Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Do-Coop Technologies Ltd Name of document: Rapid OneStep tissue/swab Extraction protocol Version #: Document #:1 Page 1 of 2 Pages Copy #:1 Written by: Chen Guttman Effective date: Approved by: Rapid OneStep Tissue/Swab Extraction protocol Introduction Rapid OneStep Tissue/Swab Extraction protocol is intended for use with fresh tissue or swab samples and yields appropriate amounts of DNA for successful amplification of targets via PCR and qPCR methods. Protocol 1. Add 100µl of Rapid OneStep Tissue/Swab Extraction buffer I for every 3x3 mm fresh tissue sample or one swab pre-seprated from stick by hand or scissors. Make sure the pieces of swab are submerged underneath the extraction buffer 2. Incubate samples at 95°c for 10 minutes. 3. Centrifuge sample at > 10,000 rpm for one minute and transfer supernatant to a fresh tube. 4. Add approximately 30% of Rapid OneStep Tissue/Swab Extraction buffer II to the sample and briefly vortex mixture. 5. Samples can be kept for several days at 4°c or for several months at -20°c. Storage Rapid OneStep Tissue/Swab Extraction buffer should be kept at Room Temperature. Rapid OneStep Tissue/Swab Extraction protocol Introduction Do-Coop Technologies Ltd. 3B Yoni Netanyahu St., Or-Yehuda ISRAEL 60376 Tel: +972 3-5333804 Fax: +972 3-5333847 www.docoop.com Do-Coop Technologies Ltd Name of document: Rapid OneStep tissue/swab Extraction protocol Version #: Document #:1 Page 2 of 2 Pages Copy #:1 Written by: Chen Guttman Effective date: Approved by: Rapid OneStep Tissue/Swab Extraction protocol is intended for use with fresh tissue or swab samples and yields appropriate amounts of DNA for successful amplification of targets via PCR and qPCR methods. Protocol 1. Cur the lower portion (root) of one to three hair/freshly plucked samples (5 mm) 2. Add 100µl of Rapid OneStep Tissue/Swab Extraction buffer I and make sure the pieces are submerged underneath the extraction buffer 3. Incubate samples at 95°c for 10 minutes. 4. Centrifuge sample at > 10,000 rpm for one minute and transfer supernatant to a fresh tube. 5. Add approximately 30% of Rapid OneStep Tissue/Swab Extraction buffer II to the sample and briefly vortex mixture. 6. Samples can be kept for several days at 4°c or for several months at -20°c. Storage Rapid OneStep Tissue/Swab Extraction buffer should be kept at Room Temperature. Do-Coop Technologies Ltd. 3B Yoni Netanyahu St., Or-Yehuda ISRAEL 60376 Tel: +972 3-5333804 Fax: +972 3-5333847 www.docoop.com