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Pharmacologyonline
Volume 3, September 2009, Pages 1062-1069
Study the cytotoxic and pro-apoptotic activity of Artemisia
annua extracts
Emami, S.A.a, Rabe, S.Z.T.b, Ahi, A.a, Mahmoudi, M.b , Tabasi, N.b
a Department of Pharmacogneosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
b Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad,
Iran
View references (18)
Abstract
Growth inhibitory activity of Artemisia species on gastric cancer cells have been reported. This study focused on
growth inhibitory activity of different extracts from Artemisia annua on gastric cancer cells. Methanol, ethylacetate,
dichloromethane and hexan extracts were prepared from aerial parts of Artemisia annua by step to step procedure.
Cultivated gastric cancer cell line (AGS) and normal fibroblast cell line (L929) were incubated with different
concentrations of extracts for 24 hours and cell growth inhibition was determined using MTT assay. Induction of
apoptotic and/or necrotic death in AGS cells was evaluated using Annexin V and propidium iodine staining method.
Cytotoxic activity of extracts was shown as growth inhibition percent of treated cells compared to untreated cells.
Prepared extracts from Artemisia annua showed significant inhibitory effect on AGS cancer cells mainly by induction
of apoptosis. They showed less inhibitory effect on L929 normal cells. Methanol extract showed the highest growth
inhibitory effect (IC 50: 500 μg/ml) on AGS cells and it induced apoptosis more than others. So, evaluation of
Artemisia annua in prevention or treatment of gastric cancer is recommended. Also, isolation of effective compound/s
from this extracts and determination of their mechanisms of actions is suggested.
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Author keywords
Artemisia annua; Cell death pattern; Fibroblast cell line; Gastric cancer cell line; Growth inhibitory activity
Indexed Keywords
EMTREE drug terms: acetic acid ethyl ester; antineoplastic agent; Artemisia annua extract; dichloromethane;
lipocortin 5; methanol; nitroblue tetrazolium
EMTREE medical terms: animal cell; antineoplastic activity; Artemisia annua; article; cancer cell culture; cancer
inhibition; cell death; comparative study; controlled study; cytotoxicity; drug activity; drug effect; drug inhibition; drug
mechanism; fibroblast culture; human; human cell; incubation time; nonhuman; pro apoptotic activity; staining;
stomach cancer
Chemicals and CAS Registry Numbers: acetic acid ethyl ester, 141-78-6; dichloromethane, 75-09-2; lipocortin 5,
111237-10-6; methanol, 67-56-1; nitroblue tetrazolium, 298-83-9
ISSN: 18278620Source Type: Journal Original language: English
Document Type: Article