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SGUL/LSHTM MRC London Intercollegiate Doctoral Training Partnership – 2017/18 Additional Studentships – Potential PhD Projects Title of PhD project 3D mapping of cancer metabolism using nano-sensors: a new approach to cancer diagnosis Supervisor Dr Elena Sviderskaya SGUL Co-Supervisor Professor Yuri Korchev Imperial College London Brief description of project The metabolic microenvironment surrounding tumours dramatically influences their growth, proliferation, metastatic potential and response/resistance to treatment. Melanocyte transformation into cancer is associated with significant structural alterations in melanosomes, which protect the cell by scavenging free radicals generated, by sunlight and cellular metabolism. This makes melanoma especially important type of cancer to evaluate changes in extracellular acidity (pH) and production of ATP (adenosine triphosphate) and ROS (reactive oxygen species). We aim to optimise and apply a novel class of field-effect transistor (FET) nanosensors to elucidate the role of metabolic and oxidative stress in melanoma. The FET nano-sensors allow long term and sensitive monitoring of these key cancer biomarkers in cells, 3D mapping in tissue and can help in early cancer diagnosis. This is a joint project with Imperial College Medical School. Particular prior educational requirements for a student undertaking this project None Skills we expect a student to develop/acquire whilst pursuing this project MRC skills priority - Interdisciplinary skills (physical/engineering including imaging and cell biology). Cell biology skills: Advanced mammalian cell culture (cells of melanocytic lineage), cell biology and molecular biology methods including cell proliferation, differentiation, senescence and apoptosis assays, immunostaining; Physical/engineering skills: Advanced high-resolution microscopy for live cell imaging and design of nano-sensors for analysis of tumour samples. 1