Download Muscle Histology

Lab 7
Microscopic Anatomy and Organization of Skeletal Muscle - Chapter 10
in Text
Histology- Chapter 4 and Chapter 10 in text
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Study the structure of skeletal muscle and the neuromuscular junction. Identify
axon, axon terminal, and skeletal muscle cell
Study the structure of cardiac muscle. Identify branched cells, nuclei, striations and
intercalated discs.
Study the structure of smooth muscle . Identify nuclei and muscle cells.
Gross Anatomy of the Muscular System (Chapter 11, Table 11 in text.)
Begin Muscle Anatomy – Do this part to prepare for Lab Exam 2 at the end of the
semester. On the figures of anatomical models, begin to locate all of the muscles on the
attached list. It may help to demonstrate the muscle action as you locate each muscle. You
are responsible for the identification and action of the muscles on the lab exam. Please
use the action from your text. Choose one major action that you will remember.
In Lab Activity
1. Each lab bench has been assigned three muscles. Create a model of each muscle using
clay and attach it correctly to the skeleton. Include the tendons as part of the muscle so
that you can show the correct origin and insertion. Model all three muscles on the same
skeleton. Each member of the group will present one muscle from their list during the last
30 minutes of lab. In your demonstration each member of the group will:
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Introduce yourself (10%)
Identify a muscle on the skeleton. (10%)
Identify the origin and insertion of the muscle. (20%)
Demonstrate the action of the muscle (what happens when it contracts). (10%)
The model, demonstration and oral presentation is 50% of your lab 7 grade. You
are all responsible for the quality and accuracy of the demonstration. Each person
in the group must speak during the presentation.
Due at the end of lab or at the next class.
Carefully draw and label the three muscles your group demonstrated in class on the
attached figures. 5 points each.
Muscle Assignments for Lab Tables
Table
Number
Muscle 1
Muscle 2
Muscle 3
1
deltoid
masseter
erector spinae
2
latissimus dorsi
zygomaticus
major
rectus femoris
3
tibialis anterior
temporalis
biceps femoris
4
vastus lateralis
biceps brachii
gastrocnemius
5
trapezius
triceps brachii
rectus abdominis
6
pectoralis major
epicranius occipital and
frontal bellies
gluteus maximus
7
platysma
brachioradialis
gracilis
8
sternocleidomastoid semitendinosus
sartorius
Name____________________________
Lab Section ________________
Lab 7 – Muscle Anatomy – turn in at the end of lab or at the next class.
1. Draw and label the three muscles demonstrated by your group where appropriate on these
figures. Label the muscle by name, label the origin and name of origin and label the insertion
and the name of insertion. 10 points each
Name
2. Obtain slide of skeletal muscle with a neuromuscular junction (motor end plate).
Draw a picture of the slide below and label: muscle cell, nucleus, striations, axon, and
axon terminal (at neuromuscular junction). Use a colored pencil, a ruler for the
leader lines and print the labels. Colored pencils are available in the lab. Use the
40X lens for the drawings. Indicate the total magnification. 10 points
Neuromuscular Junction, Total Magnification _______ X
3. Obtain a slide of smooth muscle. Find a section where the muscle is in longitudinal
section. Draw a picture of the slide below and label: muscle cell, nucleus,
myofilaments. Use a colored pencil, a ruler for the leader lines and print the
labels. Colored pencils are available in the lab. Use the 40X lens for the drawings.
Indicate the total magnification. 5 points
Smooth Muscle, Total Magnification _______ X
4. Obtain slide of cardiac muscle. Draw a picture of the slide below and label: branched
muscle cell, nucleus, striations, intercalated disc. Use a colored pencil, a ruler for the
leader lines and print the labels. Colored pencils are available in the lab. Use the
40X lens for the drawings. Indicate the total magnification. 5 points
Cardiac Muscle, Total Magnification _______ X
For the Lab Exam
Muscle Histology
Identify on figures:
A band
Blood vessels
Endomysium
Epimysium
Fascicle
H zone
I band
M line
Mitochondrion
Muscle cell
Myofibril
Nucleus
Perimysium
Sarcoplasmic reticulum
T tubule
Terminal cisternae
Triad
Z disc
Identify the following slides
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smooth muscle (cell, nucleus)
cardiac muscle (cell, nucleus, striations, intercalated discs)
skeletal muscle (cell, nucleus, striations)
neuromuscular junction (striations, axons, axon terminals) .
You are responsible for the location and action of these muscles on figures.
Ventral Muscles of the Trunk and Neck (Generally)
external oblique
pectoralis minor
internal oblique
platysma
pectoralis major
rectus abdominis
Dorsal Muscles of the Trunk and Neck, Generally
deltoid
latissimus dorsi
erector spinae
subscapularis
infraspinatus
supraspinatus
serratus anterior
sternocleidomastoid
transversus abdominis
teres major
teres minor
trapezius
Muscles of the Head and Neck
epicranius (occipital
belly)
epicranius (frontal belly)
masseter
orbicularis oculi
orbicularis oris
temporalis
zygomaticus major
Muscles of the Upper Limb (Generally)
biceps brachii
brachialis
brachioradialis
extensor carpi radialis
longus
extensor carpi ulnaris
extensor digitorum
(forearm)
flexor carpi radialis
flexor carpi ulnaris
palmaris longus
triceps brachii – lateral,
medial, and long
heads
Muscles of the Lower Limb (Generally)
adductor longus
adductor magnus
biceps femoris
extensor digitorum
longus
gastrocnemius
gluteus maximus
gluteus medius
gracilis
pectineus
rectus femoris
sartorius
semimembranosus
semitendinosus
soleus
tensor fasciae latae
tibialis anterior
vastus lateralis
vastus medialis
Muscle Worksheet. Please use the text or lab manual and brief but correct
descriptions of the origin, insertion and action of each muscle. You are only
responsible for identification and action on the lab exam.