Download Section 3 Teacher Notes

Biology
Biology
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12–3 RNA and Protein Synthesis
12-3 RNA and Protein Synthesis
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12–3 RNA and Protein Synthesis
12–3 RNA and Protein
Synthesis
Genes are coded DNA instructions that control the
production of proteins.
Genetic messages can be decoded by copying part
of the nucleotide sequence from DNA into RNA.
RNA contains coded information for making proteins.
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12–3 RNA and Protein Synthesis
The Structure of RNA
The Structure of RNA
RNA consists of a long chain of nucleotides.
Each nucleotide is made up of a 5-carbon sugar, a
phosphate group, and a nitrogenous base.
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12–3 RNA and Protein Synthesis
The Structure of RNA
There are three main differences between RNA and
DNA:
• The sugar in RNA is ribose instead of
deoxyribose.
• RNA is generally single-stranded.
• RNA contains uracil in place of thymine.
Whoa! U instead of T!!
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Types of RNA
What are the three main types of RNA?
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Types of RNA
Types of RNA
There are three main types of RNA:
• messenger RNA
• ribosomal RNA
• transfer RNA
(mRNA)
(rRNA)
(tRNA)
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Types of RNA
Messenger RNA (mRNA) carries copies of
instructions for assembling amino acids into proteins.
This is the negative /blueprint of our
“master plan”
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Types of RNA
Ribosome
Ribosomal RNA
Ribosomes are made up of proteins and ribosomal RNA
(rRNA).
This is our secret decoder ring
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Types of RNA
Amino acid
Transfer RNA
During protein construction, transfer RNA (tRNA)
transfers each amino acid to the ribosome.
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12–3 RNA and Protein Synthesis
Transcription
What is transcription?
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Transcription
Transcription
RNA molecules are produced by copying part of a
nucleotide sequence of DNA into a complementary
sequence in RNA. This process is called
transcription.
Transcription requires the enzyme RNA
polymerase.
What does it mean to transcribe something?
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Transcription
During transcription, RNA polymerase
binds to DNA and separates the DNA
strands.
RNA polymerase then uses one strand
of DNA as a template from which
nucleotides are assembled into a strand
of RNA.
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Transcription
RNA polymerase binds only to regions of DNA known
as promoters.
Promoters are signals in DNA that indicate to the
enzyme where to bind to make RNA.
Promoters are the starting point of transcription.
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12–3 RNA and Protein Synthesis
Transcription
RNA
RNA polymerase
DNA
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12–3 RNA and Protein Synthesis
RNA Editing
RNA Editing
The DNA of eukaryotic genes contains sequences
of nucleotides, called introns, that are not involved
in coding for proteins.
The DNA sequences that code for proteins are
called exons. (because their information is
expresed as proteins).
When RNA molecules are formed, introns and
exons are copied from DNA.
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12–3 RNA and Protein Synthesis
The introns are cut
out of RNA molecules.
RNA Editing
Exon Intron
The exons are the
spliced together to
form mRNA
DNA
Pre-mRNA
We are editing out the
“nonsense” from the
RNA.
mRNA
Cap
Tail
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12–3 RNA and Protein Synthesis
The Genetic Code
The Genetic Code
The genetic code is the “language” of mRNA
instructions.
The code is written using four “letters” (the bases:
A, U, C, and G).
Whoa! U is used instead of T !!!!!
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The Genetic Code
A codon consists of three consecutive nucleotides
on mRNA that specify a particular amino acid.
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12–3 RNA and Protein Synthesis
The Genetic Code
Each codon specifies a particular amino acid that
is to be placed on the polypeptide chain.
Some amino acids can be specified by more than
one codon.
Redundant
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The Genetic Code
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12–3 RNA and Protein Synthesis
The Genetic Code
There is one codon AUG that can either specify
the amino acid methionine or serve as a “start”
codon for protein synthesis.
There are three “stop” codons that do not code for
any amino acid. These “stop” codons signify the
end of a polypeptide.
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12–3 RNA and Protein Synthesis
Translation
What is translation?
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Translation
Translation
Translation is the decoding of an mRNA
message into a polypeptide chain (protein).
Translation takes place on ribosomes.
During translation, the cell uses
information from messenger RNA to
produce proteins.
Do not confuse this with
Transcription!
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Translation
Messenger RNA is
transcribed in the nucleus,
and then enters the cytoplasm
where it attaches to a
ribosome.
Nucleus
mRNA
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Translation
Translation begins when an
mRNA molecule attaches to a
ribosome.
As each codon of the mRNA
molecule moves through the
ribosome, the proper amino
acid is brought into the
ribosome by tRNA.
In the ribosome, the amino
acid is transferred to the
growing polypeptide chain.
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Translation
Each tRNA molecule carries only one kind of amino
acid.
In addition to an amino acid, each tRNA molecule
has three unpaired bases.
These bases, called the anticodon, are
complementary to one mRNA codon.
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Translation
The ribosome binds new tRNA molecules and amino
acids as it moves along the mRNA.
Phenylalanine
tRNA
Lysine
Methionine
Ribosome
mRNA
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Start codon
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12–3 RNA and Protein Synthesis
Translation
Protein Synthesis
Lysine
tRNA
Translation direction
mRNA
Ribosome
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Translation
The process continues until the ribosome reaches
a stop codon.
Polypeptide
Ribosome
tRNA
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mRNA
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12–3 RNA and Protein Synthesis
The Roles of RNA and DNA
The Roles of RNA and
DNA
The cell uses the DNA
“master plan” to prepare
RNA “blueprints.” The DNA
stays in the nucleus.
The RNA molecules go to
the protein building sites in
the cytoplasm—the
ribosomes.
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12–3 RNA and Protein Synthesis
Genes and Proteins
Genes and Proteins
Genes contain instructions for assembling
proteins.
Many proteins are enzymes, which catalyze and
regulate chemical reactions.
Proteins are each specifically designed to build or
operate a component of a living cell.
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Codon
The sequence of
bases in DNA is used
as a template for
mRNA.
The codons of mRNA
specify the sequence
of amino acids in a
protein.
Way cool transcription/translation video:
Genes and Proteins
Codon Codon
Single strand of DNA
Codon Codon Codon
mRNA
Alanine Arginine Leucine
http://www.youtube.com/watch?
v=41_Ne5mS2ls
Amino acids within
a polypeptide
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Click to Launch:
Continue to:
- or -
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The role of a master plan in a building is similar
to the role of which molecule?
a. messenger RNA
b. DNA
c. transfer RNA
d. ribosomal RNA
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A base that is present in RNA but NOT in DNA is
a. thymine.
b. uracil.
c. cytosine.
d. adenine.
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The nucleic acid responsible for bringing
individual amino acids to the ribosome is
a. transfer RNA.
b. DNA.
c. messenger RNA.
d. ribosomal RNA.
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A region of a DNA molecule that indicates to an
enzyme where to bind to make RNA is the
a. intron.
b. exon.
c. promoter.
d. codon.
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A codon typically carries sufficient information to
specify a(an)
a. single base pair in RNA.
b. single amino acid.
c. entire protein.
d. single base pair in DNA.
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