Download 281008164230Hyaluronic Acid Conjugated Chitosan Gold Nano

Hyaluronic Acid Conjugated Chitosan Gold Nano Particles For
Targeted Drug Delivery.
Radhakumary, C, Prabha D Nair.
Laboratory for Polymer Analysis, BioMedical Technology Wing, Sree Chitra
Tirunal Institute for Medical Science and Technology, Thiruvananthapuram695012, India.
Objective:
Hyaluronic acid conjugated chitosan(CH-HA) encapsulated gold nano particles
have been prepared and characterized as targeted drug delivery system.
Background:
A long standing problem in cancer chemotherapy is the lack of tumor specific
treatments [1]. Tumor cells over express many tumor–specific receptors, which
can be used as targets to deliver cytotoxic agents into tumors [2]. Hyaluronic acid
is one of such receptors which can be applied as tumor–specific moiety to
construct ‘guided molecular missiles’.
Results:
In the present study gold nano particles were prepared using different
concentrations of chitosan. Chitosan encapsulated gold nano particles were
conjugated with hyaluronic acid using water soluble carbodiimide. The UV-Vis
absorbance spectra and infra red spectra were recorded to confirm the formation
of the nano particles. The morphology of the nano particles were observed using
transmission electron microscope at 50000 X. The particle size and zeta potential
of the nano particles were also determined. The chitosan encapsulated gold
nano particles were nearly 22 nm in size and the size increases considerably
when hyaluronic acid is conjugated onto chitosan. It is expected that the drug
loaded biofunctionalised chitosan encapsulated gold nano particles will enhance
the cellular apoptosis of tumor cells.
Conclusion:
Use of chitosan serves dual purpose by acting as a reducing agent in the
synthesis of gold nano particles and also provides as a biocompatible and
biodegradable polymer which promotes the uptake of drugs. The CH-HA
nanoparticles provide bright prospects for the development of highly efficient
matrices for targeted drug delivery for cancer therapy, growth factor delivery for
tissue engineering and so forth.
References:
1. I. Ojima, Accounts of chemical research, 41, 1,108-119, January 2008.
2. S. Jaracz, J. Chen, L.V. Kuznetsova, I. Ojima, Bioorg.Med.Chem, 13,
5043-5054, 2005.