Download 征文要求

yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts

Clinical neurochemistry wikipedia , lookup

Mitochondrial replacement therapy wikipedia , lookup

附件 1:征文要求
功能 4 个专题,投稿时请注明所属专题。摘要论点明确、叙述清楚、文字精炼,
限 800 字以内,包括题目、作者及单位、E-Mail 地址。要求使用英文撰写,所有
字体为 Times New Roman;标题为四号字加粗,署名小四号字,单位小五号字,
3、请提供能反映您成就的个人资料(不超过 500 字)及近期照片。
[email protected]。投稿截止日期:2014 年 10 月 31 日。
Decreased mitochondrial DNA copy number in the hippocampus
and peripheral blood during opiate addiction is mediated by
autophagy and can be salvaged by melatonin
Yue-Mei Feng,1,2 Yun-Fang Jia,1,2 Ling-Yan Su,1,2 Dong Wang,1,2 Li Lv,1,2 Lin
Xu1,* Yong-Gang Yao1,*
Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences &
Yunnan Province; Kunming Institute of Zoology; Kunming, Yunnan China; 2University of Chinese Academy of
Sciences; Beijing, China
Abstract:Drug addiction is a chronic brain disease that is a serious social problem and
causes enormous financial burden. Because mitochondrial abnormalities have been associated
with opiate addiction, we examined the effect of morphine on mtDNA levels in rat and mouse
models of addiction and in cultured cells. We found that mtDNA copy number was significantly
reduced in the hippocampus and peripheral blood of morphine-addicted rats and mice compared
with control animals. Concordantly, decreased mtDNA copy number and elevated mtDNA damage
were observed in the peripheral blood from opiate-addicted patients, indicating detrimental effects
of drug abuse and stress. In cultured rat pheochromocytoma (PC12) cells and mouse neurons,
morphine treatment caused many mitochondrial defects, including a reduction in mtDNA copy
number that was mediated by autophagy. Knockdown of the Atg7 gene was able to counteract the
loss of mtDNA copy number induced by morphine. The mitochondria-targeted antioxidant
melatonin restored mtDNA content and neuronal outgrowth and prevented the increase in
autophagy upon morphine treatment. In mice, coadministration of melatonin with morphine
ameliorated morphine-induced behavioral sensitization, analgesic tolerance and mtDNA content
reduction. During drug withdrawal in opiate-addicted patients and improvement of protracted
abstinence syndrome, we observed an increase of serum melatonin level. Taken together, our study
indicates that opioid addiction is associated with mtDNA copy number reduction and
neurostructural remodeling. These effects appear to be mediated by autophagy and can be
salvaged by melatonin.
Keywords: addiction, morphine, mitochondrial dysfunction, autophagy, melatonin
* Corresponding Author, E-mail: [email protected] or [email protected]
Prof. Lin Xu, Principle Investigator and Director, Key Lab of Animal
Models and Human Disease Mechanisms, Kunming Institute of
Zoology, Chinese Academy of Sciences. The research is mainly focused on
the fundamental basis of neuronal information encoding, storage and
retrieval and the underlying cellular and molecular mechanisms; building
animal models to investigate the pathogenesis of a series of psychiatry
disorders including drug addiction, depression, autism, Schizophrenia and
AD; utilizing the natural plant recourses to develop novel drugs for the
treatment of anti-psychiatry disorders. More than 60 papers were published in these high-ranked
journals such as Nature, PNAS, Neuron, Cell and Biol Psychiatry. Won rewards including
“Tan-jiazheng life scientific innovation prize”, “973 special advanced individual” etc. and applied
a number of domestic and foreign patents. More than 30 postgraduated students got MSc or PhD.