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Download Fish model species in human and environmental toxicology Jessica
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Fishmodelspeciesinhumanandenvironmentaltoxicology JessicaLegradi,RiccardoMassei,MarekPípal May11,8:35-12:45,Hall400 Fishmodelsarecommonlyusedinhumanandeco-toxicitytestingtoinvestigatetheimpactof chemicalsonwholeorganisms.Manyimportantbiologicalfunctionsareconservedbetweenfish speciesandhumans.Thereforefishhaveawideutilitydomain,spanningfrombasicdevelopmental biology,neurobiology,endocrinologytoimmunology.Thesmallsizeofsomeavailablefishspecies includingthezebrafish(Daniorerio)ormedaka(Oryziaslatipes)andtheirrobustnaturemakesthem ideallysuitedforapplicationinautomatedhighthroughputscreens.Furthermore,earlylifestagesof thesespeciesofferallthekeyattributesofacomplexinvivosystem(e.g.includingmetabolism),as wellasattributesofinvitroassays,astestscanbecarriedoutinmultiwellplatesformatswithsmall samplevolumesandrunincomparativelyshortperiodsoftime.Theseattributesmakethemwell suitedfortoxicitytestingofenvironmentalextractsandineffectdirectedanalysis(EDA)todetect unknowncontaminantsincomplexsamples.Researchonfishoverthelastdecadehasbeengreatly facilitatedbytheavailabilityofsequencedgenomes,whichareavailableforovertwelvespecieswith morepending.Thisfacilitytogetherwithadvancesingeneticandepigeneticstudies,includinggene knockoutandtransgenesistechnologies,isgreatlyfacilitatingunderstandingofthemolecular mechanismsoftoxicology.Duetothelargesimilaritywithothervertebrates,thereisalsoagrowing interestintheapplicationoffishmodelspeciesinhumandiseaseanddevelopment.Fishearlylife stageshavebeenrecentlyusedinseveralcancergeneticsstudiesanddrugdiscoverytests.Inthe ecotoxfieldfisharealsostudiedoutsideofthelaboratoryintheirnativeenvironment.Prominent modelsfornativefishmodelsareroach(Rutilusrutilus)andrainbowtrout(Oncorhynchusmykiss). Studyingfishintheirnaturalhabitatallowstogofurtherthansimpledose-effectassessments.Within thissessionweintendtoshowrecentdevelopmentsintoxicologicalresearchusingavarietyof differentfishmodelspecies,focusingonnovelsystems,endpoints,assaysandtestingstrategies. Resultsoftoxicitystudiesofsinglecompoundsaswellascomplexenvironmentalsamplesareof interest.Effectsonindividualfish,multigenerationalexposureeffects,andpopulationlevelimpacts willbeconsidered.Weespeciallywelcomepresentationshighlightingnewanalyticalmethodsand techniquesforcontaminantsortheirmetabolitesinexposuremediaorfish.Thesessionwillbe interdisciplinaryandbringtogetherresearchersacrossawiderangeofresearchareaswiththeview toenhanceapproachesinhumanandecotoxicitytesting.
