Download BI 200 - Exam #3A

BI 200 - Exam #3
Spring 2003
Name
Lab Section
Disclaimer
Consider each question, and answer each in the appropriate format (e.g., True or False).
You may qualify your answer if you have reservations. If your comments have merit,
you may receive partial or full credit.
Multiple Choice - 2 points each
1. Which of the following organisms is a photosynthetic archaea?
a.
b.
c.
d.
Oscillatoria
Chromatium
Euglena
Halobacterium
2. Which of the following phototrophs make ATP via substrate level phosphorylation?
a.
b.
c.
d.
e.
Archaea
Anoxygenic
Oxygenic
All of the above
None of the above
3. Which pigment is associated with archaean photosynthesis?
a.
b.
c.
d.
Bacteriorhodopsin
Chlorophyll
Carotenoid
Phycobillins
4. An external electron donor for the reduction of NADP to NADPH in the green nonsulfur bacteria is
a.
b.
c.
d.
H2O
H2SO4
H2
H2S
5. Oxygenic photosynthesis is also known as
a.
b.
c.
d.
Cyclic photosynthesis
Non-cyclic photosynthesis
Photosystem I
Gametogenesis
6. Chlorophyll structure includes all of the following except
a.
b.
c.
d.
Polypeptide
Phytol
Magnesium
Tetrapyrole ring structure
7. The highly folded inner thyllakoid membranes of cyanobacteria and chloroplasts are
stacked in disc like structures called
a.
b.
c.
d.
mitochondria
stroma
the matrix
grana
8. Cytochrome structure includes all of the following except
a.
b.
c.
d.
Polypeptide
Phytol
Iron
Tetrapyrole ring structure
9. Cyanobacteria form what differentiated cell type to carry out nitrogen fixation
a.
b.
c.
d.
spores
heterocysts
stromatolites
carboxysomes
10. How many quanta, or units of light energy must be absorbed for oxygenic
photosynthesis?
a.
b.
c.
d.
zero
one
two
three
11. Which of the following is not involved in prokaryotic transcription?
a. sigma factor
b. introns
c. the promotor
d. the operator region
12. The number of nucleotide bases on a strand of DNA required to code for a
polypeptide consisting of 150 amino acids would be:
a. 50
b. 150
c. 300
d. 450
e. 600
13. In conjugation genes on plasmids are transferred in matings between:
a.
b.
c.
d.
e.
two F- strains
F+ and F- cells
two F+ strains
a and b
b and c
14. The type of bacterial virus which is covalently inserted into its host’s DNA is called
a.
b.
c.
d.
bacteriophage
temperate phage
macrophage
virulent phage
15. Of the following, the earliest of these proteins expressed during a lytic infection is
a.
b.
c.
d.
capsomere
tail fiber protein
lysozyme
DNA polymerase
16. When DNA or RNA is being synthesized the next nucleotide is added to the ______
of the existing chain
a.
b.
c.
d.
e.
5’ phosphate
3’ phosphate
2’ hydrogen
3’ hydroxyl
5’ hydroxyl
17. Proteins interact predominantly within which portion of the DNA molecule?
a. major groove
b. minor groove
c. supercoil
d. 4’ carbon
18. Supercoiling in prokaryotes is typically introduced by ______________ blank which
creates _________________ breaks in the DNA
a.
b.
c.
d.
Topoisomerase I; single stranded
Topoisomerase I; double stranded
Topoisomerase II; single stranded
Topoisomerase II; double stranded
19. AT-rich DNA will melt
a. at a higher temperature than GC-rich DNA
b. at a lower temperature than GC-rich DNA
c. at the same temperature as GC-rich DNA
20. Chlorophyll a is green because it
a. absorbs green light and reflects red and blue light
b. absorbs red and blue light and reflects green light
c. absorbs red and blue light and fluoresces green light
d. absorbs green light and what it reflects is irrelevant
21. Viruses may be as small as
a. 0.02 nm
b. 0.02 m
c. 0.02 mm
d. 0.02 inches
22. The virus that poses the largest health threat to human beings, in terms of number of
people that have died since the turn of the century, is
a.
b.
c.
d.
e.
HIV
rhabdovirus
orthomyxovirus
Ebola virus
T4
23. Which part of RNA polymerase in prokaryotes is responsible for recognizing the
promotor region
a.
b.
c.
d.
 alpha
 beta
 sigma
core
24. If a bacterial chromosome contains 30% adenine bases, what will be the percent of
guanine?
a.
b.
c.
d.
0%
20%
30%
40%
25. Which of the following types of photosynthetic microbes carries out anoxygenic
photosynthesis?
a.
b.
c.
d.
cyanobacteria - Anabena
archaebacteria - Halobacterium
nonsulfur purple bacteria - Rhodopseudomonas
sulfate reducing bacteria - Desulfovibrio
4 secret free points
True or False (1 point each) Place the whole word True or the whole word False before
each of the following statements indicating their validity
___________ Transcription and translation are said to be coupled in prokaryotes.
___________ Eukaryotic genes contain non-coding regions called exons.
___________ Transcription is carried out by ribosomes
___________ RNA processing involves removal of 5’caps and 3’tails.
___________ Supercoiling in prokaryotes does not involve histone protein
___________ The lacZ gene codes for Lactose Permease
___________ Mutations are errors during translation
___________ Codons are normally larger than transposons
___________ Introns occur in prokaryotic genes.
___________ A theta structure occurs in a partially replicated eukaryotic chromosome.
___________ Complementary strands of DNA are identical in base composition
___________ The membrane of an enveloped virus is taken from the host cell
___________ BI 200 (micro) is harder than BO 200 or ZO 200
___________ BI 200 is more fun than BO 200 or ZO 200
10 points - In studying several unusual organisms found on a meteorite from Mars you
are able to sequence their entire genomes. Given the information below indicate if each
is most likely prokaryotic, eukaryotic, or virus, and name any genetic elements indicated.
Organism 1 – 6,800 bases of single stranded RNA.
Organism 2 – 1.4 x 106 base pairs of double stranded DNA. There is a single copy of this
element, and it is circular. There is an average of 20 copies of a smaller element, 8,600
base pairs of DNA, and it is also circular.
Organism 3 – 4 x 108 base pairs of DNA distributed among 3 pairs of linear pieces of
DNA. Within the sequence, there is a 300 base segment that is repeated 100,000 times.
Organism 4 – 3 x 107 base pairs of DNA distributed among 4 pairs of linear pieces of
DNA. There is another genetic element of 10,000 base pairs of linear DNA. There is
also another genetic element that is a sequence of 1200 base pairs of DNA and occurs
twice in the chromosome, and once in the smaller linear piece of DNA.
Organism 5 – 1.8 x 106 base pairs of DNA found in a single linear chromosome. There is
one copy per cell. Some cells have an additional element of 48,000 base pairs always
inserted in the same spot in that chromosome.
1 point each. Fill in the blank from the
viruses listed on this page. There may
be more than one correct answer, and
you may use an answer more than once,
but put only one answer for each.
_________________________________
A sexually transmitted disease that can
cause cervical cancer
_________________________________
Any bacteriophage
_________________________________
Exhibits an unusually wide range of
vertebrate hosts
_________________________________
Any icosohedral virus
_________________________________
Any disease caused by a prion
_________________________________
Any helical virus
_________________________________
Your favorite virus
_________________________________
Any enveloped virus
_________________________________
Your least favorite virus
_________________________________
Any complex virus
_________________________________
HIV is this type of virus
_________________________________
A temperate bacteriophage
_________________________________
Causes a lytic infection in bacteria
_________________________________
Causes a lytic infection in people
_________________________________
Poliovirus is an example of this type of
virus
_________________________________
Causes a persistant infection
_________________________________
The type of virus that causes SARS is
thought to be
_________________________________
Causes small pox
, Beta
, lambda
Adenovirus
BSE
Chronic wasting disease
Corona virus
Creutzfeldt-Jakob
Ebola virus
Hepatitis A
Hepatitis B
Herpesvirus
HIV
kuru
Lambda
M13
Mu
Orthomyxovirus
Papilloma virus (HPV)
Parvovirus
Picornavirus
Poxvirus
Retrovirus
Rhabdovirus
Rhinovirus
scrapie
T4
Diagram (10 points). Make a diagram of the coupled transcription and translation of the lac operon. Indicate the DNA, the lacZ, lacY,
and lacA genes, RNA polymerase, polycistronic mRNA, ribosomes, and newly formed proteins. Also indicate the promoter, operator,
where the RNA polymerase binds. This is also known as the “Christmas tree” structure, or polysome.