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Inhalation System for Animal Infection With BSL3 Pathogens Team Members: Stacey Hoebel Maja Middleton Alternative Design: One-Compartment Box Modification of current lab design – Safer and more efficient One large compartment made of plastic able to withstand autoclave – No need for individual compartments for animals – Small enough to fit under fume hood Alternative Design: One-Compartment Box Tube from nebulizer into compartment to administer pathogen Tube from compartment to ethanol trap and HEPA filter Alternative Design: One-Compartment Box Pros Very inexpensive Easy to manufacture Better quality – Plastic compartment – Seal around tubing and lid Cons Too simple Doesn’t address all problems – Contaminates entire body of animal – Inconsistent amount of infection administered to animals Alternative Design: Central Porous Tubing Plastic box with lid – Split into six built-in compartments which hold one animal each Central Porous Tubing – One tube runs from nebulizer then down middle of box, between compartments – Infectious gas expelled out of holes in tubing into individual chambers – Remaining gas led to HEPA filter and ethanol trap Restraint Device – Holds animal in place while being infected Alternative Design: Central Porous Tubing Alternative Design: Central Porous Tubing Pros Individual compartments and restraints keep animals separated, calm Better control of agent Relatively inexpensive Easy to manufacture Cons Not accurate enough – Impossible to regulate how much agent is administered to each animal Final Design: Multi-Chamber w/ Nose Piece Plastic box with lid – Made of polymethyl pentene (autoclavable) – Split into four builtin compartments Moveable back wall to adjust to animals of various sizes – Small enough to fit under fume hood Final Design: Multi-Chamber w/ Nose Piece Split Tubing – Nebulizer leads into one tube – Router splits this tube into four separate tubes – Each tube leads to a different compartment – Another router condenses tubing into one tube leading to ethanol trap and HEPA filter Final Design: Multi-Chamber w/ Nose Piece Neck Seal – “Wall” of stretchy material set 1.5 inches from front of compartment – Small hole in center of material for head and neck – Holds animal in place – Creates seal between head and body Final Design: Multi-Chamber w/ Nose Piece Nose piece – Cone formed at end of tubing by snipping each side and caulking between cut – Does not need to be directly attached to head – Directs airflow to nose and mouth Final Design: Multi-Chamber w/ Nose Piece Pros Separate tube for each chamber – Consistent, efficient administration of pathogen Minimization of body contamination – Nose piece directs airflow – Seal between head and body Able to sanitize entire system Many safeguards to prevent leakage of agent Final Design: Multi-Chamber w/ Nose Piece Future Work Consult client about possibly anesthetizing animals – Complicated to monitor sedation levels – Could lose some animal subjects Organization system for all the tubing Appropriate materials (able to sterilize, non-porous) Questions?