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Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Lab 1 Notes There are 3 bacterial forms 1. Cocci: Spherical (round) 2. Bacillus: rod shaped 3. Spirillus: Spiral • Bacillus: rod shaped • is a gram-negative bacteria • • Anthrax is caused by Bacillus anthracis. Bacilli are resistant to heat, cold, radiation, desiccation, and disinfectants There are 3 bacterial forms - Bacillus: rod shaped ENDOSPORES • Bacillus: rod shaped • • • Are extremely resilient due to having endospores An endospore is a dormant, tough, and non-reproductive structure produced by certain bacteria Endospores enable bacteria to lie dormant for extended periods, even centuries. Revival of spores millions of years old has been claimed. There are 3 bacterial forms - Cocci: round • Cocci: Spherical (round) • Staphylococcus aureus Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi • • causes a variety of infections in the body, including boils, cellulitis, abscesses, wound infections, toxic shock syndrome, pneumonia, and food poisoning. Streptococcal bacteria • cause a variety of infections in the body, including pneumonia, meningitis, ear infections, and strep throat. There are 3 bacterial forms - Spirillum: (spiral) • Spirillum: (spiral) All are aquatic except for one species (S. minus) that causes a type of rat-bite fever in man. Spirillum is a gram-negative, motile helical cell with flagella at each end. There are 3 bacterial forms - Bacillus: rod shaped • Spirillum: (spiral) Spirochetes – Some of these cause Lyme disease Syphillis Cyanobacteria Cyanobacteria, is a phylum of bacteria that obtain their energy through photosynthesis. The name "cyanobacteria" comes from the color of the bacteria (Greek: κυανός (kyanós) = blue). They are often called blue-green algae, although the name is sometimes considered a misnomer Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi because cyanobacteria are prokaryotes and the term "algae" is often reserved for eukaryotes. Are considered gram-negative bacteria. Cyanobacteria How did cyanobacteria contribute to life on Earth? Many Proterozoic oil deposits are attributed to the activity of cyanobacteria. They are also important providers of nitrogen fertilizer in the cultivation of rice and beans. The other great contribution of the cyanobacteria is that they were the precursors to plants. They changed the early atmosphere through photosynthesis. Chloroplasts in plants is a symbiotic cyanobacterium. Bacterial Diseases - Tuberculosis Tuberculosis, caused by the bacterium Mycobacterium tuberculosis, which kills about 2 million people a year, mostly in sub-Saharan Africa. Pathogenic bacteria contribute to other globally important diseases, such as pneumonia, which can be caused by bacteria such as Streptococcus and Pseudomonas, and foodborne illnesses, which can be caused by bacteria such as Shigella, Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Campylobacter, and Salmonella. Pathogenic bacteria also cause infections such as tetanus, typhoid fever, diphtheria, syphilis, and leprosy. Bacterial Diseases - Tetanus Top 10 Bacterial Infections Tetanus is caused by the bacterium Clostridium tetani. It enters the body via an open wound and releases a powerful toxin, tetanospasmin. The incubation period lasts from 2 days to several weeks. This infection causes fever, pain, spasms of the neck and jaws. The treatment includes sedation, administration of muscle relaxing chemicals, antibiotics and antitoxins. The vaccine confers immunity for 5 years; in the case of profound wounds, injections with antitoxins are required. Read more: http://news.softpedia.com/news/Top-10-Bacterial-Infections83326.shtml#ixzz4IV3hfbql Bacterial Diseases - Typhoid fever Top 10 Bacterial Infections Typhoid fever is caused by the bacterium Salmonella enterica. The sources of infection are represented by contaminated water and food. The incubation lasts 7 to 14 days, then fever, headaches, constipation and diarrhea install. The treatment consists of antibiotics. To avoid this infection, food must be processed and manipulated in hygienic conditions. The vaccine confers limited immunity. This disease usually accompanies wars. A huge typhus pandemic broke Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi out during the First World War in the Eastern Europe. Since 1914, over 20 million people died of typhus. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV3pKZ4P Bacterial Diseases - Cholera Top 10 Bacterial Infections Cholera is caused by the bacterium Vibrio cholerae. Humans take the bacterium from water infested with human feces Causes chronic diarrhea, dehydration, loss of liquids and salts. These losses must be replaced. The vaccine confers limited protection, that's why hygiene is the main method of controlling cholera. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV3xK7TS Bacterial Diseases - black plague Top 10 Bacterial Infections Plague is caused by Yersinia pestis. The black plague broke out in Europe in 1347, when a boat coming from Crimea docked at Mesina, Sicily. Besides its load, the ship transported the pest, which soon Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi spread throughout Italy. It was like the end of the days for Europe. In four years, this bacterium killed 20 to 30 million Europeans, about one third of the continent's population. Even the remote Iceland was struck. In the Extreme East, China dwindled from 123 million inhabitants at the beginning of the 13th century to just 65 million during the 14th century, because of the pest and hunger. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV4iBIzU Bacterial Diseases - black plague Top 10 Bacterial Infections Plague is caused by Yersinia pestis. The black plague broke out in Europe in 1347, when a boat coming from Crimea docked at Mesina, Sicily. Besides its load, the ship transported the pest, which soon spread throughout Italy. It was like the end of the days for Europe. In four years, this bacterium killed 20 to 30 million Europeans, about one third of the continent's population. Even the remote Iceland was struck. In the Extreme East, China dwindled from 123 million inhabitants at the beginning of the 13th century to just 65 million during the 14th Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi century, because of the pest and hunger. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV4iBIzU Bacterial Diseases - black plague Top 10 Bacterial Infections The pest bacterium is transmitted by fleas and, usually, the infection jumps from rats to humans. The incubation lasts 2 to 10 days. The disease causes fever, swelling of the lymphatic ganglions and skin. Today, antibiotics can treat plague. The vaccine confers limited immunity. This catastrophe has no match in the human history. 25 to 50 % of the inhabitants of Europe, North Africa and certain Asian areas died back then. Knowing the cause of the pandemic helped: in 1907, an outbreak of bubonic plague in San Francisco produced just several victims, as the authorities started a massive campaign for exterminating the rats, while in 1896 an outbreak in India caused 10 million deaths in 12 years, as the cause was not known. Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV4thanK Bacterial Diseases - Syphilis Top 10 Bacterial Infections Syphilis, caused by the bacterium Treponema pallida, is the most severe sexually transmitted bacterial infection. The first stage has an incubation of 3-12 weeks and it induces ulcered lesions (syphilis chancre) at the entrance of body's aperture organ. After that, it triggers skin eruptions, fever, hair loss, less severe hepatitis and genital condilloma, but if untreated, the lesions extend in several years to the nervous system, leading to death. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV53CMh5 Bacterial Diseases - Syphilis Top 10 Bacterial Infections The treatment consists in extremely powerful antibiotics (ceftriaxone, Cefixime, and others) which are also extremely costly. Antibiotics are most effective in the first stages. People must avoid Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi having sex with probable carriers of the infection; it requires immediate treatment, ceasing sexual contacts until the end of the treatment and informing of the recent sexual contacts, for medical control and treatment. Bacterial Diseases - Syphilis Top 10 Bacterial Infections Gonorrhea is triggered by the Neisseria bacteria and it is transmitted sexually. 62 million people, aged mainly 15 to 29, are affected worldwide, especially in urban areas and of low socioeconomic level. The incubation lasts 3 days, and in men, gonorrhea produces urinary incontinence, urethra pain, reddening, penis burning sensation and testicle inflammation. In women, it induces severe pain that reaches the trumps and uterus. The treatment uses antibiotics and prevention is similar to syphilis. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV5OhUZv Bacterial Diseases - Tuberculosis Top 10 Bacterial Infections Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Tuberculosis is caused by the Koch bacterium (Mycobacterium tuberculosis). It is as old as the humankind is. TBC was found even in mummies coming from the ancient Egypt and Peru. 2 million people die annually of tuberculosis. About 150 million people are estimated to have died of TBC since 1914. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections83326.shtml#ixzz4IV5bpeBW Bacterial Diseases - Tuberculosis Top 10 Bacterial Infections One third of the people carry the Koch bacterium, which spreads through the air and milk from infested cows and affects all the body, especially the lungs. It induces prolonged coughing, fever, shivering, bloody expectoration, weight loss, sweating, tiredness, and glossy eyes. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV5thJ3W Bacterial Diseases - Tuberculosis Top 10 Bacterial Infections Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi It infects one third of the world population and each year another new 8 million cases appear. Each second a person dies of tuberculosis. It is more aggressive in women and persons between 15 and 45 years old. Mutant strains are resistant to almost all drugs and kill about 50 % of the patients. It is spread worldwide, but its advance is rampant in Bangladesh, China, Indonesia, Philippines, India and Pakistan, with over half of the new cases. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV69C2nW Bacterial Diseases - Legionnaire's disease Top 10 Bacterial Infections Legionnaire's disease is caused by Legionella bacteria. The bacteria are taken from air or wet environments. It causes symptoms similar to flue or pneumonia, accompanied by renal failure. The disease requires hospitalization and treatment with antibiotics. As a prevention measure, water and air conditioning installation must be controlled. Read more: http://news.softpedia.com/news/Top10-Bacterial-Infections-83326.shtml#ixzz4IV6HzzWv Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Bacterial Diseases - Pneumonia Top 10 Bacterial Infections Pneumonia affects 1% of the planet's population and can be produced by bacteria (like Aeromonas hydrophila) or viruses. It produces fever, shiver, sweating, cough with expectoration, muscle, head and thoracic pain, appetite loss, weakness. This is the main cause of mortality in the world: it kills 3.5 million people each year. It attacks especially Antibiotics They may either kill or inhibit the growth of bacteria. Fleming was working on a culture of diseasecausing bacteria when he noticed the spores of a little green mold (Penicillium chrysogenum), in one of his culture plates. He observed that the presence of the mold killed or prevented the growth of the bacteria.[6] Antibiotics They may either kill or inhibit the growth of bacteria. Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Fleming was working on a culture of diseasecausing bacteria when he noticed the spores of a little green mold (Penicillium chrysogenum), in one of his culture plates. He observed that the presence of the mold killed or prevented the growth of the bacteria.[6] Antibiotics They are produced in nature by soil bacteria and fungi. This gives the microbe an advantage when competing for food and water and other limited resources in a particular habitat, as the antibiotic kills off their competition. Title and Content Layout with Chart Areas of YOUR body that are more susceptible to infection Ear Infection Lung Infection Throat Infection Yeast Infection Urinary Tract Infection H. Pylori – Ulcers Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Good bacteria In the human gastrointestinal tract, good bacteria aid in digestion and produce vitamins. Good bacteria assists the body’s immunity, making the body less hospitable to bad bacteria and other harmful pathogens. When considering all the strains of bacteria that exist, relatively few are capable of making people sick. Good bacteria is used to make some of the foods we enjoy. Acidophilus milk is made with Lactobacillus acidophilus. Butter is made from pasteurized cream, to which a lactic acid starter has been added. Cheese is often made with Streptococcus and Lactobacillus bacteria. Yogurt usually requires the addition of Lactobacillus bulgaricus, Lactococcus thermophilus, and/or Streptococcus thermophilus to the milk. Beers, etc. -- Traditionally, the natural yeasts on grape skins determine the quality of wine produced. Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi These natural yeasts, especially Saccharomyces cerevisiae (beer in Spanish is "cervesa") and Saccharomyces ellipsoideus, ferment the grapes to make wine. Bacteria have a wide range of envronmental and nutritive requirements. Most bacteria may be placed into one of three groups based on their response to gaseous oxygen. Aerobic bacteria thrive in the presence of oxygen and require it for their continued growth and existence. Other bacteria are anaerobic, and cannot tolerate gaseous oxygen, such as those bacteria which live in deep underwater sediments, or those which cause bacterial food poisoning. The third group are the facultative anaerobes, which prefer growing in the presence of oxygen, but can continue to grow without it. Bacteria may also be classified both by the mode by which they obtain their energy. Classified by the source of their energy, bacteria fall into two categories: heterotrophs and autotrophs.Heterotrophs derive energy from Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi breaking down complex organic compounds that they must take in from the environment -- this includes saprobic bacteria found in decaying material, as well as those that rely on fermentation or respiration. The other group, the autotrophs, fix carbon dioxide to make their own food source; this may be fueled by light energy (photoautotrophic), or by oxidation of nitrogen, sulfur, or other elements (chemoautotrophic). While chemoautotrophs are uncommon, photoautotrophs are common and quite diverse. They include the cyanobacteria, green sulfur bacteria, purple sulfur bacteria, and purple nonsulfur bacteria. The sulfur bacteria are particularly interesting, since they use hydrogen sulfide as hydrogen donor, instead of water like most other photosynthetic organisms, including cyanobacteria. Characterization of bacteria Gram negative / gram positive Aerobic / anaerobic Heterotrophs / autotrophs Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Bacteria play important roles in the global ecosystem. The ecosystem, both on land and in the water, depends heavily upon the activity of bacteria. The cycling of nutrients such as carbon, nitrogen, and sulfur is completed by their ceaseless labor. Organic carbon, in the form of dead and rotting organisms, would quickly deplete the carbon dioxide in the atmosphere if not for the activity of decomposers. This may not sound too bad to you, but realize that without carbon dioxide, there would be no photosynthesis in plants, and no food. When organisms die, the carbon contained in their tissues becomes unavailble for most other living things. Decomposition is the breakdown of these organisms, and the release of nutrients back into the environment, and is one of the most important roles of the bacteria. Bacteria play important roles in the global ecosystem. The cycling of nitrogen is another important activity of bacteria. Plants rely on nitrogen from the soil for Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi their health and growth, and cannot acquire it from the gaseous nitrogen in the atmosphere. The primary way in which nitrogen becomes available to them is through nitrogen fixation by bacteria such as Rhizobium, and by cyanobacteria such as Anabaena, Nostoc, and Spirulina, shown at right. These bacteria convert gaseous nitrogen into nitrates or nitrites as part of their metabolism, and the resulting products are released into the environment. Some plants, such as liverworts, cycads, and legumes have taken special advantage of this process by modifying their structure to house the basteria in their own tissues. Other denitrifying bacteria metabolize in the reverse direction, turning nitrates into nitrogen gas or nitrous oxide. When colonies of these bacteria occur on croplands, they may deplete the soil nutrients, and make it difficult for crops to grow. Protists Major Categories of Protists Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Algae are simple plants that can range from the microscopic (microalgae), to large seaweeds (macroalgae), such as giant kelp more than one hundred feet in length. What are algae?? Algae (singular: alga) are plants or plantlike organisms that contain chlorophyll (pronounced KLOR-uh-fill) and other pigments (coloring matter) that trap light from the Sun. This light energy is then converted into food molecules in a process called photosynthesis. Most algae store energy as some form of carbohydrate (complex sugars). Most algae store energy as some form of carbohydrate (complex sugars). Uses of Algae as Energy source, Fertilizer, Food and Pollution control Humans use algae as food, for production of useful Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi compounds, as biofilters to remove nutrients and other pollutants from wastewaters, to assay water quality, as indicators of environmental change, in space technology, and as laboratory research systems. Algae is commercially cultivated for Pharmaceuticals, Nutraceuticals, Cosmetics and Aquaculture purpose. Algae – Feul Source Fuel source Algae can be used to make Biodiesel (see algaculture), Bioethanol and biobutanol and by some estimates can produce vastly superior amounts of vegetable oil, compared to terrestrial crops grown for the same purpose. Algae can be grown to produce hydrogen. In 1939 a German researcher named Hans Gaffron, while working at the University of Chicago, observed that the algae he was studying,Chlamydomonas reinhardtii (a green-algae), would sometimes switch from the production of oxygen to the production of hydrogen. Algae can be grown to produce biomass, which can be burned to produce heat and electricity. Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Algae – Food supplement: Food supplement: 1. It is a complete protein with essential amino acids (unlike most plant foods) that are involved in major metabolic processes such as energy and enzyme production. 2. It contains high amounts of simple and complex carbohydrates which provide the body with a source of additional fuel. In particular, the sulfated complex carbohydrates are thought to enhance the immune system’s regulatory response. 3. It contains an extensive fatty acid profile, including Omega 3 and Omega 6. These essential fatty acids also play a key role in the production of energy. 4. It has an abundance of vitamins, minerals, and trace elements in naturally-occurring synergistic design. Algae – Stabilizing agent Stabilizing agent Chondrus crispus, (probably confused Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi with Mastocarpus stellatus, common name: Irish moss), is also used as "carrageen". It is an excellent stabiliser in milk products - it reacts with the milk protein caesin, other products include: petfoods, toothpaste, ice-creams and lotions etc.,Alginates in creams and lotions are absorbable through the skin. Algae – Fertilizer Fertilizer Algae are used by humans in many ways. They are used as fertilizers, soil conditioners and are a source of livestock feed. Because many species are aquatic and microscopic, they are cultured in clear tanks or ponds and either harvested or used to treat effluents pumped through the ponds Algae – Role Of Algae in Pollution control Role Of Algae in Pollution control Algae are used in Wastewater Treatment facilities, reducing the need for greater amounts of toxic chemicals than are already used. Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Algae can be used to capture fertilizers in runoff from farms. When subsequently harvested, the enriched algae itself can be used as fertilizer. Algae Bioreactors are used by some powerplants to reduce CO2 emissions. The CO2 can be pumped into a pond, or some kind of tank, on which the algae feed. Alternatively, theBioreactor can be installed directly on top of a smokestack. wastewater treatment Using algae for wastewater treatment offers some interesting advantages over conventional wastewater treatment. The advantages of algaebased treatment include: Cost effective treatment Low energy requirement Reduction in sludge formation and Production of algal biomass Oil extraction from algae is a hotly debated topic currently because this process is one of the more Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi costly processes which can determine the sustainability of algae-based biodiesel. In terms of the concept, the idea is quite simple: Harvest the algae from its growth medium (using an appropriate separation process), and extract the oil out of it. Extraction can be broadly categorized into two methods: Amoeba Paramecium Trypanosoma Plasmodium is a genus of parasitic protozoa, many of which cause malaria in their hosts. Spread by mosquitos. Fungi A fungus is any member of the group of eukaryotic organisms that includes unicellular microorganisms such as yeasts and molds, as well as multicellular fungi that produce familiar fruiting forms known as mushrooms. Fungi - yeast Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi a microscopic fungus consisting of single oval cells that reproduce by budding, and are capable of converting sugar into alcohol and carbon dioxide. Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. Bread Alcohol Baker's yeast is the common name for the strains of yeast commonly used as a leavening agent in baking bread and bakery products, where it converts the fermentable sugars present in the dough into carbon dioxide and ethanol. mold mold Mold grows on bread when the bread comes in contact with dust that is carrying fungi, which are a tiny microorganisms. Spores remain airborne until they land on a piece of food, germinate and grow into a healthy fungus. mold Mold lives off the food its growing on by eating away at it. It commonly grows right through whatever it's eating. Fungi break down what they Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi are eating by digesting the juices they squirt out. They then grow microscopic roots into the food itself. hyphae A hypha (plural hyphae, from Greek ὑφή, huphḗ, “web”) is a long, branching filamentous structure of a fungus, oomycete, or actinobacterium. In most fungi, hyphae are the main mode of vegetative growth, and are collectively called a mycelium. Yeasts are unicellular fungi that do not grow as hyphae. Fungi play an important role in energy cycling within, and between, ecosystems. Fungi are found in terrestrial, marine and freshwater environments, and are part of a diverse community of “decomposers” that break down dead plants and animals. Mushroom cap is reproductive (fungi fruiting) structure. lichen A lichen is a composite organism that arises from algae or cyanobacteria living among filaments of a Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi fungus in a symbiotic relationship. The combined life form has properties that are very different from the properties of its component organisms. lichen Symbiosis in lichens is the mutually helpful symbiotic relationship of green algae and/or blue-green algae (cyanobacteria) living among filaments of a fungus.[1][2][3] The fungus benefits from the algae or cyanobacteria because they produce food by photosynthesis. The algae or cyanobacteria benefit by being protected from the environment by the filaments of the fungus, which also gather moisture and nutrients from the environment, and (usually) provide an anchor to it. The lichen combination of fungus and/or algae and/or cyanobacteria has a very different form (morphology),physiology, and biochemistry than the parts growing by themselves. The properties of the "whole" combination are very different from the sum of the properties of the parts living by themselves. lichen Symbiosis in lichens is the mutually helpful symbiotic relationship of green algae and/or Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi blue-green algae (cyanobacteria) living among filaments of a fungus.[1][2][3] The fungus benefits from the algae or cyanobacteria because they produce food by photosynthesis. lichen The algae or cyanobacteria benefit by being protected from the environment by the filaments of the fungus, which also gather moisture and nutrients from the environment, and (usually) provide an anchor to it. lichen The lichen combination of fungus and/or algae and/or cyanobacteria has a very different form (morphology),physiology, and biochemistry than the parts growing by themselves. The properties of the "whole" combination are very different from the sum of the properties of the parts living by themselves. lichen Schematic cross section of lichen, a symbiosis between green algae and a fungus. Thick layers of hyphae, called the cortex Green algae Loosely packed hyphae Lab 1 Notes – Diversity of Organisms: Bacteria, Protista and Fungi Anchoring hyphae called rhizines.