Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Powerpoint - Blood Journal
Transcript
Sphingosine-1-phosphate and lysophosphatidic acid trigger invasion of primitive hematopoietic cells into stromal cell layers by Nobuaki Yanai, Naoko Matsui, Tadashi Furusawa, Tadashi Okubo, and Masuo Obinata Blood Volume 96(1):139-144 July 1, 2000 ©2000 by American Society of Hematology THS119 cells on TBR59 stromal cells.THS119 cells were maintained within the TBR59 layer as phase-contrast nonrefractile cells (A) and are also shown in cocultures 3 hours after passage with 5% FBS (B), without FBS (C), or with heat-inactivated 5% FBS (D). Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Invasion of THS119 cells into the TBR59 cell layers.The 2.5 × 106 THS119 cells were cultured on TBR59 cells for 3 hours at 33°C with or without supplements. Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Effect of several kinds of lipids on the ability of THS119 to invade TBR59 cell layers.The 2.5 × 106 THS119 cells were cultured on TBR59 cell layers for 3 hours at 33°C with or without 5 μg/mL of indicated lipids. Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Effect of PTx and C3 exotoxin on the ability of THS119 cells to invade TBR59 cell layers.SLO permeabilized 2.5 × 106 THS119 cells were cultured on TBR59 cell layers for 3 hours at 33°C. Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Expression of edg-1 and edg-2 by parental THS119 cells and cytokine-dependent THS119 sublines.Total RNA (0.5 μg) from bone marrow, THS119, stroma-independent IL-3–dependent THS119 subline (THS119IL-3), and stroma-independent IL-7–dependent THS119 subline (T... Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Expression of edg-1 and edg-2 in long-term bone marrow culture cells.Cells were obtained from the culture medium (floating hematopoietic cells), or were collected after enzyme treatment, followed by further short-term incubation to separate the stromal cell... Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Expression of edg-1 and edg-2 in lineage marker–negative hematopoietic cells from bone marrow.Total RNA from 4000 cells of Sca-1−/c-Kit−, Sca-1+/c-Kit−, Sca-1−/c-Kit+, Sca-1+/c-Kit+, and THS119 cells were analyzed by RT-PCR. edg-1, 442 bp fragment specific ... Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology Nobuaki Yanai et al. Blood 2000;96:139-144 ©2000 by American Society of Hematology
