Download Animal cell lines

CELL LINE
When primary culture is first subcultured or
passaged , it became cell line.
ANIMAL CELL CULTURE:
When primary culture of animal cells is first
subcultured or passaged , it became animal cell
line.
THREE TYPES OF CELL LINE
CELL LINE
FINITE
CELL LINE
Normal cell usually
divide only a limited
number of times
before loosing there
ability to proliferate
which is a
genetically
determined event
known senescence.
CONTINUOUS
CELL LINE
When a finite cell
line undergoes
transformation
and acquires the
ability to divide
indefinitely .
TRANSFORMED
CELL LINE
Conversion of a
normal healthy cell
into tumorous and
cancerous cell.
FEATURES
FEATURES
Ploidy
Anchourage
Dependence
Density limitation
Of growth
Serum requirement
Cloning efficiency
Growth rate
Yield
FINITE
diploid
yes
yes
high
low
slow (24-96 ) hour
low
CONTINUOUS
heteroploidy
no
no
low
high
high (12-24)hours
high
FINITE CELL LINE
CELL LINE
IMR-90
MRC-5
293
 B16
 LLC-PKI
ORIGIN
Lung
Lung
Kidney
kidney
SPECIES
human
human
human
rat
pig
CHARACTERISTICS
Susceptible to human viral
infection.
Susceptible to human viral
infection.
readily transfected.
melanin
Na+- dependent glucose uptake.
CONTINUOUS CELL LINES
CELL LINE
ORIGIN
 Caco-2
Colon
 Friend
spleen
 P388D1
 GH1,GH2
 S180
SPECIES
Human
Mouse
Mouse
Rat
Mouse
CHARACTERISTICS
transport ions and amino acids
hemoglobin
grow in suspension
growth hormone
Cancer chemotherapy screening
TRANSFORMED CELL LINES
CELL LINE
3T3
Vero
HeLa
ORIGIN
kidney
epithelial cell
SPECIES
Mouse
monkey
human