Download Syllabus Cambridge International A & AS Level Chemistry Syllabus code 9701

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4. Syllabus content
11.2 Applications of analytical chemistry
Questions in this section will focus on the techniques involved rather than the recall of examples. All
necessary information about the context will be given in the question and candidates will be expected to
apply their knowledge.
Learning outcomes
Methods of detection and
Candidates should be able to:
Applications in chemistry
and society
(a) describe simply the process of electrophoresis and the effect of
pH, using peptides and amino acids as examples (link to 11.1
(b) explain, in simple terms, the technique of DNA fingerprinting
and its applications in forensic science, archaeology and
(c) describe the importance to modern medicine, and the
challenges, of separating and characterising the proteins in
cells (link to 11.1)
(d) outline in simple terms the principles of nuclear magnetic
resonance in 1H and be able to interpret simple NMR spectra
(e) show awareness of the use of NMR and X-ray crystallography
in determining the structure of macromolecules and in
understanding their function (link to 11.1 (c) and 11.2 (c))
(f) state what is meant by partition coefficient and calculate a
partition coefficient for a system in which the solute is in the
same molecular state in the two solvents
(g) understand qualitatively paper, high performance liquid, thin
layer and gas/liquid chromatography in terms of adsorption
and/or partition and be able to interpret data from these
(h) explain the concept of mass spectroscopy, deduce the
number of carbon atoms in a compound using the M+1 peak
and the presence of bromine and chlorine atoms using the
M+2 peak and suggest the identity of molecules formed by
simple fragmentation in a given mass spectrum (see also core
syllabus, section 1 (c) and (d))
(i) draw conclusions given appropriate information and data
from environmental monitoring (for example, PCBs in the
atmosphere, isotopic ratios in ice cores)
Cambridge International A & AS Level Chemistry 9701. Examination in June and November 2012.