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Animal Growth and Development What is the Different between Growth and Development?? Animal Development • Introduction to animal development Development requires both cell growth and cell differentiation (different cells express different genes) How are new organisms produced? Fertilization • In mammals, sperm travel from the vagina to the fallopian tube • Fertilization takes place in the fallopian tube to form Zygote Once fertilization ends… What’s next? How many step happen in animal growth and development? TWO PHASES 1.Embryonic Phase 2.Pasca Embryonic Phase Egg and Zygote Polarity • Most animals have eggs and zygote with polarity: Animal pole: low concentration of yolk. Vegetal pole: yolk concentrated here 1. EMBRYONIC PHASE After fertilization…there are 3 major steps of embryonic phase: • • • • Morulation Blastulation Gastrulation Differentiation/ Organogenesis Animal Embryonic Development • Stages of development 1. Early Cleavage (Morulation) • Cleavage begins within an hour of fertilization; series of mitotic divisions • Zygote divides into 2, 4, 8, … smaller and smaller blastomeres without increasing size of embryo • Cleavage involves about 12 divisions resulting in solid ball of blastomeres How are new organisms produced? In Amphibians Completed in 24 hrs. 1 and 2 plane is meriodional; 3 equatorial 16-64 cell stage – morula 128 – 15,000 cell stage – blastula st nd rd is 2. Cleavage - blastulation • rapid cell divisions • little cell growth • packaging of cytoplasmic heterogeneity • final product is a hollow ball of cells = blastula cells = blastomeres hollow cavity = blastocoel Cont… • yolky eggs alter pattern of divisions animal hemisphere divides normally vegetal (yolky) hemisphere • divides less often • produces larger cells • amount of yolk affects cleavage pattern 3. Gastrulation • Gastrulation - sorts • all the cells into distinct cell layers (ectoderm, endoderm, and mesoderm) Blastula (hollow ball of cells) transformed into the Gastrula (three layered stage) Gastrulation • 1. Gastrulation begins- Blastopore formed Blastopore - midway opening on one side of the blastula -Site of cell migration from the surface into the interior (Invagination) - Future site of anus • Gastrulation 2. Cell migrating to form layers Archenteron – primitive gut formed (endoderm) future mouth • • Gastrulation 3.Gastrulation complete Gastrula formed Endoderm and archenteron replace the blastocoel Mesoderm - forms a layer between the ectoderm and endoderm Ectoderm- forms the outer layer. Yolk plug- (endoderm) marks the site of the blastopore and of the future anus • • • • Gastrulation in a sea urchin embryo Key Future ectoderm Future mesoderm Future endoderm Animal pole Blastocoel 1 The blastula consists of a single layer of ciliated cells surrounding the blastocoel. Gastrulation begins with the migration of mesenchyme cells from the vegetal pole into the blastocoel. Mesenchyme cells Vegetal plate Vegetal pole Blastocoel 2 The vegetal plate invaginates (buckles inward). Mesenchyme cells migrate throughout the blastocoel. Filopodia pulling archenteron tip 3 Endoderm cells form the archenteron (future digestive tube). New mesenchyme cells at the tip of the tube begin to send out thin extensions (filopodia) toward the ectoderm cells of the blastocoel wall Archenteron Blastopore Mesenchyme cells Blastocoel 50 µm Archenteron Ectoderm Mesenchyme: (mesoderm forms future skeleton) Mouth Blastopore Digestive tube (endoderm) Anus (from blastopore) 4 Contraction of these filopodia then drags the archenteron across the blastocoel. 5 Fusion of the archenteron with the blastocoel wall completes formation of the digestive tube with a mouth and an anus. The gastrula has three germ layers and is covered with cilia, which function in swimming and feeding. WATCH THIS VIDEO …. Differentiation/Organogenesis •Changing process of structure and function become specific tissue. Formation of organ systems from germ layers • Ectoderm •Makes up the outer covering of the gastrula Becomes: Nervous system Epidermis • Mesoderm •Becomes: Muscles Heart Kidney Dermis Circulatory, urinary, reproductive system Endoderm •Becomes: Digestive system Respiratory System Liver Pancreas Pasca Embryonic Phase Maturity and Completing become more perfect organism. There are two divisions Regeneration Metamorphosis 1. 2. 1. Regeneration • Repairing damage/lost organ. Eg. High Animal Asexual Reproduction. Eg. Planaria • 2. METAMORPHOSIS 1. Insect a. Ametabole NO metamorphosis - No Larva Stage - Increasing body size. Eg. Book Lice - b. Hemimetabola • Unperfect Metamorphosis. Stage: Egg Larvae semi-imago imago/adult. Eg. Grasshopper Cockroach c. Holometabola • Perfect Metamorphose Stage: Egg Larvae (Instar) Pupae Imago/Adult. Eg. Butterfly 2. Amphibi • Premetamoprhosis Fertelized egg Kecebong Prometamorphosis Hind Limb appear and grow slowly. Climax Metamorposis Front Limb appear and tail be lost • • Test yourself...