Download APC Mouse Anti-Human Angiotensin Converting Enzyme (CD143

BD Pharmingen™
Technical Data Sheet
APC Mouse Anti-Human Angiotensin Converting Enzyme (CD143)
Product Information
557929
ACE1; DCP1; Kininase II
100 Tests
20 µl
BB9
Human bone marrow-derived stromal cells
Mouse IgG1, κ
QC Testing: Human
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Material Number:
Alternate Name:
Size:
Vol. per Test:
Clone:
Immunogen:
Isotype:
Reactivity:
Storage Buffer:
Description
The BB9 Monoclonal antibody specifically binds Angiotensin-converting enzyme (ACE) which is also known as Dipeptidyl carboxypeptidase
1 (DCP1) or CD143. CD143 is a type I transmembrane glycoprotein that functions as a zinc metallopeptidase involved with the metabolism of
angiotensin, bradykinin, and other oligopeptides. It is expressed on primitive hematopoietic progenitor cells (HPC). BB9 reacts with stromal
cells, a minor subset of human bone marrow cells; it does not react with peripheral blood leucocytes. BB9 recognizes a subset of
CD34+CD90+ cells that demonstrate low or absent expression of CD38, low retention of Rhodamine 123 and are Ki-67-negative.
CD34+BB9+ cells are able to sustain hematopoiesis in pre-CFU culture stimulated with IL-3, IL-6, G-CSF, and SCF. BB9 also reacts with a
subset of CD34+ cells from mobilized peripheral blood. This antibody was generated using human bone marrow-derived stromal cells as
immunogen.
Flow cytometric analysis of CD143 expression on
human peripheral blood lymphocytes. Whole blood was
stained with either APC Mouse Anti-Human Angiotensin
Converting Enzyme (CD143) (Cat. No. 557929; solid line
histogram) or APC Mouse IgG1, κ Isotype Control (Cat. No.
555751; dashed line histogram). Erythrocytes were lysed
with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The
fluorescence histograms were derived from gated events
with the forward and side light-scatter characteristics of
viable lymphocytes.
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Application Notes
Application
Flow cytometry
Routinely Tested
Suggested Companion Products
Catalog Number
555751
554656
555899
557929 Rev. 5
Name
APC Mouse IgG1, κ Isotype Control
Stain Buffer (FBS)
Lysing Buffer
Size
100 Tests
500 mL
100 mL
Clone
MOPC-21
(none)
(none)
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Product Notices
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This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental
sample (a test).
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
www.bdbiosciences.com/colors.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
An isotype control should be used at the same concentration as the antibody of interest.
References
Jokubaitis VJ, Sinka L, Simmons PJ, et al. Angiotensin-converting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult
hematopoietic tissues. Blood. 2008; 111(8):4055-4063. (Clone-specific)
Ramshaw HS, Haylock D, Swart B, et al. Monoclonal antibody BB9 raised against bone marrow stromal cells identifies a cell-surface glycoprotein expressed by
primitive human hemopoietic progenitors. Exp Hematol. 2001; 29(8):981-992. (Immunogen)
Sinka L, Biasch K, Khazaal I, Péault B, Tavian M. Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human
embryo. Blood. 2012; 119(16):3712-3723. (Clone-specific)
Zambidis ET, Park TS, Péault B, et al. Expression of angiotensin-converting enzyme (CD143) identifies and regulates primitive hemangioblasts derived from
human pluripotent stem cells. Blood. 2008; 112(9):3601-3614. (Clone-specific)
557929 Rev. 5
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