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Download Effect of anti-vWf monoclonal antibodies on vWf binding to platelets
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Transcript
Structure and function of the von Willebrand factor A1 domain: analysis with monoclonal antibodies reveals distinct binding sites involved in recognition of the platelet membrane glycoprotein Ib-IX-V complex and ristocetindependent activation by Mariagrazia De Luca, David A. Facey, Emmanuel J. Favaloro, Mark S. Hertzberg, James C. Whisstock, Tracy McNally, Robert K. Andrews, and Michael C. Berndt Blood Volume 95(1):164-172 January 1, 2000 ©2000 by American Society of Hematology Mapping the epitope for the anti-vWf monoclonal antibody, 6G1.Inhibition of binding of 125Ilabeled monoclonal antibody 6G1 (final concentration, 1 μg/mL) to immobilized vWf by synthetic peptides (final concentration, 100 μM) in 30 minutes at 22°C. Mariagrazia De Luca et al. Blood 2000;95:164-172 ©2000 by American Society of Hematology Epitopes for anti-vWf monoclonal antibodies.Structure of the von Willebrand factor A1 domain based on the x-ray crystal coordinates25 with highlighted sequences representing epitopes for the anti-39/34-kd vWf fragment monoclonal antibodies determined using ... Mariagrazia De Luca et al. Blood 2000;95:164-172 ©2000 by American Society of Hematology Cross blocking of anti-vWf monoclonal antibodies.Binding of 125I-labeled monoclonal antibodies (final concentration, 1 μg/mL) to immobilized native vWf (A-D) or 39/34-kd vWf fragment (E-F) in 30 minutes at 22°C. Mariagrazia De Luca et al. Blood 2000;95:164-172 ©2000 by American Society of Hematology Effect of anti-vWf monoclonal antibodies on vWf binding to platelets.Antibodies (final concentration, 50 μg/mL) were included in assays measuring binding of 125I-labeled vWf (1 μg/mL) to GP Ib-IX-V on washed platelets induced by (A) botrocetin (25 μg/mL), (... Mariagrazia De Luca et al. Blood 2000;95:164-172 ©2000 by American Society of Hematology Effect of anti-vWf monoclonal antibodies on vWf binding to botrocetin or jaracetin.Antibodies (final concentration, 50 μg/mL) were included in assays measuring binding of 125I-labeled vWf (1 μg/mL) to (A) botrocetin-coated beads or (B) jaracetin-coated bead... Mariagrazia De Luca et al. Blood 2000;95:164-172 ©2000 by American Society of Hematology Effect of the anti-vWf monoclonal antibody 6G1 on binding of the 39/34-kd vWf fragment to platelets.(A) Specific binding of 125I-labeled 39/34-kd vWf fragment (final concentration, 1 μg/mL) to washed platelets (final concentration, 5 × 108/mL) in the presen... Mariagrazia De Luca et al. Blood 2000;95:164-172 ©2000 by American Society of Hematology
