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DNA Isolation Get Started Read the introduction. (p. 328) What is DNA? Deoxyribonucleic acid (DNA) is a molecule that makes up genes, present in every living organism. Activity 9: DNA Isolation Three Types of Cells Where is DNA in each of these cells? Bacteria Cell Plant Cell Activity 9: DNA Isolation Typical Animal Cell Challenge How could you isolate DNA from an organism’s cell? Activity 9: DNA Isolation Procedure Need a source of DNA Lysis is the process of breaking cells apart. Precipitation occurs when a dissolved substance is made insoluble, usually by adding a chemical. The substance becomes a solid and precipitates out of the solution. Activity 9: DNA Isolation Follow Up Complete Analysis p. 327 (1-2-4-5) Analysis 1: What is the purpose of mashing the sample in the plastic bag? Breaks down the cell & nuclear membranes Activity 9: DNA Isolation Follow Up Analysis 2 Describe the purpose of the lysis solution & the DNA precipitation solution Lysis solution: (salt & detergent)…breaks down the cell & nuclear membrane Precipitaiton solution (alcohol)…caused the DNA to clump together Activity 9: DNA Isolation Follow Up Analysis 4 Compare the properties of DNA isolated from different organisms. What similarities did you observe? Similar in color & consistency Activity 9: DNA Isolation Follow Up Analysis 5 How is the mechanism for isolating DNA from an organism connected to the mechanism for genetically modifying that organism? DNA must first be isolated before it can be removed & placed into another organism Activity 9: DNA Isolation
