Download Strawberry DNA Extraction Lab

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts

DNA replication wikipedia , lookup

DNA repair wikipedia , lookup

Replisome wikipedia , lookup

DNA polymerase wikipedia , lookup

DNA nanotechnology wikipedia , lookup

DNA profiling wikipedia , lookup

Microsatellite wikipedia , lookup

United Kingdom National DNA Database wikipedia , lookup

Helitron (biology) wikipedia , lookup

Transcript
Name: ______________________________________________
Hr: _________
Strawberry DNA Extraction
DIRECTIONS:
1) Place 2 strawberries in a Ziploc bag. Close & Push all of the
air out when you seal it.
2) Mash the strawberries, in the bag, for about 2min. DO NOT
BUST THE BAG!
3) Pour 10mL of the Extraction Buffer into the bag & Reseal.
4) Mash the mixture for another minute.
5) Pour your mixture/solution into your coffee filter (in your funnel, which sits in the beaker).
6) Wait until all of the liquid has filtered through.
7) Pour your filtered liquid into a test tube.
8) Holding the test tube at an angle.
9) Add 5pipette-fuls of cold Rubbing Alcohol to the test tube. BE
CAREFUL: Make sure to add it down the side of the tube. DO
NOT MIX!
10) Let this sit for a few minutes. You should see DNA (looks like
snot) rise out of the solution.
DRAW:
- Draw your test tube, and all of the contents in side.
- Label all of the contents in your drawing.
QUESTIONS:
1) What is the function of DNA?
_______________________________________________________________________________
_______________________________________________________________________________
2) What did mashing the strawberries, and mixing them with soap, do to their cells?
_______________________________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
3) Which liquid (from the experiment) was DNA not soluble in? (THINK: What does soluble
mean? Soluble = Dissolve!)
_______________________________________________________________________________
_______________________________________________________________________________
4) Which part of the strawberry cells, did the DNA come out of?
_______________________________________________________________________________
_______________________________________________________________________________