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Pathogen Detection in Water Samples
A more recent approach for testing for aquatic
pathogens such as bacteria and myxozoan parasites
is water sampling. Water is collected either by hand
or with an automatic sampler. Samples are filtered,
the DNA is extracted and then molecular methods
such as quantitative PCR are used to determine the
identity and abundance of pathogens.
Water sample data are usually obtained
more quickly than sentinel fish exposure
data, but have a higher detection
threshold. We use a combined
approach of water sampling and
sentinel fish exposures to relate
abundance of waterborne stages with
fish health.