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WHERE DOES IT HAPPEN? The stroma, a fluid filled compartment where sugars are made from carbon dioxide, that is located in the choroplast. What Happens? The enzyme rubisco combines carbon dioxide with RuBP, a five-carbon sugar molecule. This process is called CO2 fixation. As this product is an unstable six carbon compound it breaks down to form two molecules of three carbon glycerate 3-phosphate (GP). GP then can be used to form Amino acids and fatty acids. Amino Acids GP Fatty Acids GP is reduced and phosphorylated to triose phosphate. ATP and reduced NADP from the light dependent stage are used in this process. Five out of every six TP’s are recycled back RuBP using ATP. ATP 2GP Reduced NADP ADP+Pi Triose phosphate NADP+ How the products can be used Two TP molecules will form hexose sugars such as glucose, which can be further isomerised into fructose. Sucrose is another product. Glucose + Fructose Sucrose. Polymerised hexose sugars can be form carbohydrates such as cellulose and starch. Glycerol from TP and fatty acids from GP may combine to form lipids.