Download Factors Affecting Enzyme Activity

Factors Affecting
Enzyme Activity
10-1 :
Munich, Anne, Fluke, and Yu
Factors Affecting
Enzyme Activity
• We are doing this experiment in
order to test and find out the factors
that has affects on the enzyme.
• The indicator tells the substance’s
chemical characteristic(pH). For red
cabbage indicator
–Acid : within red range
–Base : blue to green
–Neutral : no change(violet/blue)
Procedure 1
Tube1
(indicator and
citric acid)
Tube3
(indicator and
distilled water)
red
Tube2
(indicator and
sodium
bicarbonate)
greenblue
acid
base
neutral
violet
Why does it matter
to do this part?
• We have already known the chemical
properties of the substrates we add.
Citric acid is acid, Sodium bicarbonate
is basic, and Distilled water is neutral.
• They should change the color
properly related to their properties
• We can predict that the color of
solution will change to blue green
once the urease reacts with urea
Procedure 2
Tube Indicator
Yes
Yes
Yes
1
2
3
Tube
1
2
3
Urea
solution(sub)
Yes
Distilled
water
-
Soya
suspension(enzyme)
Yes
Yes
-
Yes
Yes
Property
Color before
add urease
Enzyme+Subtrate
violet
Substrate
Enzyme
violet
violet
Final color
Green with
slightly blue
violet
Slightly turbid
violet
Tube 3
Tube 1
Tube 2
• Purpose : to see the chemical
reactions between substrate and
enzyme
• We add urease into the tube without
urea because it is the control
variable.
Procedure 3
Tube
Place
Temp.
Final color
Ice bath
0°C Slightly turbid green
1
Room
22°C
Green
2
Water bath 63°C
Dark green
3
Hot plate
84°C
Violet with dregs
4
Boiling water 100°C Violet with dregs
5
Room temp
Ice bath
Water bath
Hot plate
Boiling water
• The temperature affects the
urease enzyme differently.
• We can see from its color and the
protein denature.
• We should use the optimal
temperature (20-25°C).
• The enzyme will work properly, and
provide the good result.
• Avoid the high and low temperature
because the protein will be denature,
or slower the reaction.
• When the enzyme is denatured, the
protein will become solid, form the
dregs, and shrink to the bottom
Conclusion
• Enzyme works differently in each
condition.
• High temperature can cause the
enzyme to denature.
• Low temperature slower the
reaction.
• The enzyme activity will occur when
it has the correct pair of enzyme and
substrate.
• We can see its function by using the
indicator to tell its properties. If it
turns to green or blue, it means the
ammonia is produced.