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Interferon-γ–Dependent Inducible Expression of the Human Interleukin-12 p35 Gene in Monocytes Initiates From a TATA-Containing Promoter Distinct From the CpGRich Promoter Active in Epstein-Barr Virus-Transformed Lymphoblastoid Cells by Mark P. Hayes, Finbarr J. Murphy, and Parris R. Burd Blood Volume 91(12):4645-4651 June 15, 1998 ©1998 by American Society of Hematology Structure of the human IL-12 p35 gene (A and B). Mark P. Hayes et al. Blood 1998;91:4645-4651 ©1998 by American Society of Hematology Steady-state p35 mRNA induced in monocytes is approximately 0.2 kb shorter than constitutive or enhanced mRNA in EBV-transformed lymphoblastoid cells (Northern analysis). Mark P. Hayes et al. Blood 1998;91:4645-4651 ©1998 by American Society of Hematology RNase protection analysis using an antisense riboprobe (420 bases) from the 5′ end of the CLMF cDNA results in smaller protected fragments of 293-297 bases from induced monocyte mRNA. Mark P. Hayes et al. Blood 1998;91:4645-4651 ©1998 by American Society of Hematology Map of transcription start sites in the human IL-12 p35 promoter. Mark P. Hayes et al. Blood 1998;91:4645-4651 ©1998 by American Society of Hematology 5′ RACE analysis of monocyte RNA yields a single amplification product, not detectable from VDS RNA, that maps to a unique transcription start site. Mark P. Hayes et al. Blood 1998;91:4645-4651 ©1998 by American Society of Hematology
