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Why Divide? The cell theory states that: All cells come from other living cells. 1. Cells divide for the survival of the individual organism 2. Cells divide for the survival of the species (group of similar organisms) Cells divide for the survival of the individual organism Cell division is used for growth, development, maintenance, and repair of the organism. This is carried out by: • Mitosis- division of body cells to make more of the same kind of cell. – – • The parent cell is the original cell inside the organism that will divide (i.e. a skin cell) The daughter cells are the two identical cells created when the parent cell divides/replicates Also a form of asexual reproduction BASIC GENETICS • Each cell in the human body contains two sets of 23 chromosomes (contains DNA/genetic material) • Mitosis identically replicates this information No genetic modifications! • Each cell in the body therefore has the same/identical genetic material • Reproductive cells only have one set of chromosomes which combine to make a new person with different genetic material to both parents This is NOT through mitosis!! Each cell has 23 different chromosomes. They’re arranged in pairs since 1 chromosome of the pair comes from the mom, the other from the dad. This makes a total of 46 chromosomes. M = maternal (mom) M P M P P = paternal (dad) “Pa” P M 1. Chromosome: coiled DNA structures within the nucleus containing genetic information that is passed on from generation to generation 2. Sister Chromatid: one of two identical “sister” parts of a duplicated chromosome 3. Centromere (A): chromosome region that joins 2 sister chromatids Sister chromatids TEM 36,000 Centromere Cell Division: Mitosis • Mitosis – “division of the nucleus”. It is a synchronized set of events that will separate chromosomes, so that the two nuclei formed will be identical. • It is the beginning of cellular division. • (PMAT)– Prophase, Metaphase, Anaphase, & Telophase • Mitosis Animation Simple Stages of Cell Division Interphase - Getting ready for cell division Mitosis - Prophase - Metaphase - Anaphase - Telophase Cytokinesis -Splitting cytoplasm after mitosis -Cell growth - DNA Replicates (doubles the amount of genetic material) - Cell prepares for division - Nuclear membrane breaks down - DNA coils to appear as chromosomes - Spindle fibers attach to chromosomes - Centrioles separate to opposite poles - Chromosomes line up in middle of cell -Centromere of each double stranded chromosomes attach to spindle fibers - Centromeres divide and then separate -Spindle fibers shorten pulling chromatids apart to opposite sides of cell - Chromosomes uncoil -Nuclear membrane forms around each new set of chromosomes - Cytokinesis begins Cytokinesis 1. Cytokinesis is division of the cytoplasm 2. Distributes the organelles a. In animal cells, the cell membrane pinches in two. b. In plant cells, a cell plate forms across the middle. What Phase Of the Cell Cycle Is It? A Anaphase D Interphase B C Telophase Cytokinesis E F Metaphase Prophase Figure 12.0 Mitosis Figure 12.4 The cell cycle Uses of mitosis: Asexual Reproductions Growth Repair Difference Between Plant and Animal Mitosis Animals have centrioles, Plants cells usually do not Animal cells divide by pinching in (Cleavage), Plant cells divide by forming a cell plate. Cell plate forming Cleavage furrow Daughter nucleus Contracting ring of microfilaments Daughter cells TEM 7,500 SEM 140 Cleavage furrow Wall of parent cell Cell wall Vesicles containing cell wall material New cell wall Cell plate Daughter cells CANCER • is the caused by uncontrolled cellular division. • Typically, most cells don’t divide unless it is for repair or maintenance. • Cancerous cells keep dividing; crowding out and stealing nutrients from healthy tissue. • Cancer “kills” when a mass of cancer cells called a tumor interferes with the functions of major organs. Breast Cancer (uncontrolled cell growth) Lymph vessels Tumor Blood vessel Glandular tissue A tumor grows from a single cancer cell. Cancer cells invade neighboring tissue. Cancer cells spread through lymph and blood vessels to other parts of the body. Cells divide for the survival of the species - One parent -Offspring genetically identical to parent cell -will have same #chromosomes parent cell -will have all same characteristics as parent cell - MITOTIC CELL DIVISION - cytoplasm and organelles split equally Example: amoeba, paramecium, bacteria -single circular chromosome replicates before division Unicellular: Uneven division of cytoplasm and organelles (ex. Yeast) Multicellular: -A cell of a parent develops a multicellular bud - eventually bud separates becoming separate organism (ex. Hydra) -Mitosis with in specialized cells producing spores. -spore is DNA surrounded by a tough coat (survive harsh environment) - each spore can develop into a new organism Example: Bread Mold - Replacement of lost or damaged body parts -Invertebrates show greater regenerative capacity than vertebrates - examples: lobster, starfish, planaria In some cases part can grow a new organism New plant develops from part of a parent plant Bulbs: -Ex. Onion, Tulip Tubers: -Ex. Potato Runners: -stem above the ground -new plants form where runners touches ground (Ex. Strawberries, spider plant) Rhizomes: -woody underground stem -swollen regions produce new plant (Ex. Poison Ivy, Ferns) 2n = diploid (normal chromosome # for species) 46 Single 2n Chromosomes Interphase 46 Double 2n Chromosomes 46 2n singles P M A T (Mitosis) 46 Cytokinesis 2n singles Everyone writes statement/answer in notebook Partner A answers: #1, Partner B answers: #2 etc. 1 1. # nuclear divisions: 2. Chromosome # (identical or different from parent cell) Identical 3. # daughter cells created 2 4. Genetic information (identical or different from parent) Identical 5. Uses Growth, repair, asexual reproduction 6. When are chromosomes single, double? Single=before interphase, after telophase Double= after interphase, until telophase What are clones? - Genetically identical copies i.e. have the same traits Hydra budding to make a clone LM 340 Amoeba dividing asexually, making a clone Bacteria dividing asexually • These are CLONES as only have information from 1 parent Strawberry plants making asexual clones • Whole new plants grow at the end of the runners