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The Nitrogen Cycle The information and the flow charts below gives some of the interrelationships in the nitrogen cycle. All life requires nitrogen-compounds, e.g., proteins and nucleic acids. Air, which is 79% nitrogen gas (N2), is the major reservoir of nitrogen. But most organisms cannot use nitrogen in this form. Plants must secure their nitrogen in "fixed" form, i.e., incorporated in compounds such as: o nitrate ions (NO3-) o ammonia (NH3) o urea (NH2)2CO Animals secure their nitrogen (and all other) compounds from plants (or animals that have fed on plants). Four processes participate in the cycling of nitrogen through the biosphere: nitrogen fixation decay nitrification denitrification Microorganisms play major roles in all four of these. Reference http://helios.bto.ed.ac.uk/bto/microbes/nitrogen.htm 1 Use the flow chart of the nitrogen cycle (and/or any other suitable diagram) to rearrange these statements into a correct sequence. It might be a good idea to write out the stages (using the numbers) in the form of a cycle. 1 Some nitrogen is fixed by lightning The enormous energy of lightning breaks nitrogen molecules and enables their atoms to combine with oxygen in the air forming nitrogen oxides. These dissolve in rain, forming nitrates that are carried to the earth. 2 Biological nitrogen fixation requires a complex set of enzymes and a huge expenditure of ATP. Although the first stable product of the process is ammonia, this is quickly incorporated into protein and other organic nitrogen compounds. 3 Decomposers break down the molecules in excretions and dead organisms into ammonia. 4 Some bacteria oxidise ammonia (NH3) to nitrites (NO2-). 5 Denitrification reduces nitrates to nitrogen gas, thus replenishing the atmosphere. Denitrifying bacteria live deep in soil and in aquatic sediments where conditions are anaerobic. They use nitrates as an alternative to oxygen for the final electron acceptor in their respiration. 6 Animals eat plant protein and digest it into amino acids 7 Animals convert ammonia into urea 8 Bacteria break down animal and plant protein into amino acids 9 Nitrogen fixers convert atmospheric nitrogen into ammonia 10 Some bacteria oxidise nitrites to nitrates (NO3-). 11 Amino acids are absorbed into the blood of animals 12 Amino acids are made into protein in animal cells 13 Plants convert nitrate ions into ammonium ions and use them to make amino acids 14 Amino acids are made into protein in plant cells 15 Urea is filtered from the blood in the kidney 16 Bacteria deaminate amino acids to make ammonia 17 Animals deaminate amino acids to make ammonia 18 Urea passes out of animal bodies in urine 19 Bacteria break down urea to ammonia 20 Urea travels through the blood from the liver to the kidneys 21 Under great pressure, at a temperature of 600°C, and with the use of a catalyst, atmospheric nitrogen and hydrogen (usually derived from natural gas or petroleum) can be combined to form ammonia (NH3). Ammonia can be used directly as fertilizer, but most of it is further processed to urea and ammonium nitrate (NH4NO3). 2 Try to make links between the topics in the cycle and the rest of the your AS specification. Can you build up a mind map to help link things together to see the ‘global view’? Follow up questions. 1 Identify which statements apply to the following bacteria: Rhizobium Nitrosomonas Nitrobacter 2 What are the problems that are created by the use of nitrogen-based fertilisers? 3 Where are you in the nitrogen cycle? 4. What happens in the following processes: nitrification nitrogen fixation denitrification decomposition? 3