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MarBEF TRAINING COURSE ON BIOASSAY METHODS IN MARINE CHEMICAL ECOLOGY September 9-14, 2006 Tjärno Marine Biological Laboratory, Strömstad, Sweden (www.tmbl.gu.se) PROGRAM Fri 8th 17:30 Arrival and registration Dinner Sat 9th MICROBES 9:00 – 10:00 Jens HARDER 10:00 – 10:30 10:30 – 11:00 Coffee Jens HARDER 11:00 – 12:30 Friedericke HOFFMANN 12:30 – 14:00 14:00 – 15:00 15:00 – 15:30 15:30 – 16:30 16:30 – 17:30 17:30 – 18:30 18:30 - Lunch Preparation of agar plates HARDER/HOFFMANN Coffee DAPI staining and epifluorescence microscopy HARDER/HOFFMANN DAPI staining and epifluorescence microscopy HARDER/HOFFMANN Dinner Welcome mixer with guided tour of TMBL General microbiology, secondary metabolites and their function General microbiology, secondary metabolites and their function (cont’d) Culture-independent techniques, interspecies chemical communication: examples from prokaryotes and eukaryotes Sun 10th BENTHIC INVERTEBRATES 9:00 – 10:00 Joe PAWLIK 10:00 – 10:30 10:30 – 11:30 11:30 – 12:30 12:30 – 14:00 14:00 – 15:00 Coffee Angelo FONTANA Ernesto MOLLO Lunch PAWLIK/ FONTANA 15:00 – 15:30 15:30 – 16:30 Coffee MOLLO/FONTANA Antifeedant activity, Ichithyotoxicity, Antifouling activity 16:30 – 17:30 MOLLO/FONTANA Antifeedant activity, Ichithyotoxicity, Antifouling activity 17:30 – Dinner Historical perspective on studies of invertebrate chemical defenses, development of assay methods, ecological relevance and limitations. Chemical mediators in marine opisthobranchs A chemoecological approach to biological invasions Volume-based gel techniques for lab and field assays testing questions of chemical defense, fouling and overgrowth. Mo 11th PHYTOPLANKTON 9:00 – 10:00 Catherine LEGRANDE and Raffaella CASOTTI 10:00 – 10:30 10:30 – 11:30 Coffee LEGRAND/CASOTTI 11:30 – 12:30 Michael STEINKE 12:30 – 14:00 14:00 – 15:00 15:00 – 15:30 15:30 – 16:30 16:30 – 17:30 17:30 – Lunch LEGRAND Coffee LEGRAND/STEINKE LEGRAND/STEINKE Dinner Tue 12ve SEAWEEDS 9:00 – 11:00 Mark HAY 11:00 – 14:00 14:00 – 15:00 Boat excursion and lunch Philippe POTIN 15:00 – 15:30 15:30 – 16:30 Coffee Gunilla TOTH 16:30 – 18:30 POTIN 19:30 – Social dinner Wed 13th ZOOPLANKTON 9:00 – 10:00 Adrianna IANORA 10:00 – 10:30 11:00 – 11:30 Coffee Serge POULET 11:30 – 12:30 Maarten BOERSMA 12:30 – 14:00 14:00 – 15:00 15:00 – 15:30 15:30 – 16:30 16:30 – 17:30 Lunch IANORA/ROMANO Coffee Giovanna ROMANO POULET/BOERSMA 17:30 Dinner Thu 14th Departure Allelochemicals and toxins in phytoplankton and bioassay methods in phytoplankton: from fluorescent molecules to large-scale ecosystem manipulations Allelochemicals and toxins in phytoplankton and bioassay methods in phytoplankton: from fluorescent molecules to large-scale ecosystem manipulations Microzooplankton grazing and the production of biogenic trace gases Vital staining of phytoplankton Microzooplankton grazing Microzooplankton grazing Ecologically realistic bioassays as the key for progress in marine chemical ecology: some problems, some solutions.... some more problems Bioassay methods to study induced defenses and resistance in seaweeds. Different bioassays used to study plant-herbivore interactions Bioassays of induced resistance in seaweeds and tests of ROS and metabolite toxicity in seaweeds Bioassay methods to study the effects of toxic dinoflagellate metabolites on feeding and reproduction of zooplankton Bioassay methods to study the effects of diatom metabolites on the reproductive biology of copepods Defence mechanisms in the pelagic zones: which explanatory models are most adequate. Toxicological tests on marine invertebrates Toxicological tests on marine invertebrates Classic and modern methods to observe and compare the effects of phytoplankton diets on zooplankton