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18th International Symposium on Iron Nutrition and Interaction in Plants Madrid – May 30-June 3 2016 Interactions and functions of the transcription factor network for iron-regulated responses Daniela Lichtblau*, Birte Schwarz and Petra Bauer Institute of Botany, Heinrich-Heine University, Universitätsstraße 1, D-40225 Düsseldorf, Germany *[email protected] Iron (Fe) is an essential micronutrient for plants as well as animals. Despite the fact that a great amount of iron is present in the soil, most of it is insoluble and cannot simply be taken up by plants. Because of the strong requirement for Fe on one hand and the Fe-toxicity on the other hand, a very sensitive fine-tuned mechanism (up- and downregulation) of iron deficiency response genes is needed. To cope with changing environmental conditions the iron uptake system and homeostasis is controlled by a complex network of different transcription factors (TF) and other types of proteins [1]. In A. thaliana roots the FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) plays a major role for the regulation of iron uptake. As a heterodimer with one of the Ib subgroub bHLH´s transcription factors it regulates the expression of several iron deficiency response genes such as AHA2, FERRIC REDUCTION OXIDASE 2 (FRO2) and IRON-REGULATED TRANSPORTER 1 (IRT1) [2]. Besides FIT other transcription factors take part in the iron deficiency response. Some are directly regulated by FIT, others can be induced independently via the Fe status. FIT also interacts with transcription factors from other signaling pathways (EIN3, ZAT12) whereby the influence of different signaling pathways can lead to the activation/ inactivation of iron deficiency response genes [3,4]. It might be possible that the known transcription factors which form hetero- and homodimers among each other show a distinct hierachial cluster in order to regulate the expression of downstream iron response components. This also raises the question whether there are more yet unknown factors involved in initiating and maintaining the iron deficiency response. The aim of this work is to shed light on the hierarchy of transcription factors through investigation of protein-protein interactions. Keywords: iron homeostasis, transcriptional regulation, protein-protein interactions References: 1) Ivanov R., Brumbarova T., Bauer P. (2012) Molecular Plant 5(1):27-42 2) Yuan Y., Wu H., Wang N., Li J., Zhao W., Du J., Wang D., Ling H. (2008) Cell Research 18: 385397 3) Lingam S., Mohrbacher J., Brumbarova T., Potuschak Th., Fink-Staube C., Blondet E., Genschik P., Bauer P. (2011) The Plant Cell 23: 1815-1829 4) Le C., Brumbarova T., Stoof C., Weber E., Mohrbacher J., Frink-Staube C. Bauer P. (2016) Plant Physiol. 170(1): 540-557 1