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CELLULAR RESPIRATION Living organisms require energy What is the usable form of energy for cells? ATP Used for mechanical, chemical and transport work. What is the ultimate source of energy? SUN * Eukaryotic organisms (plants, protists, fungi and animals) use the O2 and the stored potential energy in the organic molecule (“Glucose”) during Cellular Respiration to produce usable potential energy ATP. CO2, H2O and heat are waste products. What organelle do eukaryotic cells use for Cellular Respiration? Mitochondria Describe the relationship between Photosynthesis and Cellular Respiration: Cellular Respiration (Simplified) * 40% of energy in glucose converted to ATP, 60% into heat Identifying Oxidation and Reduction in Reactions The oxidation number of an element indicates the number of electrons lost, gained, or shared as a result of chemical bonding. Oxidation Oxidation is an increase in the oxidation number of the element which is associated with loss of electrons. From this we get the abbreviation OIL: Oxidation is Loss (of electrons). Reduction Reduction is the opposite of oxidation so it is no surprise that reduction is defined as a decrease in the oxidation number of the element which is associated with gain of electrons. This gives us a second abbreviation RIG: Reduction is Gain (of electrons). OILRIG OILRIG is a convenient and easy way to remember the rules for electron loss and gain in oxidation reduction reactions. OIL: Oxidation is Loss (of electrons). RIG: Reduction is Gain (of electrons). LEO says GER GERRRR LEO – Losing Electrons is Oxidation GER – Gaining Electrons is Reduction SUBSTANCE X - LOSES e : OXIDIZED X loses electrons X is oxidized X increases oxidation number SUBSTANCE Y - GAIN e : REDUCED Y gains electrons Y is reduced Y decreases oxidation number The oxidation number of an element indicates the number of electrons lost, gained, or shared as a result of chemical bonding. Cellular Respiration… keep your eyes on the electrons (hydrogen). Energy is contained in the arrangement of electrons in the chemical bonds that hold organic molecules together. Electrons lose energy as they “fall”/are transferred to oxygen. The energy released can be stored in ATP. NAD+ = Nicotinamide Adenine Dinucleotide (coenzyme) Cellular Respiration Overview Stage 1: Glycolysis Occurs in the cytoplasm Breaks glucose into 2 pyruvate Removes electrons (Hydrogen atoms) Generates ATP Stage 2: Citric Acid Cycle (Krebs Cycle) Occurs in the mitochondrial matrix Carbons released as CO2 Removes electrons Generates ATP Stage 3: Oxidative Phosphorylation (ETC and Chemiosmosis) Occurs at the inner mitochondrial membrane (Cristae) Electrons and protons used to generate ATP Water produced Glycolysis – “Splitting of Glucose” Located in the cytoplasm of the cell In a series of chemical reactions, a 6-carbon organic molecule, Glucose, is split into 2, 3-carbon molecules called pyruvate (pyruvic acid). o First phase – energy invested – used to prepare glucose and intermediate molecules for breaking. o Second phase – energy harvested – NADH and ATP molecules are produced. Important Numbers 2 ATP are invested 2 NAD+ are reduced to 2NADH + 2H+ (2NADH2) 4 ATP are produced ATP Net Gain? 2ATP Substrate-level Phosphorylation – an enzyme transfers a phosphate group from a substrate molecule directly to ADP to form ATP. https://www.youtube.com/watch?v=3GTjQTqUuOw https://www.youtube.com/watch?v=iChb5hEDzqc Glycolysis – might be an ancient metabolic (energyharvesting process) pathway. Found in all types of cells (bacteria humans) Does not require oxygen Does not require membrane bound organelles