Download GelElectrophoresisAmoebaSistersVideoHandout-1

GEL ELECTROPHORESIS
Name: ___________________________
VIDEO HANDOUT
This handout accompanies the Gel Electrophoresis video by the Amoeba Sisters on YouTube.
Complete the Before the Show section and try to guess the answers to the Showtime
questions before starting the video.
Full URL: https://www.youtube.com/watch?v=ZDZUAleWX78&t=336s
Tiny URL: https://tinyurl.com/mbnw6z3c
B E F O R E T H E S H O W (answer before you start video)
Think of the term biotechnology. What do you think biotechnology is? Brainstorm all possibilities.
S H O W T I M E Q U E S T I O N S (answer while viewing the video)
1. Which of the following is not a type of
biotechnology?
a. polymerase chain reaction
b. cloning
c. virtual reality technology
d. genetic engineering
2. How does gel electrophoresis separate
molecules?
a. by size
b. by shape
c. by color
d. by sequence
3. What charge does a DNA molecule have?
a. positive
b. negative
c. neutral
4. What is the type of gel that is typically used in a
gel electrophoresis machine?
a. gelatin
b. monosaccharide
c. agarose
5. In which location within the gel electrophoresis
machine are DNA samples placed into?
a. wells
b. electrodes
c. center of the gel
6. What type of enzymes is used to cut DNA into tiny
pieces?
a. ligase
b. restriction enzymes
c. recombinant enzymes
7. Why are the samples of DNA in the mother and
baby guppy differently sized?
a. differences in DNA base sequences
b. differences in enzymes
c. differences in age of DNA
8. Which piece of DNA will take the longest to move
through the gel?
a. a small fragment of DNA
b. a medium-sized fragment of DNA
c. a large fragment of DNA
9. What can you compare to see how closely related
two organisms are using a gel electrophoresis
machine?
a. DNA bands
b. agarose bands
c. the size of DNA fragments
10. What is a DNA ladder used for?
a. moving samples through the gel
b. identifying DNA base pair length
c. finding known DNA fragment sizes
11. Which of the following is NOT a reason why
scientists use gel electrophoresis?
a. determines relatedness in different species
b. helps to better classify organisms
c. DNA fingerprinting
d. make copies of DNA fragments
12. Why is DNA fingerprinting helpful?
a. it can isolate genes of interest
b. it can help to identify someone’s DNA
c. it can help to locate DNA in a crime scene
© SCIENCE IS REAL
A F T E R T H E S H O W (answer after viewing the video)
Use the word bank to label the diagram of a gel electrophoresis machine and complete the crossword puzzle.
Word Bank
• DNA Band
• Long DNA Fragment
• Negatively Charged Side
• Positively Charged Side
• Short DNA Fragment
• Well
1
2
Across
2.
tool useful for identifying DNA base pair length
5.
separates molecules by size
6.
a term that describes the merge of biology and
technology
8.
a biotechnology used to identify someone’s DNA
10. location where samples are place in a gel
electrophoresis machine
11. lines produced during the process of gel
electrophoresis
3
4
5
6
Down
1.
polysaccharides are this type of
macromolecule
3.
type of gel used in gel
electrophoresis
4.
cuts DNA up into fragments
7.
building blocks of DNA
9.
the charge of DNA
7
8
9
10
11
Extension
Choose one of the following biotechnologies. Research the biotechnology and write a short summary of
what it is and what it is used for.
Choices: Polymerase chain reaction (PCR), DNA cloning, CRISPR, DNA Sequencing
© SCIENCE IS REAL