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25th Croatian meeting of chemists and chemical engineers, Poreč, 2017. A Fluorescent Probe for Specific Detection of Hydrogen Sulfide Based on a Familiar ESIPT Fluorophore bearing AIE Characteristics Liyan Chen,1 Di Wu, 1* Woolin Lee1 and Juyoung Yoon1* 1 Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Korea E-mail:[email protected] Hydrogen sulfide (H2S) which has been known as a rotten eggs smell gas has been identified as the third gasotransmitter following nitric oxide (NO) and carbon monoxide (CO). In quite a long time, hydrogen sulfide was considered to be an absolute toxic gas and environmental pollutant. However, this view was modified as H2S was first observed to present as a neuromodulator in the brain in 1996. As a matter of fact, hydrogen sulfide is also the smallest member among reactive sulfur species (RSS), making major contributions to the function of mammalian tissues. For example, the imbalance of hydrogen sulfide in living system may be harmful to peripheral and central nervous systems, leading to Down’s syndrome, Huntington’s, Parkinson’s, and Alzheimer’s diseases. Accompanied by the critical biological function of hydrogen sulfide, the chemical detection method of hydrogen sulfide in vivo has attracted considerable attention. Consequently, sustained research efforts have been devoted to the development of efficient strategies for detection of hydrogen sulfide. In contrast to the electro-chemical methods, gas chromatography, the fluorescent probes based on small molecules have been more attractive owing to its simplicity, high sensitivity, noninvasiveness, and real-time detection in living systems. Recently, a large number of fluorescent probes for H2S have been developed. However, most of these probes are based on fluorophores which may suffer from small Stokes shift and/or undergo aggregation caused quenching (ACQ) in aqueous or physiological media. Hydrogen sulfide (H2S) is a signaling gasotransmitter which fulfills various roles in modulating the functions of different systems. In this study, we developed a "turn-on" fluorescent probe for H2S based on a familiar ESIPT fluorophore bearing AIE characteristic. The probe exhibited 80-fold fluorescent enhancement upon addition of H2S with large Stokes shift. Furthermore, the probe has been successfully applied to detecting H2S in HeLa cells. Figure 1: Probe for Detection of H2S Based on ESIPT Fluorophore bearing AIE Characteristics References [1] Y. L. Pak, J. Li, K. C. Ko, G. Kim, J. Y. Lee and J. Yoon, Anal. Chem., 88(2016), 5476-5481. [2] D. Wu, L. Chen, N. Kwon and J. Yoon, Chem, 1 (2016), 674-697.