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Technical Report of secondment First name: Family name: Name of work organization Position Date of trip Name of country Name of University Project Name Aim of project Aim of visit Results Vitaliy Bolotin National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkov, Ukraine Head of the Department for Molecular Diagnostics 01.07.2015 – 31.07.2015 Germany The Department of Environmental and Animal Hygiene, at the Institut für Nutztierwissenschaften, of the University of Hohenheim Anthrax Environmental Decontamination Network To achieve effective and ecologically friendly means of reducing spore numbers in the environment and transfer knowledge between the participant Practical and theoretical training concerning full characterized anthrax spore contaminated test sites. I familiarized with a number of molecular-genetics methods such as high-resolution molecular assays including multiple-locus variablenumber tandem repeat analysis (MLVA), canonical single-nulceotide polymorphism (cSNP) analysis, and single-nucleotide repeat (SNR) analysis will be used in the epidemiological analysis environmental isolates. It was characterized 10 isolates according to modern system of copy-codes and their typing was provided. As a part of this work I trained to cultivate different spore-produced bacteria. I isolated spores of B. anthraces from soil samples and carried out different test to define biological properties (phage, antibiotics, staining and microscopy). Technical Report of secondment First name: Family name: Name of work organization Position Date of trip Name of country Name of University Vasyl Kosheliev National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkov, Ukraine PhD-student at the Department of Avian Viral Diseases 01.07.2015 – 31.07.2015 Germany The Department of Environmental and Animal Hygiene, at the Institut für Nutztierwissenschaften, of the University of Hohenheim Project Name Anthrax Environmental Decontamination Network Aim of project To achieve effective and ecologically friendly means of reducing spore numbers in the environment and transfer knowledge between the participant Training concerning full characterized anthrax spore contaminated test sites Aim of visit Results I had theoretical and practical training concerning high-resolution molecular assays including multiple-locus variable-number tandem repeat analysis (MLVA), canonical single-nulceotide polymorphism (cSNP) analysis, and single-nucleotide repeat (SNR) analysis will be used in the epidemiological analysis environmental isolates. 10 isolates were characterized according to copy-codes and their typing was provided. I trained as well cultivation of different spore-produced bacteria. Within this work I isolated spores of B. anthraces from soil samples and carried out different test to define biological properties (phage, antibiotics, staining and microscopy).
