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Thermo Scientific NuLight® Fluorescent Labeled Phosphoramidites Wide spectral range of fluorescence for numerous applications Thermo Scientific NuLight® phosphoramidites are high performance choices for fluorescent oligonucleotide labeling. NuLight phosphoramidites are specific for the addition of a fluorescent tag to the 5’ position of an oligonucleotide using standard synthesis and deprotection conditions. O O O O O O O HN O N O P OCH2CH2CN • High Performance Higher active concentration than trityl protected dyes O O FAM-6 O O O O O O HN DY547 and DY647 products compare in emission and absorbance maxima to commonly available dye phosphoramidites N O P OCH 2CH 2 CN Fluorescein No licensing required for research applications N • Growing Spectrum of Dye Options NuLight FAM-6 (520 nm emission) NuLight Fluorescein- contains 5- & 6- isomers (520 nm emission) NuLight DY547 (574 nm emission) NuLight DY647 (673 nm emission) NuLight DY780 (800 nm emission) R&D expertise in custom dye development O P O N+ CN N DY547 N O P N+ O CN N I- DY647 OCH 3 O • Over 20 Years Experience Manufacturing high quality phosphoramdites and oligonucleotide synthesis reagents for 20+ years O+ N ClO4 - N N C O P O N DY780 Thermo Scientific NuLight® Fluorescent Labeled Phosphoramidites Product Specifications NuLight FAM-6 Phosphoramidite HPLC Purity 31 P NMR Isomeric Purity λmax abs λmax em Molar Absorbance > 90% > 85% > 95% 494 nm 520 nm 75,000 M-1 cm-1 NuLight Fluorescein Phosphoramidite HPLC Purity 31 P NMR λmax abs λmax em Molar Absorbance > 90% > 85% 494 nm 520 nm 75,000 M-1 cm-1 NuLight DY547 Phosphoramidite HPLC Purity λmax abs λmax em Molar Absorbance > 90% 549 nm 563 nm 150,000 M-1 cm-1 NuLight DY647 Phosphoramidite HPLC Purity λmax abs λmax em Molar Absorbance > 85% 645 nm 663 nm 250,000 M-1 cm-1 NuLight DY780 Phosphoramidite λmax abs λmax em Molar Absorbance 770 nm 800 nm 60,000 M-1 cm-1 Ordering Information Related Products Product Name Product # NuLight FAM-6 Phosphoramidite 27-1608 NuLight Fluorescein Phosphoramidite 27-1606 Thermo Scientific DNA Phosphoramidites NuLight DY547 Phosphoramidite SY6332 Thermo Scientific TheraPure® RNA Phosphoramidites NuLight DY647 Phosphoramidite SY6334 NuLight DY780 Phosphoramidite SY6330 Thermo Scientific TheraPure® DNA Phosphoramidites Thermo Scientific RNA Phosphoramidites Thermo Scientific NuLight® NHS Esters Thermo Scientific RNA CPG Synthesis Supports For more information visit the web site at www.thermo.com/milwaukee Thermo Fisher Scientific (Milwaukee) LLC We develop integrative partnerships with customers across the globe to provide premium molecular biology reagents and innovative services. These products are used in a variety of applications to advance the development of biotechnology research, diagnostic and therapeutic applications. Tel: 877-886-7629 (toll-free) • Tel: 414-227-3600 • Fax: 414-227-3757 Technical Assistance E-mail: [email protected] • Internet: www.thermo.com/milwaukee © 2007 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. TheraPure® and NuLight™ are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries. MK0050A01