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CellPowerTM Online Quotation Form (SC1394) Instructions 1. Please complete and email this form to [email protected]. Our service representative will contact you with the quote. 2. Please complete all fields with asterisk* Customer Information Name*: Phone: Institution*: Shipping address* (needed to determine shipping cost): Email*: Project Objective* Over-expression of Gene of Interest (GOI) Knock-down Expression of GOI Lentivial Inducible TetOn system Lentivial Inducible TetOff system Other Project Information Is this project for grant application purpose? When will the project start*? Immediately Yes No Within one month Within three months Gene Information Gene Name*: DNA sequence: Gene Accession Number*: Has the gene been studied for stable expression previously*? Yes, please provide reference if yes: ___ No Will you be able to provide sequence report*? Yes No Who will provide the gene*? GenScript Client Note: Customers will be additionally charged for DNA synthesis Do you need a tag* (His, Flag, HA, myc, GFP, RFP, etc.)? No, do not include any tags. Yes, please specify the tag you prefer:_____ N-terminal C-terminal Internal Not sure Half a year later Do you want to express multiple genes in one vector 2A self-cleaving peptide internal ribosomal entry site (IRES) elements double promoters No, I don’t need it Which one would you choose*? Stable Pool Single Clone Both Will serial dilution affect cells growth condition*: Yes No Unknown Cell line information Name of host cell line*: Who will provide host cell line*? GenScript Client Culture type of host cell line*: Adherent Suspension Half adherent and half suspension Not sure Please specify the culture condition here:____ Could you please describe what your application is with this cell line? Gene function analysis Assay development Drug screening Bio production Other. Please indicate your specific application and requirements: ____ Comments: How to introduce a foreign gene into the host cells* Lentivirus Plasmid transfection (or electroporation if available) I want GenScript to recommend the method Validation methods you prefer* Q-PCR Flow cytometry: I can provide a specific antibody I can’t provide a specific antibody Western blotting: I can provide a specific antibody I can’t provide a specific antibody Luciferase assay Calcium influx assay or cAMP assay Others, please specify ____ Additional requirements or comments