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Applied Precision Live Cell Heating Option Components The top piece is a custom heating plate that mounts directly to the sample holder plate (ceramic piece) on the OMX stage. Either slides, or a variety of sample holders will fit into the heating plate. The lower piece is the objective warming ring. Since the sample and objective are thermally isolated from the rest of the optics block and mechanics, only the sample and objective are brought up to temperature. These are the controllers (identical) for the heating plate and the objective warmer. OMX Live Cell Heating Components Page 1 of 3 Applied Precision This is a flowmeter (on the left) for the CO2 input. This requires premixed 5% CO2, or whatever gas mixture desired. On the right is the humidifier that the gas will flow through prior to coming into contact with the sample. The CO2 flows from the humidifier to this cover, which has a glass window in the top, and sits directly over the sample on the stage. OMX Live Cell Heating Components Page 2 of 3 Applied Precision These are a few examples of the sample holders that will be provided. These fit into the heating plate in the first picture, and are designed to transfer heat from the heating plate to the sample. These are for dishes (left) and chambered coverslips (right). OMX Live Cell Heating Components Page 3 of 3