Download Thermo Scientific NuLight® Fluorescent Labeled Phosphoramidites

Thermo Scientific NuLight®
Fluorescent Labeled Phosphoramidites
Wide spectral range of fluorescence
for numerous applications
Thermo Scientific NuLight® phosphoramidites
are high performance choices for fluorescent
oligonucleotide labeling.
NuLight phosphoramidites are specific for the
addition of a fluorescent tag to the 5’ position of
an oligonucleotide using standard synthesis and
deprotection conditions.
O
O
O
O
O
O
O
HN
O
N
O
P
OCH2CH2CN
• High Performance
Higher active concentration than trityl
protected dyes
O
O
FAM-6
O
O
O
O
O
O
HN
DY547 and DY647 products compare in
emission and absorbance maxima to
commonly available dye phosphoramidites
N
O
P
OCH 2CH 2 CN
Fluorescein
No licensing required for research applications
N
• Growing Spectrum of Dye Options
NuLight FAM-6
(520 nm emission)
NuLight Fluorescein- contains 5- & 6- isomers
(520 nm emission)
NuLight DY547
(574 nm emission)
NuLight DY647
(673 nm emission)
NuLight DY780
(800 nm emission)
R&D expertise in custom dye development
O P
O
N+
CN
N
DY547
N
O P
N+
O
CN
N
I-
DY647
OCH 3
O
• Over 20 Years Experience
Manufacturing high quality
phosphoramdites and oligonucleotide synthesis reagents for 20+ years
O+
N
ClO4
-
N
N
C
O
P
O
N
DY780
Thermo Scientific NuLight®
Fluorescent Labeled Phosphoramidites
Product Specifications
NuLight FAM-6 Phosphoramidite
HPLC Purity
31
P NMR
Isomeric Purity
λmax abs
λmax em
Molar Absorbance
> 90%
> 85%
> 95%
494 nm
520 nm
75,000 M-1 cm-1
NuLight Fluorescein Phosphoramidite
HPLC Purity
31
P NMR
λmax abs
λmax em
Molar Absorbance
> 90%
> 85%
494 nm
520 nm
75,000 M-1 cm-1
NuLight DY547 Phosphoramidite
HPLC Purity λmax abs
λmax em
Molar Absorbance
> 90%
549 nm
563 nm
150,000 M-1 cm-1
NuLight DY647 Phosphoramidite
HPLC Purity
λmax abs
λmax em
Molar Absorbance
> 85%
645 nm
663 nm
250,000 M-1 cm-1
NuLight DY780 Phosphoramidite
λmax abs
λmax em
Molar Absorbance
770 nm
800 nm
60,000 M-1 cm-1
Ordering Information
Related Products
Product Name
Product #
NuLight FAM-6 Phosphoramidite
27-1608
NuLight Fluorescein Phosphoramidite 27-1606
Thermo Scientific DNA Phosphoramidites
NuLight DY547 Phosphoramidite
SY6332
Thermo Scientific TheraPure® RNA Phosphoramidites
NuLight DY647 Phosphoramidite
SY6334
NuLight DY780 Phosphoramidite SY6330
Thermo Scientific TheraPure® DNA Phosphoramidites
Thermo Scientific RNA Phosphoramidites
Thermo Scientific NuLight® NHS Esters
Thermo Scientific RNA CPG Synthesis Supports
For more information visit the web site at
www.thermo.com/milwaukee
Thermo Fisher Scientific (Milwaukee) LLC
We develop integrative partnerships with customers across the globe to provide premium molecular biology reagents and innovative
services. These products are used in a variety of applications to advance the development of biotechnology research, diagnostic and
therapeutic applications.
Tel: 877-886-7629 (toll-free) • Tel: 414-227-3600 • Fax: 414-227-3757
Technical Assistance E-mail: [email protected] • Internet: www.thermo.com/milwaukee
© 2007 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing
applications only. TheraPure® and NuLight™ are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
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