Download microorganisms

THE USE OF BENEFICIAL
MICROORGANISMS IN CLEANING
FORMULATIONS
2
 Microorganisms overview
 Microorganisms and chemistry
 What can you do with microorganisms?
 Focus on drain application
 Regulatory
Agenda
MICROORGANISMS OVERVIEW
4
MICROORGANISMS ARE LIVING CREATURES
FOUND EVERYWHERE ON EARTH…
Only 10% of the
cells in the human
body are human!
Earth
Ocean
Soil
Human Body
5 nonillion
(5×1030)
10 million
in a ml. of water
40 million
in a gram of soil
40 million in a ml. of saliva
100 trillion in the gut flora
10% of total dry weight
5
THE VAST MAJORITY OF MICROORGANISMS
ARE EXTREMELY SAFE
In fact, we not only live with microbes,
but our health is dependent on them
(gut bacteria, immune system development, etc)
6
WHAT ARE BACTERIA?
0.5 – 3µm
Bacteria are organisms that are:
• Living
• Single-celled
• Microscopic
7
BACTERIAL NUTRITION/
ENVIRONMENTAL CONDITIONS
• Carbon, Nitrogen, Phosphorus and some trace of
minerals to grow
• Moisture, proper temperature, appropriate pH and
something to “breathe” (an electron acceptor like
O2, NO3, etc.)
• Production of enzymes (biocatalysts) that break
down compounds like fat, starch and protein to
yield these essential nutrients
8
COMPARISON OF BACTERIA AND ENZYMES
Raw enzymes are...
• fast starting biochemicals
• specific for a certain reaction
• proteins that can be degraded by other enzymes
Bacteria...
• take a bit longer awaken and to show activity
• produce enzymes on demand, in response to
compounds present (in situ enzyme factories)
• can produce suite of enzymes to completely
degrade a compound
• can use the components and energy from the
enzymatic action to produce more bacteria
9
BACTERIA PRODUCE ENZYMES
IN PART, ENZYMES ARE USED TO FEED THE
BACTERIA
Extracellular enzymes
Large molecules,
particulate or
colloidal material
Small,
soluble
compounds
Intracellular
enzymes
CO2 & H2O
Only ~3% of enzymes used by microorganisms are secreted;
the rest are accessible for useful applications only by using the whole cell
MICROORGANISMS AND
CHEMISTRY
11
CHEMISTRY/ENZYMES/MICROORGANISMS
SURFACTANT/
SOLVENT
• Cleaning by
displacement and
solubilization of
organic soils
• Increase the
efficiency of enzymes
and microorganisms
by contact
improvement
ENZYMES
• Cut organic soils
/catalyze chemical
reaction
• Facilitate the rollingup and cleaning
(surfactant,
mechanical action…)
MICROORGANISMS
• Enzyme factory
• Metabolize/
degrade organic
substrates
• Minimize impact on
the environment
+ H2O
+ CO2
Same organic
content
Same organic
content
Organic content
decreased
12
FORMULATE WITH MICROORGANISMS
pH: 5.0 to 9.0 for optimal
stability in liquids
Easy to formulate but need basic expertise (living technology)
SO, WHAT CAN YOU DO WITH
MICROORGANISMS?
14
MICROORGANISMS BENEFITS
 Short term cleaning: chemistry and enzymes
 Mid-term and long term degradation: microorganisms
Microorganisms
Chemistry
Continuous organic degradation
(fat, starch, protein, VFA)
Enzymes
Fight stains
0
< 1 min
1 hour
Odor control
Time
15
CURRENT CLEANING SOLUTIONS APPLICATIONS
Carpet & fabric
Drain
Grease Interceptor
• Improved cleaning
• Decrease resoiling
• Avoid blockages
• Odor control
• Odor control
• Prevent grease release in
the sewer
Hard surface
Odor control
Septic & waste
• Deep cleaning
• Odor control
• Drain
• Hard surface
• Grease interceptor
• Septic & waste
• Odor control
• Reduce blockages
• Enhance the degradation
FOCUS ON DRAIN APPLICATION
17
KITCHEN AND BATHROOM SOIL CLEANING
Effective soil cleaning
Green Worx’s technology
effectively removes both
bathroom soil (left) and kitchen
soil (right) applied to ceramic
tiles.
Before
treatment
with Green
Worx's
technology
Water rinse
only
Treated with
Green
Worx's
technology
18
KITCHEN SOIL CLEANING
Green Worx CSs’
technology effectively
removes kitchen soil
(right) while the control
without treatment (left)
shows no change
19
KITCHEN SOIL CLEANING
Untreated
(kitchen soil only)
Treated with GW
microorganisms
Free flowing drain line
The tube treated with Green Worx’ microorganisms
(right) shows almost complete soil removal, and the
time for water to flow through the tube improved by 25
seconds.
20
MICROBIAL ACTIVITY ON KITCHEN SOIL
Respirometric activity
The long-lasting,
continuous degradation
(15+ days) of kitchen
soil and grease is
demonstrated by the
respirometric activity of
Green Worx’ beneficial
microorganisms.
21
PROTEIN DEGRADATION
After 7 days of treatment with Green Worx s’ beneficial microorganisms, a
black protein cube is degraded and black dye is released (right) whereas the
control (left) shows no protein degradation.
22
STARCH DEGRADATION
Complex starch degradation before (left) and 72 hours after (right) addition of
Green Worxs’ beneficial microorganisms.
23
ODOR CONTROL - MICROBIAL GROWTH ON
ODOROUS MOLECULES AND BUILDUP
Respirometry test on VFA
Green Worxs’ beneficial
microorganisms can
degrade both the longand the short-chain fatty
acids commonly found in
kitchen drain soil and
responsible for malodors
and buildup.
REGULATORY
25
REGULATORY AND SAFETY INFORMATION
• Tight quality control and stringent tests are incorporated
into the production process to ensure that Green Worx CS
products are manufactured to the highest quality standards
• All microorganisms are isolates of naturally occurring
organisms and are not genetically engineered or
modified
• All microorganisms have been identified by 16s rDNA
sequencing and confirmed to belong to Risk Group 1 as
defined by the Directive 2000/54/EC.
• Safety assessment performed on each Green Worx CS
product prior to market
• Reach compliance of all raw materials is checked
.
26
CONCLUSION
• Beneficial bacteria can degrade overtime, making preventive
treatment really efficient
• Spore-forming beneficial bacteria have long shelf life
• Bacteria are sustainable and less toxic than traditional chemical
control agents
• Existing of a large collection of bacteria-based products used
for degradation of grease, oil, odors in kitchen drains and
carpets, organic soiling/stains, etc.
• Green Worx CSs’ beneficial microorganisms could offer safe,
sustainable, efficient solution to any application where control of
undesirable compounds is needed
THANKS FOR
YOUR ATTENTION!
QUESTIONS?