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CAT DISSECTION LIST Name ____________________ Period _____ Human Anatomy & Physiology 1. Superficial muscles of the thorax and forelimb, ventral view / 14 ______ 2. Superficial muscles of the neck, ventral view / 11 ______ 3. Superficial muscles of the thorax and forelimb, dorsal view / 15 ______ 4. Deep muscles of the thorax, ventral view / 14 ______ 5. Deep muscles of the shoulder and forelimb, dorsal view / 11 ______ 6. Superficial muscles of the hind limb, lateral view / 11 ______ 7. Superficial muscles of the hind limb, medial view / 10 ______ 8. The abdominal and thoracic viscera, ventral view / 20 ______ 9. Visceral surface of the liver / 8 ______ 10. The stomach and colon / 19 ______ 11m. The kidneys and abdominal vessels in a male / 14 ______ 11f. The kidneys and abdominal vessels in a female / 14 ______ 12. Frontal section of the kidney / 8 ______ 13. The brachial vessels and nerves, ventral view / 20 ______ 14. Dorsal view of the heart of the cat / 17 ______ total points = / 192 You get one chance to identify the structures for each of the 14 lists above. Scores will be recorded with a date and initial. For more details, see the ‘Assessment’ section of the Cat Dissection Overview. Good luck Cat Dissection Overview Cat dissection?!? (purpose): ‘Is dissecting cats really necessary?’ you ask. Well, perhaps not when compared with the basic needs of shelter, food, clothing and relationships , but the hands-on experience of systematically examining the various muscles, arteries, viscera and other systems proves to be a very relevant and important application of the topics we have been and will be learning in this course. While there are obvious differences between humans and cats, many of our structures are quite similar (such as the names and purposes of these structures, including muscles, organs, etc.) Dissection is serious work: The work of dissection is serious business and we will take it seriously. We are not Dr. Frankenstein looking to piece together a new creature, nor are we rogue plastic surgeons or amputation specialists looking to slash here and there for a thrill. If you are unable to take your role as a scientist (anatomist/physiologist) seriously then you will no longer be able to participate in this experience. Where/how do ‘they’ get the cats? The cats sold from reputable biological supply companies (such as from which we have purchased our cats) are generally obtained from animal shelters where they were set to be euthanized (put down). Rather than having these animals go to waste, the shelters and the companies have found a triple purpose use by which the cats are disposed of, some money is made, and some valuable learning is accomplished all at the same time. In other words, these cats lived real cat lives and each one will likely be somewhat unique as we explore them. The cats are all adults, some will be males and some will be females, some will still have food in their stomachs (and waste in their large intestine), some may be pregnant, and others may have injuries that you may discover as you go. Safety considerations: There are many ways you could be hurt, etc. but by taking some reasonable (some might say obvious) safety precautions we will all have a safe and enjoyable time. In the end, you (and your lab team) are the only person(s) who can ensure your safety. Exercising the utmost care when using and handling scalpels or other dissection instruments is of the utmost importance – whether your hands are on or off the cat always be aware of what your hands are doing, and also be aware of the hands of others! It should also go without saying that we do not eat in the lab . Since classrooms (especially science classrooms) these days are very overcrowded places, be very careful when moving around the room. On dissection days we will also place all backpacks, coats, and other materials not needed for the dissection under the lab counters around the perimeter of the classroom. Setup/Cleanup procedures: Because we do not have a separate lab space, we will be performing the dissections in our classroom on the available lab counters and table-desks. In other words, this period’s dissection surface is next period’s writing surface . Therefore, at the conclusion of the allotted work time you will be asked to throw away all garbage, scraps, etc. and then return your cat to its bag and then to its storage tote. You should then rinse your dissection equipment at the sink (for scalpels, simply rinse with water and do not touch the blade with your fingers!!!) You should also wash your lab/desk/table surface with soap and water. Remember: the cleaner you are the less you will have to clean! Dissection schedule: We will spend approximately 10 class periods doing dissection. These will take place during most (probably all) class meetings that fall on Mondays, Tuesdays, and Wednesdays between now and Spring Break. With 14 different dissection tasks to complete, you must average more than one completed task each dissection period. Plan to come every day and always come prepared to work! Believe it or not, life (and class) goes on even if you are not here so…if you miss class…upon your return it is your group’s (and your) responsibility to get you caught up. Assessment: There will be a quiz for each of the 14 dissection tasks in your dissection guide. This quiz will be in an oral format. You may be given the name of a structure and asked to identify it on your cat or vice versa (e.g. ‘Please point to the xiphihumeralis on your cat’ or ‘What muscle is this pin in?’). You may alternately be asked about the function of the structures you are studying (e.g. ‘Is the triceps brachii a flexor or an extensor?’ or ‘What two structures are connected by the clavobrachialis?’) Quiz scores will be recorded/initialed/dated on your ‘Cat Dissection List’ sheet so it is advisable that you do not lose this page as it will be the only record of your work until it is turned in! Homework/studying: You can improve your performance during the dissection class periods (and on the quizzes ) by doing some work ahead of time. You will be given your own copy of the dissection guide and a copy will be available for download via the class website. At times you will be given specific homework assignments, such as defining certain words, etc. but in general it is a good idea to read the relevant pages in the dissection guide before you come to class. This way, you will better know what you are supposed to do and may even get a head start on memorizing the names and locations of the structures you are looking at! Equipment: It is advisable that you wear a lab coat, apron, scrubs, etc. to protect your clothing from incidental contact with the cat and/or various fluids that will inevitably be present during the dissection. There will be some lab aprons provided, but if you want to bring your own you are welcome to do that. (Cleaning note: no permanent harm will come to your lab coat, even if they get cat material/liquid on them…a wash in the washing machine with normal laundry detergent will clean them right up!) Ladies and longer haired folk…hair restraints for your long hairs are strongly encouraged – you don’t want those lovely locks falling into the cat and then smelling like embalming fluid for the rest of the day Choosing a group: Your lab group, consisting of four members, will be of your own choosing. However, you are advised to consider all of the factors we have discussed here before agreeing to whom you will work with for the next several weeks! Donation Request: Sunset High School is able to provide the specimens (cats = approx. $40 each) and equipment (scalpels, probes, etc.) for this dissection experience. However, since we will be spending 10 or more class periods doing dissection this means lots of hands needing lots of gloves. Therefore it would be helpful if each student (for whom it is not a financial hardship) could bring a box of nitrile (non-powdered) exam gloves for shared use during the class. These can be found at Fred Meyer, Costco or other local stores. A list will not be kept of who brings or does not bring gloves, we simply ask that you contribute if you are able. (NOTE: Please do not bring in latex gloves as some students may have allergies) CAT DISSECTION GUIDE Human Anatomy & Physiology Table of Contents 1. Superficial muscles of the thorax and forelimb, ventral view 2. Superficial muscles of the neck, ventral view 3. Superficial muscles of the thorax and forelimb, dorsal view 4. Deep muscles of the thorax, ventral view 5. Deep muscles of the shoulder and forelimb, dorsal view 6. Superficial muscles of the hind limb, lateral view 7. Superficial muscles of the hind limb, medial view 8. The abdominal and thoracic viscera, ventral view 9. Visceral surface of the liver 10. The stomach and colon 11m. The kidneys and abdominal vessels in a male 11f. The kidneys and abdominal vessels in a female 12. Frontal section of the kidney 13. The brachial vessels and nerves, ventral view 14. Dorsal view of the heart of the cat 1 SUPERFICIAL MUSCLES OF THE THORAX AND FORELIMB, VENTRAL VIEW 1. brachioradialis 2. clavobrachialis 3. clavotrapezius 5. epitrochlearis 8. external oblique 12. latissimus dorsi 13. palmaris longus 14. pectoantebrachialis 15. pectoralis major 16. pectoralis minor 18. tranverse carpal ligament 19. triceps brachii 20. sternomastoid 21. xiphihumeralis Make your cat look just like the picture. Only skin the cat’s left arm and chest, leaving the right side as protection in case you damage the muscles in preparation. Try to remove as much of fascia and fat as possible. The muscles of the chest are paper-thin as a cat does not use these muscles very much, thus they are not overly developed. The arm will have fascia and protective membranes around the muscles. It is advisable to remove this. Once removed, if you press on the arm the muscles will separate out and become easier to see. 2 SUPERFICIAL MUSCLES OF THE NECK, VENTRAL VIEW 1. anterior facial vein 3. clavicle 4. clavobrachialis 5. clavotrapezius 8. external jugular vein 14. pectoantebrachialis 15. pectoralis major 16. posterior facial vein 17. sternohyoid 18. sternomastoid 20. transverse jugular vein 3 SUPERFICIAL MUSCLES OF THE THORAX AND FORELIMB, DORSAL VIEW 1. acromiodeltoid 2. acromiotrapezius 3. brachioradialis 4. clavobrachialis 5. clavotrapezius 6. dorsal carpal ligament 9. extensor carpi ulnaris 12. infraspinatus 13. lateral head of triceps 14. latissimus dorsi 15. levator scapulae ventralis 16. long head of triceps 17. spinodeltoid 18. spinotrapezius 19. teres major 4 DEEP MUSCLES OF THE THORAX, VENTRAL VIEW 1. biceps brachii 2. brachialis 4. cut insertions of pectoralis major & pectoantebrachialis 5. epitrochliaris 6. external oblique 7. latissimus dorsi 9. pectoralis minor 10. rectus abdominis 12. serratus ventralis 13. sternohyoid 14. subscapularis 15. teres major 17. triceps brachii 18. xiphihumeralis 5 DEEP MUSCLES OF THE SHOULDER AND FORELIMB, DORSAL VIEW 2. brachialis 12. head of the humerus 13. infraspinatus 14. long head of triceps 15. medial head of triceps 16. rhomboideus 18. serratus dorsalis 19. serratus ventralis 20. spine of scapula 23. supraspinatus 24. teres major brachialis 6 SUPERFICIAL MUSCLES OF THE HINDLIMB, LATERAL VIEW 1. aponeurosis of tensor faciae latae 2. biceps femoris 3. caudofemoralis 6. fascia lata 7. gastrocnemius 8. gluteus maximus 9. lateral malleolus 15. sartorius 17. tensor faciae latae 18. tibialis anterior 20. semitendinosus SUPERFICIAL MUSCLES OF THE HINDLIMB, MEDIAL VIEW 2. adductor femoris 4. external oblique 7. gastrocnemius 8. gracilis 10. medial malleolus 12. rectus abdominus 13. sartorius 14. semitendinosis 16. tibia 17. tibialis anterior 7 8 VISCERAL SURFACE OF THE LIVER 9 1. caudate lobe 2. coronary ligament 3. left medial lobe 4. nonperitoneal surface 5. postcava 6. right lateral lobe, posterior part 8. right medial lobe To remove the liver you must sever the postcava on the superior and separate the diaphragm. There are lobes that are hidden underneath so carefully locate those and include those with your dissection. When you remove the liver you will also remove the gall bladder and common bile duct. The liver produces bile, which cleanses food and neutralizes the stomach acids – the common bile duct dumps the bile (stored in the gall bladder) into the duodenum. 10 THE STOMACH AND COLON 2. aorta 3. ascending colon 4. caudate lobe of liver 5. cecum 7. common bile duct 9. descending colon 10. descending limb of duodenum 18. jejunum 20. left lateral lobe of liver 22. mesentery 23. mesocolon 25. pancreas 27. portal vein 33. splenic artery 34. spleen 35. stomach 36. superior mesenteric artery 37. superior mesenteric vein 38. transverse colon THE KIDNEYS AND ABDOMINAL VESSELS IN A MALE 11 3. aorta 4. branch of deep femoral artery and vein 6. colon 7. diaphragm 9. esophagus 12. kidney 14. iliopsoas 18. postcava 19. psoas minor 20. renal artery 21. renal vein 22. spermatic chord 25. ureter 26. urinary bladder Notice the picture shows the colon removed. I recommend not severing the colon because it is full of feces. Simply push the colon to the side and proceed m 11 f THE KIDNEYS, UTERUS, AND ABDOMINAL VESSELS IN A FEMALE 4. aorta 5. branches of deep femoral artery and vein 8. colon 9. diaphragm 10. esophagus 11. fallopian tube 13. hepatic veins 18. kidney 19. uterine horn 23. ovarian artery and vein 24. ovary 25. post cava 27. renal artery 28. renal vein 36. ureter 37. urinary bladder Notice the picture shows the colon removed. I recommend not severing the colon because it is full of feces. Simply push the colon to the side and proceed. 12 13 THE BRACHIAL VESSELS AND NERVES, VENTRAL VIEW 2. aortic arch 5. axillary vein 6. biceps 8. brachial vein 10. common carotid artery 13. esophagus 14. external jugular vein 16. innominate artery 17. innominate vein 19. internal jugular vein 26. left ventricle 35. postcava 36. precava 37. pulmonary artery 39. right atrium 40. right lung, anterior lobe 41. right lung, middle lobe 42. right ventricle 53. trachea 55. triceps Make the cat’s chest cavity look just like the picture (see next page). Find the precava and innominate artery and clean all the chest muscle away. Follow those two vessels up to the neck, clearing away all the muscles and tissue as you go. Everywhere that the vessels branch, clean those and follow them. Expose the vessels into the arm. Make the trachea and carotid arteries and jugular veins accessible. Remove the left lungs to expose the pulmonary vessels. 13 14 4 9 13 11 15 7 16 8 5 16 8 14 6 3 3 DORSAL VIEW OF THE HEART OF THE CAT 1. aorta 2. azygos vein 3. dorsal pulmonary vein 4. innominate artery 5. left atrium 6. left auricle 7. left pulmonary artery 8. left pulmonary veins 9. left subclavian artery 10. left ventricle 11. ligamentum areteriosum 12. postcava 13. precava 14. right atrium 15. right pulmonary artery 16. right pulmonary vein 17. right ventricle 12 10 To remove the heart from the cat you have to sever the pulmonary arteries and veins that connect to the lungs (remove the lungs). Also sever the aorta about 1 full inch from the heart – this insures that the innominate and left subclavian arteries stay intact. Also try to include about ½ inch of the innominate and left subclavian artery with the heart. Sever the precava and the postcava and the heart should lift out. The heart is surrounded by a membranous sack called the pericardium – this should be carefully removed. Also part of the esophagus will be connected to the heart and should be removed. Remember that arteries are usually red because they carry oxygenated blood away from the heart and veins are blue because they are deoxygenated blood back to the heart. The pulmonary veins and arteries are the exception. Pulmonary arteries are blue because they are carrying the blood to the lungs to receive oxygen and pulmonary veins carry the oxygen rich blood back to the heart. (In your cat the arteries and veins are red and blue because they have colored latex injected into them )