Download BIOLOGY -ENZYME LAB- (Effect of temperature)

BIOLOGY
-ENZYME LAB(Effect of temperature)
Group Member (10/10)
Sittipun Suwanmanee
Kammanat Lohisathan
Natwadee Pattanaphnitchkul
Sasicha siriwong
Thanaphat Tangroek
Wassawan sangkapong
Aim of the experiment
• To find out the effect of temperature
on enzyme activity.
Background
• In this experiment we used urease
enzyme in soya bean to breakdown
urea into ammonia and carbon
dioxide.
• We can detect the presence of
ammonia by using red cabbage
indicator.
Hypothesis
• We predict that enzyme will denatured in
high temperature and in low temperature
enzyme activity will occur slowly.
Result : Procedure 1
Indicator + water
(neutral)
Indicator + sodium
bicarbonate
(Alkaline)
Indicator + citric acid
(Acid)
purple
Dark blue
Red - pink
Why is it important to have the
three reference test tubes?
• We can indicate whether our results are
acid, alkaline or neutral by compare the
colour to the reference test tubes.
• In this experiment positive result should
turn blue in colour since ammonium
solution is alkaline.
Result : Procedure 2
Indicator + Urea
Solution
purple
Indicator + Urea
Solution + Soya
Suspension (Enzyme)
Dark Blue
Indicator + distilled
water + Soya
Suspension (Enzyme)
Purple
Purposes
• To see whether urease enzyme is working.
Only the test tube that contain both
enzyme and substrate should show
positive result.
Why do we need to add an enzyme into
a test tube without urea even though we
can predict that no enzyme activity will
occur?
Because we need to know that whether the
indicator going to change the colour when
mix with the enzyme. If the solution turn
blue then we will have to modify the
experiment.
Result : Procedure 3
Placement
Temperature( °C )
clarity
colour
Ice bath
0
clear
blue
Beaker on hot
plate at 245 °C
85
opaque
Purple
Beaker on hot
plate at 360 °C
87
opaque
purple
Room
temperature
22
clear
Blue (before Ice
bath)
Boiling water
100
opaque
purple
• Cold temperature slow down the
enzyme activity since the solution
slowly turn blue.
• Test tube in room temperature turn
blue first which mean it is the most
suitable temperature to use urease.
Room
Temp
Ice Bath
• High temperature can cause enzyme
to denature. We can tell since the
solution in high temperature did not
turn blue in color.
• Denatured enzyme caused solution
to turn opaque.
Extra information
• Optimum temperature of urease
activity is around 45 – 60 °C
• We can use this range of temperature
to maximize urease activity.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442171/
CONCLUSION
Conclusion
• Our hypothesis is true since test tubes
that are in high temperature did not
show positive result for enzyme
activity. Test tube in the ice bath
turned blue eventually and the one
that turned blue first is the one in
room temperature beaker.
Conclusion
• Enzymes work best in their optimum
condition (optimum temperature for
this experiment) Too high
temperature will cause them to
denature and low temperature slow
enzyme activity down because
particles move slowly in low
temperature.
Thank You