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Hellenic Accreditation System S.A. Annex F1/10 to the Certificate No. 213-4 SCOPE of ACCREDITATION of the Central Public Health Laboratory of the KEELPNO of the Ministry of Health Materials/ Products tested Types of test/ Properties measured Applied standards/ Techniques used Microbiological Tests 1.Enumeration of culturable microorganisms –colony count by inoculation in a nutrient agar culture medium at 222 0C and at 362 0C ΕLΟΤ ΕΝ ISO 6222:2000 2.Detection and enumeration of Pseudomonas aeruginosa ELOT ΕΝ ISO 16266:2009 2.Water for human consumption, natural mineral water, surface waters intended for human consumption 1.Detection and enumeration of Clostridium perfringens (spores included) Annex III of the J.M.D. Υ2/2600/2001 as modified, completed and valid until now 3.Water for human consumption, natural mineral water, surface waters, sea water, pool water and recreational waters 1.Detection and enumeration of Escherichia coli and coliform bacteria ΕLΟΤ ΕΝ ISO 9308-1: 2001 2.Detection and enumeration of intestinal enterococci ΕLΟΤ ΕΝ ISO 7899-2: 2001 4.Water for human consumption, pool water, recreational waters and other types of water 1.Detection and enumeration of Legionella spp. ISO 11731:1998 2.Detection and enumeration of Legionella spp. – Part 2: Direct membrane filtration method for waters with low bacterial counts ISO 11731-2:2004 1.Water for human consumption, natural mineral water, surface water, pool water and recreational waters Page 1 of 3 Annex F1/10 to the Certificate No. 213-4 06.06.2013 Materials/ Products tested 5.Food 6.Clinical and environmental Salmonella spp strains 7.Clinical and environmental strains of other Enterobacteriaceae Types of test/ Properties measured Applied standards/ Techniques used 1.Detection of Salmonella spp. ΕLΟΤ EN ISO 6579:2003 2.Detection of Listeria monocytogenes ΕLΟΤ ΕΝ ISO 11290.01: 1997 /Amd1:2004 3.Enumeration of Listeria monocytogenes ΕLΟΤ ΕΝ ISO 11290.02: 1999 /Amd1:2004 4.Detection of E. coli Ο157:Η7 ΕLΟΤ ΕΝ ISO 16654:2001 5.Enumeration of coagulase-positive Staphylococci (Staphylococcus aureus and other species) ΕLΟΤ ΕΝ ISO 6888-1:1999 /Αmd1:2004 1.Serotyping of Salmonella spp. Slide Agglutination, White Kauffmann - Le Minor Scheme_2007, 9th edition 2.Antimicrobial resistance of Salmonella spp. in : Ampicillin, Cefotaxime, Amoxicillin + clavulanic acid, Ceftazidime, Chloramphenicol, Tetracycline, Trimethoprim, Nalidixic, Amikacin, Ciprofloxacin, Streptomycin, Kanamycin, Netilmicin, Tobramycin Antimicrobial Disk Susceptibility Tests CLSI:Μ2-Α10, Vol. 29, No. 1 CLSI:M100- S21, Vol.31 No. 1 1.Antimicrobial resistance of Enterobacteriaceae in: Ampicillin, Amoxicillin +clavulanic acid, Piperacillin, Piperacillin + tazobactam, Ticarcillin,Ticarcillin + clavulanic acid, Cefoxitin, Cephalothin, Cefotaxime, Ceftazidime, Cefepime, Aztreonam, Imipenem, Meropenem, Ertapenem, Doripenem, Tetracycline, Sulphamethoxazole + Trimethoprim, Amikacin, Gentamicin, Ciprofloxacin Antimicrobial Disk Susceptibility Tests CLSI:Μ2- Α10, Vol. 29, No. 1 CLSI:M100- S21, Vol.31 No. 1 Chemical Τests 1.Water for human consumption, natural mineral water, surface waters and pool water 2.Water for human consumption, natural mineral water, surface waters and wastewater Page 2 of 3 1. Determination of total hardness APHA* 2340 C 2. Determination of calcium APHA* 3500 - Ca B 3. Determination of magnesium APHA* 3500 - Mg B 4. Determination of chloride ions APHA* 4500 - Cl- B 5. Determination of alkalinity APHA* 2320 B 6. Determination of Total Dissolved Solids (TDS) dried at 180 °C APHA* 2540 C 1. Determination of Total Suspended Solids (TSS) dried at 103-105 °C APHA* 2540-D 2. Determination of pH APHA* 4500-H+ 3. Determination of conductivity APHA* 2510-B Annex F1/10 to the Certificate No. 213-4 06.06.2013 Materials/ Products tested 3.Wastewater Types of test/ Properties measured Applied standards/ Techniques used 1.Determination of Biochemical Oxygen Demand BOD 5 days In house Method (ΜΔ-111), based on APHA* 5210 D 2.Determination of Chemical Oxygen Demand COD In house Method (ΜΔ-110) based on APHA* 5220 D Molecular Methods 1.Clinical and environmental strains of Enterobacteriaceae 2.Clinical and environmental strains of Ε.coli (VTEC) 1. Bacterial typing In house method (ΜΔ-26) for the preparation, restriction enzyme digestion and PulsedField Gel Electrophoresis of agarose plugs 2. Detection of resistance genes blaVIM & blaKPC In house Method (ΜΔ-25) 1. Detection of toxin genes (vtx1 and vtx2) Polymerase Chain Reaction (PCR), based on the proposed protocol of WHO Collaborating Centre for Reference and Research on E. coli –Statens Serum Institute, EU Reference Laboratory VTEC Instituto Superiore di Sanita Polymerase Chain Reaction (PCR) * ΑPHA: American Public Health Association, American Water Works Association, Water Environment Federation, “Standard Methods for the Examination of Water and Wastewater”, 21st Edition, 2005 Site of assessement: Permanent Laboratory premises, 34 Fleming str., 16672, Vari, Attiki, Greece. Approved signatories: A. Vatopoulos, Em. Velonakis, A. Chaldoupi, G. Papageorgiou, I. Spiliopoulou, E. Mathioudaki, T. Karabinas, M. Kontarini, A. Flountzi, M. Lambiri, G. Mandilara, P. Giakkoupi, K. Trifinopoulou. This Scope of Accreditation replaces the previous one dated 21.02.2013. The Accreditation Certificate No. 213-4, to ELOT EN ISO/IEC 17025:2005, has been extended up to 21.09.2013. Athens, June 06, 2013 Ioannis Sitaras Director of the Laboratories Accreditation Division Page 3 of 3 Annex F1/10 to the Certificate No. 213-4 06.06.2013