Download Anti-Mouse Corticotropin-Releasing Hormone (CRH)

Anti-Mouse Corticotropin-Releasing Hormone (CRH)
Polyclonal Antibody
CL8859AP
Lot: 165921
DESCRIPTION:
Corticotropin-releasing hormone (CRH) or corticotropin-releasing factor (CRF) is a peptide hormone secreted by the
paraventricular nucleus (PVN) of the hypothalamus in response to stress. It’s a key player in the hypothalamic
pituitary adrenal axis mainly functioning to stimulate pituitary synthesis of adrenocorticotropic hormone (ACTH). In
addition to stress response, neuronal CRH is important in a number of other diverse roles including, memory,
depression, kidney function, metabolism and feeding. Elevated CRH expression has been shown to be associated
with Alzheimer’s disease as well as major depression1.
In addition to production in the hypothalamus, CRH is also synthesized in peripheral tissue such as T lymphocytes
and is highly expressed in the placenta. In the placenta, CRH is a marker that determines the length of gestation and
the timing of parturition and delivery2.
PRESENTATION:
100 µg purified IgG buffered in PBS and 0.02% NaN3. (Purified from serum via Affinity Chromatography). For
maximum recovery of contents, spin down tube before use.
STORAGE/STABILITY:
Store at + 4°C. For long term storage, aliquot and freeze unused portion at -20°C in volumes appropriate for single
usage. Avoid freeze/thaw cycles.
SPECIFICATION:
Immunogen: 13 amino acid synthetic peptide located at the C-terminus of mouse CRH.
Specificity: This antibody is specific for mouse, rat and human CRH.
IgG Class: Rabbit IgG
Application: This antibody is suitable for use in ELISA and Western Blot (0.03 µg/mL). This antibody has not been
tested in other applications.
Continued Overleaf…..
TEST RESULTS:
Western Blot:
CL8859AP (0.03µg/ml) staining of human brain cerebellum (A), mouse brain (B) and rat brain (C) lysates (35µg
protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
N.B.
Appropriate control samples should always be included in any labeling studies.
* For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate
for individual use.
REFERENCES:
1.
2.
Raadsheer FC, et al. (1995) Corticotropin-releasing hormone mRNA levels in the paraventricular nucleus
of patients with Alzheimer's disease and depression. Am J Psychiatry. 152(9):1372-6.
Jones SA, Brooks AN, Challis JR. (1989) Steroids modulate corticotropin-releasing hormone production in
human fetal membranes and placenta. J Clin Endocrinol Metab. 68(4):825-30.
For Research Use Only
JK 11/09/16