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Science 8 Biology Unit Chapter 1 – Cells KEY Pages 4 – 39 Section 1.1 – Characteristics of Living Things 1. List the 6 characteristics of living things. Refer to Table 1 on page 5. a) b) c) d) e) f) Are composed of cells and all cells are similar Respond to the environment. Have a life span. Reproduce, grow and repair themselves. Require energy. Produce waste. 2. Define organism: an individual living thing 3. What are the two main ideas of the cell theory? All living things are composed of one or more cells. All new cells arise only from cells that already exist. Section 1.2 – The Microscope (refer to pages 396 – 398) 1. Describe the method you would use to prepare a dry mount. a) Place the object that you wish to observe in the centre a microscope slide. b) Hold a cover slip between your thumb and forefinger. Place the edge of the cover slip to one side of the object and gently lower the cover slip onto the slid so that it covers the object. 2. What is the difference between a dry mount and a wet mount? How do you put the cover slip over an object when doing a wet mount? The difference between a dry mount and wet mount is that a dry mount does not use water. You will place 2 drops of water on the object then holding the cover slip with your thumb and forefinger, touch the edge of the surface of the slide at a 45 degree angle and gently lower the cover slip, allowing air to escape. 3. What power setting should your microscope be on when placing a slide onto the stage of a microscope? Low-power setting 1 4. You first bring an image into focus using the coarse-adjustment knob. Why do you not want the lens to touch the cover slip? You may move the cover slip and therefore your object; you may smudge the lens; you make break the lens; you make break the microscope lens. 5. Why do you place the object on the microscope slide facing the “wrong” way and upside down? What happens when you move the object away from your body while looking through the eyepiece? You place an object facing the “wrong” direction and upside down so that it appears the “right” way and upright when you look through the eyepiece. When you move the object away from your body, it appears to be move towards you. 6. Define field of view: The circle of light you see when you look through the eyepiece of a microscope. You will see your object within the field of view. 7. How do you get an object to appear larger when using a microscope? What adjustment knob would you use to bring the letter into focus? Rotate the nosepiece to the medium-power or high-power objective lens. Use the fine adjustment knob. 8. What steps do you use when storing a microscope? a. Rotate the nosepiece to the low-power objective lens. b. Remove the slide and the cover slip. c. Clean the slide and cover slip, then return them to their appropriate location. d. Return the microscope to the storage area. 9. What is a stereo microscope or dissecting microscope? When would you use this microscope? A microscope used to observe small 3-D objects. You use this microscope when you cannot look at a sample on a slide. Ex. Live specimens. 2 Section 1.3 – Plant and Animal Cells 1. State the difference between eukaryotic and prokaryotic cells. Eukaryotic cells are cells that have a nuclear membrane and prokaryotic cells are one-celled organisms that where the nucleus is not surrounded by a membrane. 2. Animal Cell Structures – give the description and function for the following cell structures as seen through a compound light microscope: Cell Structure Nucleus Chromosomes Cell Membrane Cytoplasm Description Surrounded by a membrane Found inside nucleus Surrounds the cell Double layer of fat molecules Watery fluid that contains everything inside the cell. Vacuole Filled with fluid Flagellum Whip-like tail Not found on all cells Tiny hairs Not found on all cells Cilia 3. Draw Figure 2, page 11 in the space below. 3 Function Control centre Directs all of the cell’s activities Contains genetic information (DNA); genetic info duplicated and passed on to other identical cells Holds contents of cell in. Controls movement of nutrients in and wastes out. Chemical activities occurs here Allows materials to be transported quickly in the cell Stores wastes Stores water and nutrients Stores waste and excess water Helps cells to move Helps move a cell Helps move fluid surrounding the cell 4. Plant Cell Structures - give the description and function for the following cell structures as seen through a compound light microscope: Cell Structures Vacuole Cell Wall Description Same as animal cell, except that it is much larger Thick outer wall Chloroplasts Contain chlorophyll Function Stores water and nutrients Stores wastes Provides protection, extra support, and strength. Food factories Chlorophyll allows plants to make their own food using the sun, CO2 and water. 5. List the similarities and differences between plant and animal cell structures. Similarities – both have a nucleus, chromosomes, cell membrane, cytoplasm and vacuole. Differences – only some animal cells have flagellum and cilia, while plant cells have much larger vacuoles, a cell wall, and chloroplasts. 6. Where in the cell do you find genetic information? Chromosomes contain genetic information and are found in the nucleus. 7. Predict what might happen to a cell if the cell membrane was replaced by a plastic covering that prevented molecules from entering or leaving the cell. The cell will die because no nutrients can come in and there would be a build up of waste products inside the cell (becomes toxic to the cell). 8. Cilia also function to remove dirt and debris. Where in the human body might you find cells with cilia? Explain your answer. In your throat and lungs. 4 Section 1.5 – Technological Advances of the Microscope 1. Complete the following chart on the different types of microscopes. Type of Microscope Single-Lens Microscope Compound Light Microscope Transmission Electron Microscope Scanning Electron Microscope Capabilities Was able to magnify objects 10 X. Able to see tiny organisms 2nd lens added; 2000X magnification Some organelles can be seen 2, 000, 000 X magnification Uses beam of electrons Can make a 3D image of the specimen from reflecting electrons Limitations Can only magnify 10 X so can’t see the details of an organism Lenses are made with glass; as the lens gets thicker, the image becomes blurred. Specimens with many layers cannot be examined Can only view dead cells (because you must make thin sections of the cell) Only the outside of the specimen can be seen Cannot magnify as much as TEM 2. Give one advantage of a scanning electron microscope over a transmission electron microscope. The scanning electron produces a 3D image from the reflection of electrons off a specimen; therefore, the thickness of the specimen does not matter (as it did in the transmission e- microscope). 3. Describe the differences in appearance of algae cells when viewed with each of the different types of microscopes. As you go from the single-lens microscope to the transmission electron microscope, more detail of organelles can be seen. The scanning electron microscope can give a 3D image of the algae, instead of a 2D, flat image. 4. Which microscope would you recommend for viewing each of the following? Give reasons for your choice. a) the detailed structure of a cell’s nucleus: If you wanted to keep the specimen alive, I would use a compound light microscope, but for the most detail, then the transmission electron microscope (it’s magnification is 2,000,000X). b) the outside of a single cell: I would use the scanning electron microscope to get a 3D image of a single cell and it wouldn’t matter what thickness the specimen was. 5 Section 1.6 – Parts of a Cell Seen with an Electron Microscope 1. Define organelles: Tiny structures within a cell. The parts of a cell that each of specific functions. 2. What contains the organelles of the cell? Cytoplasm 3. Which organelle is generally the largest of the cytoplasmic organelles? Mitochondria 4. Complete the following chart on organelles found in both plant and animal cells when using a transmission electron microscope. Cell Structures Mitochondria Ribosomes Very small (fuzzy dots when use TEM Endoplasmic Reticulum Series of folded membranes “Rough” ER – where ribosomes attach “Smooth” – no ribosomes attached Looks like stack of flattened balloons Contain special proteins that breakdown larger molecules into many smaller molecules (which can be reused) Formed by the Golgi apparatus Golgi Apparatus Lysosomes Description Circular or rod-shaped Function Energy production “Power plants” Produce proteins Proteins needed for cell growth, repair, and reproduction Carry materials through cytoplasm SER – fats (lipids) produced here Stores proteins; puts into packages called vesicles to carry to outside of cell Destroy harmful substances and invading bacteria Break large molecules into small molecules which can then be reused as building blocks 5. What is the singular word for mitochondria? mitochondrion 6. Write the word equation for cellular respiration in the box below. Where is the energy produced using cellular respiration used? In almost every other function of the cell Sugar (carbohydrates) + oxygen Carbon dioxide + water + energy 6 7. What is the difference between “rough” endoplasmic reticulum and “smooth” endoplasmic reticulum? RER has ribosomes attached to it, while SER does not. 8. Cells lining the stomach release enzymes that aid digestion. Digestive enzymes are protein molecules. Explain why many Golgi apparatuses are found in stomach cells. The Golgi apparatus stores proteins; therefore, when digestive enzymes are being made, they need protein. Digestion occurs in the stomach so it makes sense for proteins to be stored there. Section 1.7 – Cells in Their Environment 1. What is the difference between the words permeable and impermeable? Permeable means to allow all materials in and out and impermeable means that no materials enter or leave. 2. Why are cells membranes called selectively permeable? What is another name for selectively permeable? Cell membranes allow certain materials to enter or leave, but not others. Semi-permeable. 3. Define diffusion: the movement of molecules from an area of high concentration to an area of lower concentration. 4. Diffusion continues until the concentration of the substance is the same inside and outside the cell. 5. Draw the movement of oxygen and carbon dioxide into and out of a cell using the process of diffusion. Use the letter O to represent oxygen and CO2 to represent carbon dioxide. Oxygen Carbon Dioxide 7 6. Do you think cells could survive without diffusion? Explain why or why not. No, cells would not survive because there would be no movement of nutrients into the cell and no movement of wastes out of the cells. 7. Speculate on what would happen if cell membranes were permeable instead of selectively permeable. All sorts of molecules could enter the cell, including molecules that might be harmful to the cells. 8. Describe two situations in your everyday experience where substances are spread around by diffusion. Smelling perfume; smelling dinner; smelling an air freshner Section 1.8 – Osmosis 1. Define osmosis: The diffusion of water. Water will diffuse from an area of high concentration of water to an area of lower concentration of water. 2. What does “concentration” mean? The amount of particles that are the same (ie. Perfume molecules/particles in a bottle will have a high concentration since there are more of them in the bottle then in the air) The amount of a specified substance in a unit amount of another substance. 3. Define solute and name two common solutes found in cells: Solute is a substance that is dissolved in another substance. Two common solutes are salts and sugars 4. Define solvent: The substance that dissolves the solute. Common solvent is water. 5. Refer to Figure 2 on page 25 to answer the following questions: a) In Figure 2(a), the small blue circles indicate which molecule, water or protein? water b) In Figure 2(a), the concentration of water is greater on which side, X or Y? X c) In Figure 2(a), what way will water diffuse and why? Water will diffuse from X to Y (left to right) because there is a higher concentration of water molecules in X than in Y. 8 6. Refer to Figure 3 on page 26 to answer the following questions: a) In Figure 3(a), the movement of water into and out of the cell is balanced. The size and shape of the cell will remain the same. b) In Figure 3(b), the concentration of solutes outside the cell is less than inside the cell. Water will move into the cell. The size of the cell will increase. c) In Figure 3(c), the concentration of solutes inside the cell is less than outside the cell. Water will move out of the cell. The size of the cell will decrease. 7. What determines the direction of water movement into or out of cells? Water moves from an area of high concentration to an area of low concentration. 8. Explain why animal cells are more likely than plant cells to burst when placed in distilled water. Plant cells have the added protection of a cell wall, while animal cells do not; therefore, animal cells may burst if too much water enters. 9. What happens if too much water leaves a cell? The cell will die (water is needed for chemical reactions to occur). 10. Describe turgor pressure: pressure created inside a plant cell when water enters by osmosis. Water fills the vacuoles and cytoplasm, causing them to swell up and push against the cell wall. 11. How are osmosis and diffusion different? How are they the same? Osmosis and diffusion are different because osmosis is the movement of water while diffusion is the movement of molecules. They are the same because the movement is from high concentration to low concentration. 9