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FLORIDA STATE COLLEGE AT JACKSONVILLE
COLLEGE CREDIT COURSE OUTLINE
COURSE NUMBER:
MLT 2191
COURSE TITLE:
Histotechniques I
PREREQUISITE(S):
None
COREQUISITE(S):
MLT 2191L
CREDIT HOURS:
4
CONTACT HOURS/WEEK:
4
CONTACT HOUR BREAKDOWN:
Lecture/Discussion:
4
Laboratory:
Other ____________:
FACULTY WORKLOAD POINTS:
4
STANDARDIZED CLASS SIZE
ALLOCATION:
20
CATALOG COURSE DESCRIPTION:
The course will introduce students to the fundamental principles of histologic technology. Included in this
course are the laboratory aspects of specimen preparation, fixation, sectioning and routine staining.
Laboratory safety, quality assurance, and general health career concepts are covered. This course may be
offered by way of Internet.
SUGGESTED TEXT(S):
Carson, F.L., & Hladik, C. (2009). Histotechnology, A SelfInstructional Text. Chicago, Illinois: American Society for
Clinical Pathology: Latest Edition
Brown, R. W. (2009). Histologic Preparations: Common
Problems and Their Solutions. Northfield, Illinois: College
of American Pathologists (CAP): Latest Edition
IMPLEMENTATION DATE:
Fall Term, 1990 (911)
REVIEW OR MODIFICATION DATE:
Fall Term, 1997 (981)
Fall Term, 2002 (20031)
Fall Term 2015 (20161) – Proposal 2015-03
Fall Term, 2015 (20161) – Outline Review 14-15
1
COURSE TOPICS
I. Introduction
CONTACT HOURS
__PER TOPIC__
10
A. Components of Health Care
Delivery System
B. Credentialing Process
C. Communication Skills
D. Professional Conduct
E. Computer Applications
II. Laboratory Safety
A.
B.
C.
D.
Chemical Safety
Biohazard Safety
Emergency Procedures
Physical Safety
III. Blood Borne Diseases, Including HIV/AIDS
A.
B.
C.
D.
E.
F.
4
Epidemiology
Transmission
Prevention and Risk Reduction
Behavior Changes
Policy Development
Legal and Ethical Issues
IV. Histological Specimens
A.
B.
C.
D.
3
4
Sources and Types
Procurement of Specimens
Kinds of Preparations
Labeling/Tracking Requirements
V. Solution Chemistry
4
A. Histological Solutions
B. Terminology
C. Preparation Principles
VI. Tissue Fixation
6
A. Fixing Fluids
B. Methods and Appropriate Selection
C. Washing, Dehydrating and Clearing Methods
2
COURSE TOPICS (Continued)
VII. Embedding Methods
CONTACT HOURS
__PER TOPIC__
2
A. Embedding Media
B. Types of Embedding
C. Embedding Procedure
VIII. Sectioning
8
A. Equipment
1. Technics
2. Microtome Operation
B. Types of Sectioning
1. Frozen
2. Paraffin
3. Nitrocellulose
IX. Mounting and Covering
1
A. Mounting Techniques for Section Types
B. Covering Techniques for Section Types
X. Staining
A.
B.
C.
D.
8
Types
Preparation - Routine
General Procedure
Routine Stain Preparation
XI. Introduction to Histochemistry
4
A. Inorganic Constituents of Tissue
B. Organic Constituents of Tissue
1. Polysaccharides
2. Proteins and Amino Acids
3. Lipids
4. Enzymes
XII. Quality Assurance in Histology Laboratory
6
A. Purpose
B. Components
C. Design
3
PROGRAM TITLE:
Histologic Technology
COURSE TITLE:
Histotechniques I
CIP NUMBER:
1351100800
Program Frameworks can be found at the following website: http://www.fldoe.org/workforce/dwdframe/
4
Florida State College at Jacksonville
Course Learning Outcomes and Assessment
SECTION 1
Semester Credit Hours (Credit):
Contact Hours (Workforce):
Course Prefix and Number:
MLT 2191
Course Title:
Histotechniques I
4
SECTION 2a (To be completed for General Education courses only.)
TYPE OF COURSE (Place an “X” in the box next to those that are applicable.)
General Education Core (If selected, core discipline area will be identified in Section 4.)
General Education (If selected, you must also complete Section 4, Section 5, and Section 8)
SECTION 2b
TYPE OF COURSE (Place an “X” in the box next to those that are applicable.)
A.A. Elective
X A.S. Required Course
A.S. Professional Elective
A.A.S. Required Course
A.A.S. Professional Elective
Technical Certificate
PSAV/Clock Hour/Workforce
Development Education
Apprenticeship
Upper Division/Bachelors
Other:
SECTION 3
INTELLECTUAL COMPETENCIES (Place an “X” in the box next to those that are applicable.)
X
Reading
X
Speaking
X
Critical Analysis
X
Qualitative Skills
X
Writing
X
Listening
X
Information
Literacy
X
Ethical Judgement
X
Scientific Method of
Inquiry
Working
Collaboratively
SECTION 4 (To be completed for General Education courses only.)
GENERAL EDUCATION DISCIPLINE AREA (Place an “X” in the box next to those that are applicable.)
Communications
Humanities
Mathematics
Social and Behavioral Sciences
Natural Sciences
SECTION 5 (To be completed for General Education courses only.)
GENERAL EDUCATION LEARNING OUTCOME AREA (Place an “X” in the box next to those that are applicable.)
Communication
Critical Thinking
Information Literacy
Scientific and Quantitative Reasoning
Global Sociocultural Responsibility
SECTION 6
LEARNING OUTCOMES
Demonstrate ability to apply mathematical
formulas to problems involving the
preparation of histology reagents.
Apply knowledge of tissues (gross &
microscopic features) to problems in special
fixation, processing, and embedding
techniques.
Compare and contrast routine microtomy to
frozen sectioning, to include purpose, type of
specimen, handling considerations,
biohazards, and staining.
TYPE OF OUTCOME
(General Education,
Course or Program)
METHOD OF ASSESSMENT
Program
Online quizzes, Midterm exam, & final exam.
Program
Discussion forum, online quizzes, Midterm
exam, & final exam.
Program
Online quizzes, Midterm exam, & final exam.
5
SECTION 6 (Continued)
LEARNING OUTCOMES
Discuss a routine histology laboratory’s
repertoire of special stains, with respect to
purpose of the stain and principle of staining
reaction.
Apply basic concepts of immunology to the
use of immunohistochemical reactions.
TYPE OF OUTCOME
(General Education,
Course or Program)
METHOD OF ASSESSMENT
Program
Online quizzes, Midterm exam, & final exam.
Program
Online quizzes, Midterm exam, & final exam.
SECTION 7
Faculty name(s):
Jerry Santiago
Date:
1-26-2015
CS20150615
6