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FLORIDA STATE COLLEGE AT JACKSONVILLE COLLEGE CREDIT COURSE OUTLINE COURSE NUMBER: MLT 2191 COURSE TITLE: Histotechniques I PREREQUISITE(S): None COREQUISITE(S): MLT 2191L CREDIT HOURS: 4 CONTACT HOURS/WEEK: 4 CONTACT HOUR BREAKDOWN: Lecture/Discussion: 4 Laboratory: Other ____________: FACULTY WORKLOAD POINTS: 4 STANDARDIZED CLASS SIZE ALLOCATION: 20 CATALOG COURSE DESCRIPTION: The course will introduce students to the fundamental principles of histologic technology. Included in this course are the laboratory aspects of specimen preparation, fixation, sectioning and routine staining. Laboratory safety, quality assurance, and general health career concepts are covered. This course may be offered by way of Internet. SUGGESTED TEXT(S): Carson, F.L., & Hladik, C. (2009). Histotechnology, A SelfInstructional Text. Chicago, Illinois: American Society for Clinical Pathology: Latest Edition Brown, R. W. (2009). Histologic Preparations: Common Problems and Their Solutions. Northfield, Illinois: College of American Pathologists (CAP): Latest Edition IMPLEMENTATION DATE: Fall Term, 1990 (911) REVIEW OR MODIFICATION DATE: Fall Term, 1997 (981) Fall Term, 2002 (20031) Fall Term 2015 (20161) – Proposal 2015-03 Fall Term, 2015 (20161) – Outline Review 14-15 1 COURSE TOPICS I. Introduction CONTACT HOURS __PER TOPIC__ 10 A. Components of Health Care Delivery System B. Credentialing Process C. Communication Skills D. Professional Conduct E. Computer Applications II. Laboratory Safety A. B. C. D. Chemical Safety Biohazard Safety Emergency Procedures Physical Safety III. Blood Borne Diseases, Including HIV/AIDS A. B. C. D. E. F. 4 Epidemiology Transmission Prevention and Risk Reduction Behavior Changes Policy Development Legal and Ethical Issues IV. Histological Specimens A. B. C. D. 3 4 Sources and Types Procurement of Specimens Kinds of Preparations Labeling/Tracking Requirements V. Solution Chemistry 4 A. Histological Solutions B. Terminology C. Preparation Principles VI. Tissue Fixation 6 A. Fixing Fluids B. Methods and Appropriate Selection C. Washing, Dehydrating and Clearing Methods 2 COURSE TOPICS (Continued) VII. Embedding Methods CONTACT HOURS __PER TOPIC__ 2 A. Embedding Media B. Types of Embedding C. Embedding Procedure VIII. Sectioning 8 A. Equipment 1. Technics 2. Microtome Operation B. Types of Sectioning 1. Frozen 2. Paraffin 3. Nitrocellulose IX. Mounting and Covering 1 A. Mounting Techniques for Section Types B. Covering Techniques for Section Types X. Staining A. B. C. D. 8 Types Preparation - Routine General Procedure Routine Stain Preparation XI. Introduction to Histochemistry 4 A. Inorganic Constituents of Tissue B. Organic Constituents of Tissue 1. Polysaccharides 2. Proteins and Amino Acids 3. Lipids 4. Enzymes XII. Quality Assurance in Histology Laboratory 6 A. Purpose B. Components C. Design 3 PROGRAM TITLE: Histologic Technology COURSE TITLE: Histotechniques I CIP NUMBER: 1351100800 Program Frameworks can be found at the following website: http://www.fldoe.org/workforce/dwdframe/ 4 Florida State College at Jacksonville Course Learning Outcomes and Assessment SECTION 1 Semester Credit Hours (Credit): Contact Hours (Workforce): Course Prefix and Number: MLT 2191 Course Title: Histotechniques I 4 SECTION 2a (To be completed for General Education courses only.) TYPE OF COURSE (Place an “X” in the box next to those that are applicable.) General Education Core (If selected, core discipline area will be identified in Section 4.) General Education (If selected, you must also complete Section 4, Section 5, and Section 8) SECTION 2b TYPE OF COURSE (Place an “X” in the box next to those that are applicable.) A.A. Elective X A.S. Required Course A.S. Professional Elective A.A.S. Required Course A.A.S. Professional Elective Technical Certificate PSAV/Clock Hour/Workforce Development Education Apprenticeship Upper Division/Bachelors Other: SECTION 3 INTELLECTUAL COMPETENCIES (Place an “X” in the box next to those that are applicable.) X Reading X Speaking X Critical Analysis X Qualitative Skills X Writing X Listening X Information Literacy X Ethical Judgement X Scientific Method of Inquiry Working Collaboratively SECTION 4 (To be completed for General Education courses only.) GENERAL EDUCATION DISCIPLINE AREA (Place an “X” in the box next to those that are applicable.) Communications Humanities Mathematics Social and Behavioral Sciences Natural Sciences SECTION 5 (To be completed for General Education courses only.) GENERAL EDUCATION LEARNING OUTCOME AREA (Place an “X” in the box next to those that are applicable.) Communication Critical Thinking Information Literacy Scientific and Quantitative Reasoning Global Sociocultural Responsibility SECTION 6 LEARNING OUTCOMES Demonstrate ability to apply mathematical formulas to problems involving the preparation of histology reagents. Apply knowledge of tissues (gross & microscopic features) to problems in special fixation, processing, and embedding techniques. Compare and contrast routine microtomy to frozen sectioning, to include purpose, type of specimen, handling considerations, biohazards, and staining. TYPE OF OUTCOME (General Education, Course or Program) METHOD OF ASSESSMENT Program Online quizzes, Midterm exam, & final exam. Program Discussion forum, online quizzes, Midterm exam, & final exam. Program Online quizzes, Midterm exam, & final exam. 5 SECTION 6 (Continued) LEARNING OUTCOMES Discuss a routine histology laboratory’s repertoire of special stains, with respect to purpose of the stain and principle of staining reaction. Apply basic concepts of immunology to the use of immunohistochemical reactions. TYPE OF OUTCOME (General Education, Course or Program) METHOD OF ASSESSMENT Program Online quizzes, Midterm exam, & final exam. Program Online quizzes, Midterm exam, & final exam. SECTION 7 Faculty name(s): Jerry Santiago Date: 1-26-2015 CS20150615 6