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1 High Throughput Screening Laboratory High Throughput Screening Proposal (Fill in information that is available) Instructions: Please fill information in Section 1 and return to the KU-HTS contact Melinda Broward ([email protected]). Information in Section 2 will be completed in meeting to be scheduled with Principal Investigator and KU-HTS personnel. Principal Investigator Information Name: _______________________________________________________________________________ Institution: ____________________________________________________________________________ Department/Lab: _______________________________________________________________________ Screen Charge to information (charge code or bill to information): _____________________________________________________________________________________ E-mail:_______________________________________________________________________________ Phone #:______________________________________________________________________________ Fax #:________________________________________________________________________________ Personnel working on project (name and position):_____________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ Biology, Rationale, and Purpose Screen Indicate if looking for a drug candidate or for a biochemical tool._________________________________ Biological event measuring (circle): protein-protein binding, enzymatic activity modulation, cell toxicity or other. Provide a brief description of the biology, rationale, and goals for conducting the screen. _____________________________________________________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ Add any data that has been generated to support scientific validity of proposed assay (statistical experimental design, enzyme kinetic analysis, receptor/ligand K0 determinations, cell growth characteristics, etc.). _________________________________________________________________________________ _____________________________________________________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ Assay Protocol If commercial kits are available provide brief description. ____________________________________________________________________________________ ____________________________________________________________________________________ Manufacturer name _______________________________ and catalog # _________________________ Confidential 2 Assay type: homogeneous / cell-based or other Assay format: 96-well / 384-well / other Positive/Negative controls: _______________________________________________________________ Working concentrations or modulating conditions for your assay _______________________________________________________________________________________ ___________________________________________________________________________________ Activation/Inhibition of Target (Positive or negative): ___________________________________________ _____________________________________________________________________________________ Are there any safety concerns associated with assay? __________________________________________ _____________________________________________________________________________________ Readout mode (fluorescence / luminescence / absorbance): Readout wavelength(s): _________________________________________________________________ Assay Sensitivity and Specificity _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ Cell Based Assays: Cell line and Origin of Cells Transfected gene if any Do you need clone selection and optimization services? Scale up cell culture procedure Cell seeding process Cell passage effects Number of cells per well, density Cell harvesting process Assay Signal Stability Special Requirements Proposed plate map with controls Process flow chart Reagents and medias Protein Based Assays: Description Sufficient enzyme/protein for entire screen Any batch or differences Enzyme/substrate stability Enzyme/substrate storage conditions Enzyme/substrate freeze-thaw stability Assay Signal Stability Special Requirements Proposed plate map with controls Process flow chart Reagents and medias Confidential 3 (Insert additional details of procedures) _____________________________________________________________________________________ _____________________________________________________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ _____________________________________________________________________________________ Screening Timetable and Logistics Which libraries do you anticipate screening (Circle)? Validation, Large Collection (ChemBridge/ChemDiv), Focused, or Natural Product libraries Is assay still being optimized?_____________________________________________________________ Suggested equipment and any special requirements. List equipment and readers currently being used. ________________________________________________________________________________ ___________________________________________________________________________________ Timing issues, potential stop points_________________________________________________________ Stability and Process Studies______________________________________________________________ Reagent storage requirements and stability for projected duration of HTS work_______________________ _____________________________________________________________________________________ Reaction stability over projected assay time___________________________________________________ _____________________________________________________________________________________ Are reagents and supplies readily available, any necessary order lead times or production times? _____________________________________________________________________________________ Proposed plate map and Incubation time (with compound): _____________________________________________________________________________________ Provide a realistic estimate as to when assay will be ready for validation screening at HTS _______________________________________________________________________________________ __________________________________________________________________________________ Technology Transfer and Intellectual Property (IP) Have you talked to the Technology Transfer office about potential intellectual property associated with you biological target or assay method? _________________________________________________________ Has a provisional patent been filed (date filed or contacted office)? ________________________________ Do you have an upcoming presentation or publication disclosing IP? ______________________________ _______________________________________________________________________________________ ___________________________________________________________________________________ Data Handling Do you need raw data only or data analyzed:________________________________________________ Do you want the data to be kept private or open to the KU HTS community (once IP protected and you have published). Yes or No; if yes, then you will get to see other shared data and vice versa. Confidential
