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Biological Diversity Field Study Task From where you are standing, how many different plants can you see? Stand quietly for at least 2 minutes. Record the number of birds that you see or hear. Name the different kinds of sounds that you hear? I am in a biologically diverse place. (Yes or No) I found a rock with living things on it or under it. (Yes or No) Find an animal with 6 legs. What is it? (Yes or No) I found an insect living in the soil. (Yes or No) I found an animal with no legs. (Yes or No) Find insects living in leaf litter. Name them. How many different types of plants are in a 5 foot square? Name a change made by humans that decreased biodiversity. Name a change made by humans that increased biodiversity. Answer Collect Leaf Litter Materials: Trowel Gloves Large collection bin and lid Collection tray Magnifying glass (or loupe) Tweezer Measuring tape String Golf tees Pencil Field journal Identification key Procedure: 1. Find a place in the school yard that has been relatively undisturbed by human activity. There should be fallen leaves, twigs, and pieces of bark. 2. Measure out a patch of leaf litter, one-yard square. Use the string and tees to build a rough sampling frame. Have you seen any animals in your sampling area so far? How many animals do you think you will find? 3. Put on your gloves and use the trowel to collect all the leaf litter and soil to about ½ inch depth within your sampling frame. 4. Put all the leaf litter in the large collection bin. Using your fingers (keep gloves on), spread out the leaf litter so an even layer is created. Keep your eye out for little critters! 5. Return to the classroom. 6. With your magnifying glass, examine the leaf litter for any worms, spiders or insects. Use your gloved fingers to gently sift through the litter. 7. Using tweezers, gently place any animals found in the litter into the collection bin’s lid. 8. Examine the small animals. How many legs are there, if any? Do they have an exoskeleton or a hard shell? Do their legs look jointed? What color are they? What shape are they? Are there different sized critters? Scientists use questions like these to categorize animals into different groups. 9. Record your data in the following lab record sheet. 10. Return all specimens and leaf litter to where you originally found them. Bring all sampling equipment back to the classroom. 11. Compare the results of your field study with others in your classroom. Species Evenness Sorting and Counting Litter Critters Species Richness: Total number of species Date: _______________________________ Species richness for my leaf litter plot: ________________ Describe the area where your litter critters were found. Species Evenness: How evenly distributed the species are in a designated community. Use the table to record your collected data. You do not have to identify the organism. You can name the organisms Beetle 1, Beetle 2, Beetle 3, Or Organism A, or Organism B, Organism C, etc. Species Name or Description Quantity Found Species Evenness = Number of individuals of one species found Total number of individuals found in a habitat Here is an example of the calculation for species evenness: Species Total # Found Beetle A Beetle B Worm A Total 25 50 10 85 Total # of Individuals 85 85 85 Species Evenness Ratio 25/85 = 0.294 50/85 = 0.588 10/85 = 0.118 1 Determine the species evenness of each individual species and record in the table below. Species Total Individuals Draw your litter critters. Total Total # Found Total # of Individuals Species Evenness Ratio Simpson’s Diversity Index Numerator The Simpson's Diversity Index is an index that represents a quantitative measurement of a habitat’s biodiversity. It takes into account the number of species present, as well as the species evenness. n(n -1) = Denominator N(N-1) = Procedure: Where: n = the total number of organisms of each individual species N = the total number of organisms of all species The value of D ranges from 0 to 1. With this index, 0 represents infinite diversity and, 1, no diversity. That is, the bigger the value the lower the diversity. Jelly Bean Community Species Total n (# individuals) N= n–1 1. Divide your species (jelly beans) by color into groups. 2. Name your species (green jelly bean, red jelly bean, etc.) and list them in the first column under Species. 3. Count how many of each species you have and note that number in the second “n” column. 4. Subtract 1 from each number in the “n” column and place it in the “n-1” column. a. i.e., 5 green jelly beans, 5-1=4 b. Multiply column “n” by column “n-1” i.e., 5x4 =20. Place that number in the last column “n(n-1).” n(n – 1) 5. After you’ve finished this calculation from each species, add up the numbers in the fourth column. n(n -1) = ??. This is your numerator (top number). 6. Count all of the jelly beans on your plate. That is your N number. (You can also add the number is the “n” column. 7. To get the denominator, multiply your “N” number by “N-1. 8. Divide. Your number should be less than 1. n(n -1) = Comparing Leaf Litter Samples 1. What kinds of organisms are most common in leaf litter? 2. Why are leaf litter organisms important? 10. What would happen if the organisms that eat and live in leaf litter disappeared? 3. 4. Which leaf litter collection in the classroom has the great species richness? Why? 5. Which litter organism in the classroom has the greatest species evenness ratio? 6. Which litter organism had the lowest species evenness ratio? 7. What was the Simpson’s Diversity Index for your sample? Does this indicate a low or high level of diversity? If the diversity was low, why might that be so? 8. Describe the differences in the Simpson’s Diversity Index among the different student groups in your class. Was there a great deal of difference in the calculated index? Does this surprise you? 9. If there were differences in the calculated index among the student groups, give some reasons for this variation.