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Download Muscle markers and regulatory factors in marine fish under different
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Arch. Tierz., Dummerstorf 51 (2008) Special Issue, 60 Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain D. GARCIA DE LA SERRANA, MARTA CODINA, ISABELL NAVARRO and JOAQUIM GUTIÉRREZ Muscle markers and regulatory factors in marine fish under different developmental stages and treatments (Marker für Muskelgewebe und regulative Faktoren beim Seefisch in Abhängigkeit von Behandlung und Entwicklungsstadium) The research of molecular markers for flesh quality and their relationship with muscle development in fish is still scarce. Myogenic precursors cells have an important role in muscle growth and development, participating in hypertrophic fibre growth, new fibre formation and fibre repair. Many studies are focused to characterize these precursor cells markers (c-met, Pax7, Sox8) and signalling pathways (Akt, MAPK, mTOR) that regulate their function. Also, the Myogenic Regulatory Factors (MRFs) may be useful to determine muscle status in terms of growth and quality in fish. We studied molecules as c-met, Akt and MAPK in two species of marine fish: Gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) cultured at different dietary conditions. We have also focused our attention on the MyoD2, Myogenin and Myostatin expression in sea bream. Our studies using western blot in muscle of fish treated with different diets, show that c-met follows the similar pattern than fish weight and size, being the expression higher in those animals with better growth. On the other hand, MAPK and AKT pathways do not show the same tendency and their expression could be more influenced by the post-feeding stage. By means of Real Time PCR, preliminary studies in primary culture of sea bream myocytes suggest high levels of MyoD2 in early stages of cell development (proliferation stages), while Myostatin seems to be not expressed along the culture. Further studies will be done to elucidate the implication of the different MRFs on muscle formation in sea bream, and to determine the possible effects on IGFs expression. Supported by AGL2004-06319-C02/ACU and AGL2006-03923/ACU from the Ministerio de Educación y Ciencia and XRAq from DIUE, Generalitat de Catalunya. Corresponding author: JOOAQUIM GUTIÉRREZ Dept. de Fisologia Facultat de Biologia Universitat de Barcelona Av. Diagonal 645 0828 Barcelona Spain email: [email protected]
