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Biology 112 Lab Objectives Lab quiz # 1 Spelling counts on all lab quizzes. You are responsible for all objectives listed including assigned lab manual exercises and questions from lab reports. Exercise 4 – The Microscope Read lab exercises; then complete lab report # 3, pp. 39 parts A Be able to calculate total magnification using an objective lens Know following parts of the microscope and their functions. Complete lab report p. 40 Part D. Ocular lens Arm Rotating nosepiece Objective lenses Stage Stage clips Mechanical stage control Fine focus Coarse focus Light control Condenser Iris diaphragm lever Be able to determine why a slide is not viewable Be able to describe the proper way to carry a microscope Be able to describe proper way to store a microscope Chapter Two – Body Organization and Terminology Using models learn the following anatomical terms. Directional Terminology: Be able to answer lab report Part C p. 18 Superior/Inferior Anterior/Posterior Medial/Lateral Proximal/Distal Superficial/Deep Body cavities Fig. 2.1 p. 10 Thoracic Abdominal Pelvic Cranial Vertebral(spinal) Organs Be able to identify organs and place in proper cavity. Be able to answer lab report Part A p. 17 heart lungs brain spinal cord trachea esophagus stomach pancreas spleen liver small intestines large intestines ovary/testes urinary bladder Membranes Fig. 2.2 a and Fig. 2.2b P. 11. Where are these membranes found in the body? parietal pericardium visceral pericardium parietal pleura visceral pleura parietal peritoneum visceral peritoneum Planes Fig. 2.5 p. 13 Frontal(coronal) Sagittal Transverse Biology 112 Lab Objectives Abdominal quadrants (models) Fig. 2.6 B p. 14. RUQ LUQ RLQ LLQ Body regions Fig. 2.7 (a & b), pp. 15-16 Lab report D and E p. 19 Be able to label body regions on models Cephalic Cervical Vertebral Sternal Axillary Acromial Brachial Antebrachial Cubital Antecubital Carpal Palmar Umbilical Lumbar Sacral Gluteal Femoral Patellar Popliteal Plantar Exercise 5 – Cell Structure and Function Fig. 5.1 p. 42 Fig. 5.2 p. 43 Lab report #5 p. 46 part C Make your own cheek cell slide Use cell model to learn structures and functions of following cellular organelles Plasma (cell) membrane Cytoplasm Nucleus Nucleolus Nuclear envelope Chromatin Golgi apparatus Rough ER Smooth ER Mitochondria Lysosome Centrioles Ribosomes Exercise 6 – Movements through membranes If time permits: Perform lab procedure A. p.48 Perform lab procedure B. 48 Perform lab procedure C p. 49 Be able to define the following terms: Diffusion Osmosis Filtration What happens to a cell placed in each of these solutions? Hypertonic solutions Hypotonic solutions Isotonic solutions Biology 112 Lab Objectives Lab Quiz #2 Spelling counts on lab quizzes. You are responsible for all objectives listed including lab exercises and lab report questions. Exercise 7 – The Cell Cycle Fig. 7.2 p. 57 Lab report # 7 pp. 59-62 parts A, B, C, and D For test -- be able to identify the following stages of the cell cycle using whitefish blastula slides, photographs from p. 62 in lab manual, and from micrographs Interphase Prophase Metaphase Anaphase Telophase Exercise 8 – Epithelial Tissues Fig. 8.1 p. 64 Lab report # 8 pp. 65-66 parts A & B. * study the above sections for the test Using photographs and slides be able to recognize the following tissue types and cell types in italics. You do not have to identify where slides are located in the body. Stratified squamous - skin Simple squamous - lungs Simple columnar - stomach Simple cuboidal - kidney Pseudostratified columnar – trachea – goblet cell Transitional – bladder Chapter 9 -Connective Tissue Fig. 9.1 p. 68 Lab report 9 p. 69. (study for test) Adipose – adipose slide Hyaline cartilage – trachea chondrocyte Dense regular – tendon Loose (areolar) – areolar slide Blood – human blood slide – erythrocyte and leukocyte Bone – bone slide -osteocyte Chapter 10 - Muscle and nervous tissue Fig. 10.1 p. 74 (study for test) Skeletal muscle Smooth muscle Cardiac muscle – intercalated discs Nervous tissue - neuron Exercise 11- Integumentary System Fig. 11.1 p. 78 Fig. 11.2 p. 79 Using skin models and slides locate the following parts of the skin. Layers of the skin: Epidermis Dermis Hypodermis Skin structures: Hair follicle Hair shaft Arrector pili muscle Sebaceous gland Eccrine sweat gland Apocrine gland Biology 112 Lab Objectives Lab Quiz # 3 Spelling counts on lab quizzes. You are responsible for all objectives listed including lab exercises and lab report questions. Exercise 12: Bone structure Fig. 12.1 p.84 (study for test) Exercise 13-17 – Skeleton Fig. 13.1 p. 90 Fig. 13.2 p. 91 You are not responsible for any other figures in Chapter 13-17 other than those listed above. Identify the following bones and their features. Bone features are in parentheses and italics. Frontal Parietal Temporal ( mastoid process) (external auditory meatus) Occipital ( foramen magnum) Ethmoid ( crista galli) Sphenoid ( sella turcica) Vomer Nasal Zygomatic Maxilla ( palatine process) Palatine Mandible ( TMJ) Sternum( xiphoid process) (manubrium) Ribs ( true, false and floating) Cervical vertebrae Atlas Axis Thoracic vertebrae Lumbar vertebrae (spinous process) ( transverse process) (body of vertebrae) Sacrum Coccyx Scapula Clavicle Humerus – ( head of humerus) (trochlea) Ulna ( olecranon process) Radius ( styloid process) Carpals Metacarpals Proximal, Middle and Distal Phalanges Ilium Ischium Pubis (acetabulum) Femur ( head) (Medial and lateral condyles) patella Tibia ( medial malleolus) Fibula ( lateral malleolus) Tarsals - calcaneus Metatarsals Proximal, Middle and Distal Phalanges Exercise 18 Joints Locate the following joints on models. Coronal suture Sagittal suture Lambdoidal suture Symphysis pubis (Pubis symphysis) Sacroiliac joint Exercise 19 - 23 – Skeletal muscle Fig. 19.2 p. 140 You are not responsible for any diagrams on muscles. Be able to locate the following muscles on models. Frontalis Temporalis Orbicularis oculi Orbicularis oris Sternocleidomastoid External obliques Rectus abdominis Transverse abdominis Diaphragm Trapezius Latissimus dorsi Pectoralis major Deltoid Biceps brachii Triceps brachii Palmaris longus Extensor digitorum Gluteus maximus Gluteus medius Sartorius Quadriceps group Hamstring group Tibialis anterior Soleus Gastrocnemius Biology 112 Lab Objectives Lab Quiz # 4 Spelling counts on lab quizzes. You are responsible for all objectives listed including lab exercises and lab report questions. Exercise #24 – Nervous tissue and nerves Fig. 24.1 p. 174 Fig. 24.2 p. 175 Locate the following on the neuron model. Cell body Dendrite Nissl body(substance) Axonal hillock Axon Myelin sheath Node of Ranvier Parietal lobe Temporal lobe Occipital lobe Longitudinal fissure Transverse fissure Corpus callosum Thalamus Hypothalamus Pineal gland Midbrain Pons Medulla oblongata Cerebellum Exercise 29 - The Ear Fig. 29.1 p. 206 (know for test) Fig. 29.2 p. 207 Fig. 29.3 p. 208 Locate the following on ear models. Exercise 26 - The meninges and spinal cord Fig. 26.1 p. 188 Fig. 26.2 p. 188 Lab report # 26 part A p. 189 (study for test) Locate the following on the spinal cord model and on Fig. 26.2 p. 188. Central canal Gray commissure Anterior horn of gray matter Posterior horn of gray matter White matter Ventral root Dorsal root Dorsal root ganglion Spinal nerve Exercise 27 and Exercise 28– Brain and Cranial Nerves Fig. 27.1 p. 192 Fig. 27.2 p. 193 Follow directions for dissection on p. 197-201. Fig. 28.6 p. 199 is helpful for locating parts of the sheep brain. Locate the following on brain models, dissected sheep brain and on Fig. 27.1. Dura mater Pituitary gland Olfactory bulbs Optic chiasma Cerebrum Gyrus Sulcus Frontal lobe Pinna(auricle) External auditory meatus Tympanic membrane Malleus Incus Stapes Auditory tube Oval window Semicircular canals Vestibule Cochlea Vestibulocochlear nerve Exercise 30 – The eye Follow directions for eye dissection on pp. 217218. Fig. 30.8 p. 217 is helpful for learning the structures of dissected eye. Locate the following on eye models, dissected eye and from Fig. 30.3 p. 215 Extrinsic eye muscles Cornea Sclera Optic nerve Iris Pupil Lens Ciliary body Vitreous humor Choroid coat Retina Biology 112 Lab Objectives Lab Quiz #5 Spelling counts on lab quizzes. You are responsible for all objectives listed including lab exercises and lab report questions. Exercise 32- Endocrine System Fig. 32.1 p. 230 Fig. 32.8 p. 235 ( study for test) Fig. 32.9 p. 235 (study for test) Fig. 32.10 p. 236 (study for test) Be able to recognize the following endocrine glands using photographs and slides. Thyroid gland Adrenal gland Pancreas Recognize the following endocrine organs on models and be able to give the hormones each secretes. Pituitary gland Pineal gland Thyroid gland Parathyroid gland Thymus gland Pancreas Adrenal gland Testes Ovaries Exercise 33 – Blood cells Using photographs and slides recognize the following blood elements. Pictures on p. 242 are helpful. Erythrocytes Platelets Neutrophils Eosinophils Basophils Monocytes Lymphocytes Exercise 36 – The heart Fig. 36.1 p. 264 Fig. 36.2 p. 264 Fig. 36.3 p. 265 Fig. 36.6 p. 270 Diagrams above are helpful for learning the heart structures. Test questions on the heart will be on models and dissected sheep hearts. Follow directions for heart dissection, procedure B pp. 265-267. Locate the following on heart models and a dissected heart. Right atrium Left atrium Right ventricle Left ventricle Superior vena cava Inferior vena cava Pulmonary trunk Aorta Pulmonary veins Tricuspid valve Bicuspid valve Papillary muscle Chordae tendineae Aortic valve (model only) Pulmonary valve (model only) Exercise 38 – Blood vessels Fig. 38.1 p. 282 Fig. 38.3 p. 283 ( study for test) Be able to recognize an artery and a vein under the microscope. Locate the following vessels on models. You are not responsible for any diagrams in exercise 40, but they are helpful for learning the vessels of the body . Arteries: Coronary artery Brachiocephalic Common carotid Left subclavian Axillary Brachial Radial Ulnar Abdominal Common iliac Renal artery Femoral Anterior tibial Biology 112 Lab Objectives Veins: Cardiac veins Superior vena cava Inferior vena cava Brachiocephalic Jugular Subclavian Median cubital Renal Femoral Great saphenous Exercise 39 –Blood pressure If time permits this may be done as a demonstration. Perform procedure B p.288 Observe what happens to BP when sitting, standing up and after exercising. Blood pressure can be done as a demo if time is limited. Biology 112 Lab Objectives Lab Quiz # 6 Spelling counts on lab quizzes. You are responsible for all objectives listed including lab exercises and lab report questions. Exercise 42 – Digestive system Fig. 42.2 p. 312 Fig. 42.5 p. 314 Fig. 42.7 p. 315 Fig. 42.10 p. 317 (study for test) Fig. 42.11 p. 318 Fig. 42.12 p. 319 (study for test) Be able to recognize intestinal villi on a photograph or slide. Identify the following digestive system structures on models. Fig. 42.2 p. 312 Tongue Parotid gland Submandibular gland Sublingual gland Fig. 42.5 p.314 Nasopharynx Oropharynx Laryngopharynx Esophagus Fig. 42. 7 p. 315 Lower esophageal sphincter Cardiac region of stomach Fundus of stomach Body of stomach Pyloric region of stomach Pyloric sphincter Fig. 42.11 p. 318 Duodenum Jejunum Ileum Appendix Ileocecal sphincter Cecum Ascending colon Transverse colon Descending colon Sigmoid colon Rectum Fig. 42.10 p. 317 Pancreas Pancreatic duct Gallbladder Cystic duct Liver Hepatic duct Exercise 44- Respiratory system Fig. 44.1 p. 328 Fig. 44.2 p. 329 Fig. 44.6 p. 330 ( study for test) Be able to recognize alveoli from a photograph or slide. See Fig. 44.6 p. 331 Locate the following respiratory structures on models. Conchae ( nasal cavity) Hard palate Soft palate Uvula Pharynx ( 3 divisions) Epiglottis Larynx Trachea Bronchi ( primary and secondary) Bronchioles Alveoli Diaphragm The following exercise is optional. Instructors may include this exercise is time permits. Exercise 45 – respiratory volumes Fig. 45.4 (Spirogram) Perform procedure B p. 334 Handout can also be used for experiment. Turn in results to your instructor when finished. Exercise 46 – Kidney Fig. 46.1 p. 342 Fig. 46.2 p. 343 Fig. 46.4 p. 344 Procedure A pp. 341-343 (kidney dissection). Fig. 46.3 p. 343 is helpful learning the structures of a sheep kidney. Locate the following kidney structures on models and a dissected kidney. Biology 112 Lab Objectives Renal pelvis(region) Renal medulla(region Renal cortex(region) Renal pyramid Major calyx Minor calyx Ureter Locate the following structures on models only. Glomerulus Afferent arterioles Efferent arterioles Proximal convoluted tubules Nephron loop Distal convoluted tubule Collecting duct Renal artery Renal vein Bladder Urethra Interlobar artery/vein Interlobular artery/vein Arcuate artery/vein Exercise 48 – Male reproductive system Fig. 48.1 p. 356 Fig. 48.4 p. 357 (study for test) Locate the following female reproductive organ on models. Ovary Fimbriae Uterine tube Uterus Cervix Vagina Labia minorum Labia majorum Clitoris Bladder Urethra Genetics Oral Report: This oral report is to be presented after Lab Test Six. Report should last from 3- 5 minutes, Criteria: 1. Name of genetic disorder. 2. What is the pattern of inheritance? 3. Is it a dominant or a recessive trait? 4. What are symptoms of disorder? Be able to recognize seminiferous tubules from a photograph or slide. See Fig. 48.4 p. 357 Locate the following male reproductive organs on models. Testes Scrotum Epididymis Ductus deferens ( vas deferens) Seminal vesicle Prostate gland Bulbourethral gland Bladder Urethra Penis Glans penis OR Choose a disease or disorder named in the textbook. Check with me if you are unsure. Present a brief oral report on the disorder/disease on the night of the last lab quiz. The report needs to include the following: Name of disease/disorder System(s) and organ(s) involved How do individuals get it? (i.e.: parasite, genetic, bacterial, etc.) Symptoms Treatment or cure Exercise 49 – Female Reproductive System Fig. 49.1 p. 362 Fig. 49.4 p. 364 ( study for test) You also need to include a visual (picture, etc.) Be able to recognize an ovary from a photograph or slide. See fig. 49.4 p. 364 You will need to turn in some type of written documentation used for the report (notecards, informal paper, etc) Biology 112 Lab Objectives You may do a powerpoint presentation. In that case, no other written documentation is needed. Upon successful completion of the oral report, 7 points will be added to the last lab quiz.