Download COURSE DESCRIPTION

Biotechnology (is a base
for bio industry)
The utilization of living organisms
(micro organisms, plant cells, etc) or
part of living organisms to produce
products
Moleculer Biology
Microbiology
Cell Biology
Moleculer genetics
Biochemistry
Bioprocess Engineering
Biotechnology
Chemical Industry
Health diagnose
Industrial Fermentation/ Bioindustry
Pharmaceutical Industry
Environmental and Energy
Industri Pangan dan Pakan
Examples of Bio industry Products
Cheese, yoghurt, pickles, sauerkraut,
soya sauce, alcoholic beverage (beer,
wine, sake, etc)
Colouring Material (“angkak”)
Biosynthetic vanillin
Thickener (xanthan gum, microbial
alginat, etc)
Single Cell Protein
Probiotic
Organic acids (acetic acid, lactic acid, etc)
Solvents (ethanol, acetone dll)
Amino acids, enzymes, biosurfactant, etc
Flavor Enhancer (MSG, Ribotide)
Arachidonic oil
Transgenic plants (GMO plants)
Antibiotic, Insulin, Interferon,
Steroid
Vitamin (B2, B12, C dll)
Vaccine (e.g Hepatitis B,
influenza, Polio, Meningitis etc)
Food Supplement (Spirulina,
Chlorella)
Indigenous technology :
tempeh, tape, tauco,
Biotechnology development before
Louis Pasteur
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Alcoholic beverages  Beer , Wine, Khamr,
Sake, Tuak, etc (Natural fermentation on sugar
based substrates)
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Fermented Foods (Cheese, yoghurt, tape,
tempeh, petis, terasi, tauco, etc)  milk
derivatives products and solid substrates
technology
Biotechnology Development
Louis Pasteur Era
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Alcohol and its derivatives (Ethanol,
Butanol, aceton, glycerol)
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Organic acids ( Citric acid, acetic acid, etc)
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Waste treatment by aerobic process
Biotechnology Development
Antibiotics Era
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Antibiotics (Penicillin, tetracycline,
streptomycin, etc)  encouraged by world
war
Vaccine (meningitis  firstly isolated from
naso pharynx of an army that was infected
by bacteria)
Biotechnology Development
After Antibiotics
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Amino acid (Glutamic acid, lysine,
aspartame)
Single cell protein
Enzyme (amylase, glucose isomerase,
glucose dehydrogenase, etc)
Immobilized cells and enzymes
Waste water treatment by anaerobic
(Biogas)
Polysaccharide (Xanthan, Pullulan, etc)
Bacterial bio insecticides
Biotechnology Development
Modern Biotechnology
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1973, 1st time gen cloning
1974, expression of gen cloning in other
organisms
1975, bioinsecticides from Bt israelensis  to
eradicate mosquito larvae (Aedes aegepty, Culex,
Anopheles, etc)
Genetic engineering
Level of Biotechnology Application
Level
High
Medium
Low
Input
Output
High investment,
High value
sophisticated equipment
products for health
and process , skilled
and food
operator
Medium investment, not
Food, feed,
too sophisticated
fertilizers and bio
equipment and process
insecticides
Low investment, simple
Low value
process and equipment,
products
unskilled operator
Example of Agricultural
biotechnology
Transgenic – gen from a certain species
which inserted to other species
Example – Gen Cry (Gen coding for protein
crystal which is insecticidal) is inserted
into a certain plant so that the plant
cannot be attacked by agricultural pests
Maize Transgenic  GMO on maize plant by gen
cry from Bacillus thuringiensis
GMO - Genetically modified organisms
Cotton
Normal
Transgenic
 Bt toxin inside can eradicate
corn borer
 The corn plant has capability to
produce Bt toxin due to the inserted
cry gen from Bacillus thrungiensis
Reason of using biotechnology
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Responding the need of human for
foods and feeds and others in a
rapid time and mass production
Environmental friendly
Vanilla planifolia
Vanilla bean  obtained after
years
Bacterial
Enzyme from
Streptomyces
Bioconversion of
ferulic acid
Vanillin  obtained after
several days fermentation
Other Example
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Insulin hormon usually
produces by extraction of it
from pig pancreas
Now  rapid mass
production
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Insulin hormon produced by
recombinant E. coli (GMO)
with the gene from pig
pancreas
Slant Agar
Liquid Media
Inoculum/starter
Seed
Culture
(dormant)
Reactivation
Propagation
Fermentation /Production
Inoculum Development
Fermentor
Downstream
Processing/Recovery
Bio industry
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Cultivation aspects
Scaling Up
Techno economy
Course description of subject “Bio industrial Technology”
COURSE
DESCRIPTION
TIN 330
Bioindustrial Technology
3(2-3)
Course Description
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Knowledge on cultivation process design
covering environmental factors which affect
microbial growth, enzymatic processes ,
bioreactors and instrument, cultivation process,
downstream process and techno-economy
bioprocess aspects.
Holistic understanding on bioindustrial
technology and how to develop bioindustrial
products
Textbooks
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Aiba,S., A.E humprey, dan N.F. Milis.1973. Biochemical Engineering.,
Second Edition. Academic Press, New York
Belter, P.A.,E.L. Cutsler dan H.Wei-Shou.1988. bioseparations :
Downstream Processing for Biotechnology. A Wiley-interscience Publ., New
York
crueger, W and A. Crueger.1984. Biotechnology : A Textbook of Industrial
Microbiology. Science tech., Inc., Madison
Rehm, H.Z and G. Reed.1985. Biotechnology Vol 9.1995. Biotechnology :
Enzyme, Biomass, Food and Feed, VCH Verlagsgeselshaft mbH,Weinheim
Scragg, A.H.1991.Bioreactors in Biotechnology : A Practical Approach>Ellis
Horword, New york
Stanbury, P.F and A. Whitaker.1989.Principles of Fermentation technology.
Pergamon Press.Oxford
Wang, D.I., C.L.Cooney, A.L.Demain,P.Dunnil,A.E.humprey and
M.D.Lilly.1979.Fermentation and Enzyme Technology.John Willey and
Sons, new york
Textbooks
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Prave,P.,U.Faust,W.Sittg and D.A.Sukatsch.1987.Fundamentals of
Biotechnology.VCH Verlagsgeselshaft mbH,Weinheim
Bailley,J.E and D.F.Ollis.1986. Biochemical Engineering
Fundamentals.Second Edition.McGraw-Hill Co., New York
Chaplin,M.F and C.Bucke.1990. Enzyme Technology>Cambridge
university Press, Cambride
Demain,A.L. and N.A. Solomon.1986.Manual of Industrial Microbiology
and Biotechnology. American Societyfor Microbiology.Washington
Margaritis,A. and J.B. Wallace.Novel Bioreactor System and their
Application.J.of Biotechnology.May,1984
Pirt,S.J.1985.Principles of Microbe and Cell Cultivation.blackwell Sci.,
Oxford
Rehm,H.Z and G.Reed.1985.Biotechnology vol 2 : Fundamentals of
Biochemical Engineering. VCH Verlagsgeselshaft mbH,Weinheim
Other references
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L.Hartoto and I.Sailah.1992. Sistem
Bioreaktor.Pusat Antar Universitas
Bioteknologi.IPB, Bogor
Judoamidjojo,R.M.,E.G.Sa’id dan
L.Hartoto.1989.Biokonversi. Pusat Antar
Universitas Bioteknologi,IPB
Mangunwidjaja,D. and A.Suryani.1994.
Teknologi Bioproses. Penebar Swadaya,
Jakarta
Course Outcomes
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Understand scope range and how important to study bioindustrial
technology
Understand cultivation technique and downstream application on
bioindustry
Understand type of bioreactor and its application to many production
process
Understand free enzyme application and immobile enzyme to many
production process
Understand techno-economy aspects in bioindustry
Understand microbes application for many kind of products produced
with optimal cultivation condition, bioreactor uses and efficient
downstream process
Capable to collaborate in a team work
Mastery skill to work in bioindustry laboratory and capable to conduct
microbial cultivation to produce several products
Understand technique for scaling up of bioindustrial technology
Prerequisites by Topic
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Fundamentals of microbial technology
Bioprocess Laboratory
Lecturer Team
1.
2.
3.
4.
5.
MRH (Mulyorini Rahayuningsih) – coordinator
IKS (Khaswar Syamsu)
LBH (Liesbetini Hartoto)
PUR (Purwoko) – laboratory practices
coordinator
Rini Purnawati and Egnawati Sari – laboratory
practices
Major Topics Covered in the Course
Week
Major Topics
Lecturer
1
Introduction (Biotechnology as a base for bioindustry)
MRH
2
Cultivation techniques and downstream processing
IKS
3
Type of bioreactors and example application to many processes
IKS
4
Free and immobile enzyme application to many production processes
LBH
5
Techno economy aspects in bioindustry
LBH
6
Scaling Up in Bioindustrial Technology
IKS
7
Single Cell Protein Technology and its Prospect to be developed in Indonesia
IKS
8-9
Mid Test
10
Bioethanol Production Technology and its Prospect to be developed in Indonesia
LBH
11
Microbial Enzyme Production technology and its Prospect to be developed in Indonesia
LBH
12
Organic acids production technology and its Prospect to be developed in Indonesia
LBH
13
Biopolymer (gum xanthan, pullulan and dextran) production technology and its
to be developed in Indonesia
MRH
14
Antibiotics production technology and its Prospect to be developed in Indonesia
MRH
15
Bioinsecticide production technology and its Prospect to be developed in Indonesia
MRH
16
Arachidonic acid Oil Technology and its Prospect to be developed in Indonesia
MRH
Prospect
Laboratory practices
Week
Topics
Lecturer
2
GLP in Microbiology Lab
PUR
3
Kinetics of Enzymatic Processes
PUR
4-5
Production of citric acid : submerged cultivation and solid
substrates cultivation
MRH
6-7
Production of bioetanol : Immobilized cells and free cells
PUR
10-11
Bioinsecticides production semi solid and submerged cultivation
MRH
12
Scale up technique
Rini P
13-16
Small Project : Development a prospective of ibioindustrial
products :
- Preparation the proposal
- Implementation of Proposal in Lab Work
- Presentation
Supervisor :
PUR, MRH,LBH,
IKS
Assessment
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Mid exam (UTS ) – 30 %
Final exam (UAS) – 30 %
Laboratory Practices (performance and report) –
25 %
Small Project – 15 %