagnosis of HIV-1 infection in infants of <18 months of age born to HIV-1-infected mothers x Quantifying plasma HIV-1 RNA levels (viral load) in HIV-1-infected individuals: -Before initiating anti-HIV-1 drug therapy (baseline viral load) -Who may have developed HIV-1 drug resistance while on anti-HIV-1 therapy -Who may be noncompliant with anti-HIV-1 drug therapy Monitoring HIV-1 disease progression while on or off antiretroviral drug therapy x METHOD: Real-time reverse transcription-polymerase chain reaction (RT-PCR) REFERENCE VALUES: Undetected SPECIMEN REQUIREMENTS: Container/Tube: Lavender top (EDTA) Specimen Volume: 2.0 mL Collection Instructions: Spin down and remove plasma from cells within 6 hours of draw. Minimum Volume: 1.2 mL SPECIMEN STABILITY INFORMATION: Specimen Type EDTA Plasma Temperature Frozen (Preferred) Refrigerated Time 35 days 5 days CAUTIONS: This test is not licensed by the FDA as a screening test for HIV-1 infection in donors of blood, human cells, tissues, or tissue products. CPT CODE: 87536 DAY(S) SET UP: Monday through Saturday ANALYTIC TIME: 1 day QUESTIONS: Contact your Mayo Medical Laboratories’ Regional Manager or Kim J. Baker, MML Laboratory Technologist Resource Coordinator Telephone: 800-533-1710