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Passive Transport Diffusion Osmosis Biology Unit - Regulation Diffusion • Involves the movement of molecules from an area of high concentration to an area of low concentration • Does not require energy (ATP) Examples of Diffusion Diffusion • The movement of a substance down its concentration gradient Equilibrium • even at equilibrium the molecules move at random Equilibrium Osmosis Is all about the movement of WATER molecules Learning Osmosis • Water molecules diffuse from an area of low solute concentration to an area of high solute concentration Learning Osmosis • Water molecules diffuse from an area of high water concentration to an area of low water concentration Isotonic • When the concentration of solutes outside and inside the cell are equal. Hypertonic • When the concentration of solute molecules outside the cell is higher than the concentration in the cytosol • The solution outside the cell is hypertonic to the cytosol Hyper = high Hypertonic Hypotonic • When the concentration of solute molecules outside the cell is lower than the concentration in the cytosol • The solution outside the cell is hypotonic to the cytosol Hypo = low Hypotonic Effect of Solutions on Cells How do cells deal with Osmosis? 1) cells in hypotonic environment unicellular freshwater organisms (paramecium) – water constantly diffuses in – They require low concentration of water in cytosol – need to get rid of excess water How do cells deal with Osmosis? – do this with contractile vacuoles • organelles that remove water • collect water and pump it out of cell How do cells deal with Osmosis? 2) some cells cannot compensate for changes in solute concentration – red blood cells lose their normal shape when placed in an environment that is not isotonic to their own Normal Cells in Isotonic Solution Cells in Hypertonic Solution Cells in Hypotonic Solution Photomicrograph of Photomicrograph of blood cells in an isotonic blood cells solution. in a hypertonic solution; the puckered effect comes from the loss of fluid from inside the cell. Photomicrograph of blood cells in a hypotonic solution; the bloated effect comes from the swelling of the cell. Alright…. Let’s test your new knowledge…. What has happened to this cell? Explain.