Download Lab on chip for rapid diagnosis of infectious diseases

FRAUNHOFER INSTITUTE FOR CELL THERAPY AND IMMUNOLOGY IZI
LAB-ON-CHIP FOR RAPID
DIAGNOSIS OF INFECTIOUS
DISEASES
Currently a reliable diagnosis of complex
Unique feature
and life-threatening infectious diseases
like sepsis is only possible using elaborate
The MinoLab lab-on-chip device is based
and time-consuming methods involving
on magnetic microparticles, which combine
different functionalities:
Ŷ Allow effective capturing of bacteria- or
specialists. Together with different
partners from Industry and with support
Fraunhofer Institute for
of the German Ministry for Education
Cell Therapy and Immunology IZI
and Research (BMBF), the Fraunhofer IZI
develops an innovative system for rapid,
Perlickstraße 1
easy-to-conduct and inexpensive on-site
04103 Leipzig
infection diagnostics called MinoLab.
Germany
fungal cells out of patients samples
Ŷ Enable electromagnetic transport of
attached target cells through the labon-chip device
Ŷ transport
Ŷ Microparticles develop a detectable
signal on the surface of a magneto-
Contact
resistive DNA chip
Dr. Dirk Kuhlmeier
Department of Diagnostics
Method
Nanotechnology Unit
Phone +49 341 35536-9312
The core of this novel device is the nano-
[email protected]
meter- to micronmeter-sized magnetic
particle that can be functionalized
www.izi.fraunhofer.de
Technology principle
separation
magneto
sensor
Cell isolation
DNA isolation
particles and all necessary reagents are
amplification
Potential applications
Nanotechnology Unit
card format. In case of an on-site examina-
Sepsis, blood poisoning, is one important
In the following areas partners from
tion, a sample, e. g. blood, saliva or urine,
example for a potential application. About
is taken from the patient and applied onto
half of the approximately 154,000 annual
the card. Upon lysis of the target cells, the
cases of sepsis in Germany must be treated
from experienced and reliable people:
Ŷ Development of integrated
magnetic particles bind to the respective
in intensive care and still more than 40
target molecules contained in the sample
percent of those cases treated result in the
and are transported in a fully automated
lab-on-chip devices
Ŷ Assay development
Ŷ Rare cell diagnostic
manner through the individual reaction
to the fact that sepsis can be caused by
Ŷ Nucleic acid isolation and
cavities via magnetic forces. At the end of
dozens of different pathogens. Moreover,
the process chain, detection is conducted
individual classes of these pathogens have
Ŷ Functionalization of surfaces in
by means of highly sensitive magnetic
already developed a resistance to certain
sensor technology – the signals are
therapeutic agents. The time between
digitalized and a fully electric read-out
blood sample collection and obtaining a
the nanometer scale
Ŷ Sensor development
Ŷ Test strip development
is performed.
diagnostic result still amounts to at least
Ŷ DNA origami techniques for drug
stored on a single-use cartridge in check
delivery or systematic surface
eight hours. By integration of all process
steps from sample preparation to detection
on the innovative diagnostic platform,
we are aiming for a time-to-result of less
than two hours. In addition to medical
applications such as proteomic, genomic
and microbiological tests, the future areas
of application also comprise environmental
analyses or civil protection measures.