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Seroprevalence of Toxoplasma gondii in pregnant women in some areas of Basrah Province, Iraq Khalid J. K. Al-Zihiry Department of Microbiology , College of Medicine, University of Thi-Qar. Mahdi M. Thueni Department of Pathology , College of Medicine, University of Thi-Qar. Kassim H. Khalaf M.Sc. Biochemistry, Al-Qurna General Hospital, Basrah Abstract The current study was carried out to detection the seroprevalence of Toxoplasma gondii in pregnant women in some areas of Basrah Province (Al-Qurna and Talha) by examination of 63 blood serum in Al-Qurna hospital for the period from January to June 2007 using indirect haemagglutination test. From collected samples 50 (79.3 %) was positive for T. gondii antibodies in different dilutions. No significant differences in seroprevalence between age groups of examined pregnant sera. Also it showed the strongly correlation between the infection by Toxoplasmosis and incidence of abortions in the studied areas. الخالصة متتاةدا ايةل تتل اتن لداةتة لدايدةتتل لداداالرلتل دتترل لداةتة لديدلاتتم لتا ةاتتة لدااتة عالاتل ر ألتذ63 لاترأة ابتةةل دت50 اتة لدر لرةتتل لديةدلتل دديرالتتر ل ادمتةر لدابت ا مبتةةة دذدت اتن لتفح ليت2007 يل) د ارة بالن مهر كتةادن لداتةاا داهةلتل متهر ينيترلن لدافيدبتل كةات لدر لرةتتل لديةدلتتل عتتر دجتتدر ةلاتتل ااادلتتل لدتدفنن لدتراد ب اتن بتالن لداالاتة ب بالا ت% 79.3 د اةتتةل بتتةةل داةدمفى لديرال لداتة ة رييتل ليت أجريت ,لدةبرة(لديرال لديدلام لدارلجاة ديرالتتر ة اتتن لتتفح يا هتتة دمجةتتة لدااتتةرة دتترل لدايدةتتة ااة الؤكر عتر دتياالر عاتر لداترأة لديةاتم لتا,د نيةرة لد فلفل لديةب ل لا اةةل لمبةةل اع دير لديدلام ةةداار لا لدفئل لدااريل لداةدال لدررلةل لديةدلل ل ردةتة لدداالت بتالن دجتدر لمبتةةل بترل لدايدةتل لداداالرلتل دترل لداةتة لديدلاتم كاة أداي,لدارردةل اةب لمبةةل ةةدارة لا لدااة ديردث لألجهةظ عار لديدلاتم ااتة لا تا اؤمت لر دلاتية ع تى ردر لد فال تا لداتذكدر لتا يترلث د ت لألجهةاتة لدررلةل دايةل ل لدةبرةب لا ااة Introduction Toxoplasmosis is a zoonotic disease caused by intracellular coccidian parasites, Toxoplasama gondii (Reiter-Owona, 2005; Song et al., 2005), it infect at least 500 millions people worldwide (Feron et al., 2001), and developed several potential routes of transmission within and between different host species (Cabanacan-Salibay and Claveria, 2006) such as swine (Mendonca et al., 2004) , Rodents (Kuticic et al., 2005), Sheep (Oncel and Vural, 2006) and Goats (Karaca et al., 2007). The definitive hosts of the parasite are domestic cats and other felines (Oncel et al., 2005). Infection is acquired by ingestion the sporulated oocyst excreted by cats that contaminate food and water and via ingestion of bradyzoites in the tissue of numerous food animals.(Oncel and Vural, 2006). Congenital toxoplasmosis results from the transplacental transmission of the parasite to the fetus (Wallon et al., 2004) causing various degree of damage, depending on the virulence of the parasites, the immune response of the mother and on the pregnancy period of the women when infected, resulting in fetal death or in severe clinical symptoms (Lopez et al., 2007). Primary infection with T.gondii in pregnancy is usually asymptomatic, nevertheless infection may be transmitted to the fetus (Morris and Croxson, 2004), and can cause severe damage such as microcephaly, retinochoroditis and cerebral calcification (Falavigna et al., 2007) especially if a women get infection for the first time in her life (Al-Harthi et al., 2006). Infections occurring in the first trimester have a 10% chance of transmission to the fetus and have the worst prognosis because of the risk of extensive central nervous system involvement, while the infection occurring in the later pregnancy is transmitted to the fetus more frequently but does not cause such extensive damage (Morris and Croxson, 2004). Toxoplasmosis is diagnosed in laboratory by immunological testing that give the titer of circulating antibodies, and in fetus toxoplasmosis based on direct identification of the parasite by inoculation of amniotic liquid and /or fetal blood in mice, as well as cell culture (Lopez et al., 2007). The seroprevalence of pregnant toxoplasmosis were reported in many countries as reaching 81% in Brazil, 71% in France, 42% in Italy, 37% in Slovenia, 47.1% in Jordan, 66.9 % in Kuwait, 43.4% in Saudi Arabia (Mendonca et al., 2004; Jumaian, 2005; Iqbal and Khalid, 2007). In Iraq little studies were carried out about pregnant toxoplasmosis.The aim of the present study to investigate the seroprevalence of T. gondii antibodies among pregnant women in North Basrah and discuss some factors associated with infections. Materials and Methods A total of 63 pregnant women was examined by collect a blood sample for the period from January to June 2007 in Al-Qurna hospital to determine Toxoplasma gondii antibodies. One sample for each pregnant was collected in tubes (5 ml), stored in container at 4C°. The sera was separated by centrifugation at 4000 rpm for ten minutes in room temperature, stored at -20 until use. The indirect haemagglutination test (IHAT) (BioKit, Barcelona, Spain) were used for serodiagnosis (IgM, IgG) of toxoplasmosis at dilutions 1:16-1:256. SPSS programme were used for statistical analysis. Results Out of 63 blood samples that collected from pregnant women in present study, 50 (79.3 %) were seropositive for Toxoplasma gondii in Al-Qurna hospital at titres 1:16-1:256. The seroposivity was distributed in different dilutions: 3 (7.5 %) at 1:16 dilution, 16 (40 %) at 1:32 dilution, 7 (17.5 %) at 1:64 dilutions, 16 (40 %) at 1:128 dilutions and 8 (20 %) at 1:256 dilutions. There is no significant differences in infection percentage between age groups of pregnant( P<0.05), although there is clearly decrease in this percentage in (20-29 years) group. Table 1. Table (1): Distribution of toxoplasmosis at age groups Age groups(Years) Examined Infected Percentage % 20> 20-29 30-39 11 9 81.8 35 17 27 14 77.1 82.3 Our results show that the seroposivity is gradually increase with number of previous abortions. The pregnant with two and three abortions had a highest percentage of toxoplasmosis antibodies. (Table 2) Table (2): Toxoplasmosis compared with previous abortions. No. Abortions None Examined Infected Percentage % 7 4 57.1 1 44 34 77.2 2 2 2 100 3 2 2 100 Discussion The importance of infection with Toxoplasma gondii is acquired by causing fetal infection if its acquired during pregnancy, with unpredictable manifestations in the fetus and neonatal and it can cause morbidity and mortality in immunocompromised patients(Foulon et al., 2000; Razzak et al., 2005). High percentage (79.3 %) were obtained in T. gondii antibodies in pregnant women of Basrah city compared with those in other locations of Iraq, these result can be attributed to that the Basrah city has a warm, moist climate which is regarded a favorable condition to longer viability for T.gondii oocyst. This suggest is supported by Remington et al., 2001; Dupouy-Camet et al., 2003; Hatam et al., 2005 who reported regional variations in the incidence of T.gondii infection rate from one country to another or even within the same country because climate, cultural differences regarding hygienic and feeding habits. No significant differences has been recorded at seroprevalence of toxoplasmosis in three age groups that showed in table (1). The suspected reason for this is the equal degree of exposing to causing agent (oocyst) of toxoplasmosis between the previous age groups. The present result is differ from most of studies carried out in Iraq and in the world which showed that the seroposivity of T.gondii increase with older age (AlHamdani and Mahdi, 1997; Song et al., 2005; Al-Harthi et al., 2006 and Yacoub et al., 2006). The increasing of infection with pregnant age is discussed by Jumaian (2005) that associated with soil exposure, which is greatest during childhood years, the principal mechanism by which people are exposed to T. gondii. The gradually increase of seropositivity with number of previous abortions shows the closely correlation between infection by T.gondii as a major causative agent of abortions in pregnant women in Southern Iraq. The prevention of toxoplasmosis including education of women at childbearing age about minimizing their risk for infection. Educational interventions assume that increase knowledge results in awareness. Health managers should emphasize the importance to avoiding raw or undercooked meat, handling raw meat safely and preventing close contact with cats (Foulon et al., 1994). References Al-Hamdani, M.M. and Mahdi, N.K.(1997). Toxoplasmosis among women with habitual abortion. Eastern Mediterranean Health j., 3(2): 310-315. 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